We report the direct excitation of the highly forbidden (6s 2 ) 1 S0 ↔ (6s6p) 3 P0 optical transition in two odd isotopes of ytterbium. As the excitation laser frequency is scanned, absorption is detected by monitoring the depletion from an atomic cloud at ∼70 µK in a magneto-optical trap. The measured frequency in 171 Yb (F=1/2) is 518,295,836,593.2 ± 4.4 kHz. The measured frequency in 173 Yb (F=5/2) is 518,294,576,850.0 ± 4.4 kHz. Measurements are made with a femtosecond-laser frequency comb calibrated by the NIST cesium fountain clock and represent nearly a million-fold reduction in uncertainty. The natural linewidth of these J=0 to J=0 transitions is calculated to be ∼10 mHz, making them well-suited to support a new generation of optical atomic clocks based on confinement in an optical lattice.PACS numbers: 32.30. Jc, 06.30.Ft, 32.80.Pj, 39.30.+w Work is underway to realize a high-performance optical clock that combines the best features of state-of-the-art single-ion and neutral atom optical frequency standards [1]. Such a clock system comprises a dipole-force optical lattice trap that confines an ensemble of neutral atoms individually to sub-wavelength sites for Dopplerand recoil-free precision spectroscopy [2,3]. Crucial to this scheme is an atom with a "clock" transition that has both a narrow linewidth and an insensitivity to lattice perturbations. Several groups have recognized ytterbium as an excellent candidate and are pursuing lattice-based optical clocks that will use its narrow 1 S 0 ↔ 3 P 0 transition [3,4,5,6]. However, until now the absolute frequency of this resonance was known in tables [7] to only a few gigahertz. We report the direct excitation of the doubly-forbidden (6s 2 ) 1 S 0 ↔ (6s6p) 3 P 0 optical transition at 578.4 nm in two odd isotopes of ytterbium [8]. Using a femtosecond-laser frequency comb [9], we make precision absolute frequency measurements with an uncertainty of 4.4 kHz -an improvement of nearly 106 . This accurate frequency knowledge will expedite the pursuit of an ytterbium-based optical clock, whose performance can potentially surpass that of the best cesium primary standard [10] by orders of magnitude.The ytterbium 1 S 0 ↔ 3 P 0 resonance is an outstanding potential clock transition, in part because of its narrow natural linewidth (∼10 mHz [3]). This transition is strictly dipole-forbidden from spin and orbital angular momentum considerations. An appreciable excitation probability exists, however, through hyperfine mixing of the 3 P 0 level with nearby states in the odd isotopes. (See the diagram in Fig. 1.) In an optical atomic clock based on confinement to a lattice, the lattice laser wavelength is chosen such that the ac Stark shift of the upper clock energy state matches the shift of the ground state. This results in a vanishing net perturbation to the clock frequency. The shiftcanceling wavelength for ytterbium is calculated to be 752 nm [3], readily accessible by high-power cw titaniumsapphire laser systems. Furthermore, the J=0 to J=0 transitions in Yb repor...