Cloacibacillus sp., a Potential Human Pathogen Associated with Bacteremia in Quebec and New Brunswick

Domingo, Marc-Christian; Yansouni, Cédric P.; Gaudreau, Christiane; Lamothe, F; Lévesque, Simon; Tremblay, Claude; Garceau, Richard

doi:10.1128/jcm.01137-15

Cited by 21 publications

(13 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study we sequenced, annotated and analysed genomes of 133 different anaerobes cultured from the chicken caecum. Several isolates represented species which are available in only a few pure cultures worldwide – a single manuscript reported a pure culture of Elusimicrobium [ 19 ] and only two papers reported culture of Cloacibacillus porcorum [ 20 , 21 ]. Although such isolates are of clear potential for future experiments, in this report we mainly focused on the comparison of genetic potential of isolates belonging to two main phyla inhabiting the intestinal tract of chickens, Bacteroidetes and Firmicutes [ 8 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing and function prediction of 133 gut anaerobes isolated from chicken caecum in pure cultures

et al. 2018

View full text Add to dashboard Cite

BackgroundIn order to start to understand the function of individual members of gut microbiota, we cultured, sequenced and analysed bacterial anaerobes from chicken caecum.ResultsAltogether 204 isolates from chicken caecum were obtained in pure cultures using Wilkins-Chalgren anaerobe agar and anaerobic growth conditions. Genomes of all the isolates were determined using the NextSeq platform and subjected to bioinformatic analysis. Among 204 sequenced isolates we identified 133 different strains belonging to seven different phyla - Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Verrucomicrobia, Elusimicrobia and Synergistetes. Genome sizes ranged from 1.51 Mb in Elusimicrobium minutum to 6.70 Mb in Bacteroides ovatus. Clustering based on the presence of protein coding genes showed that isolates from phyla Proteobacteria, Verrucomicrobia, Elusimicrobia and Synergistetes did not cluster with the remaining isolates. Firmicutes split into families Lactobacillaceae, Enterococcaceae, Veillonellaceae and order Clostridiales from which the Clostridium perfringens isolates formed a distinct sub-cluster. All Bacteroidetes isolates formed a separate cluster showing similar genetic composition in all isolates but distinct from the rest of the gut anaerobes. The majority of Actinobacteria clustered closely together except for the representatives of genus Gordonibacter showing that the genome of this genus differs from the rest of Actinobacteria sequenced in this study. Representatives of Bacteroidetes commonly encoded proteins (collagenase, hemagglutinin, hemolysin, hyaluronidase, heparinases, chondroitinase, mucin-desulfating sulfatase or glutamate decarboxylase) that may enable them to interact with their host. Aerotolerance was recorded in Akkermansia and Cloacibacillus and was also common among representatives of Bacteroidetes. On the other hand, Elusimicrobium and the majority of Clostridiales were highly sensitive to air exposure despite their potential for spore formation.ConclusionsMajor gut microbiota members utilise different strategies for gut colonisation. High oxygen sensitivity of Firmicutes may explain their commonly reported decrease after oxidative burst during gut inflammation.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4959-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing and function prediction of 133 gut anaerobes isolated from chicken caecum in pure cultures

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Patient 8, a patient with developmental delay and limitation of support, had a suspected infection that clinicians were unable to identify, ultimately resulting in the patient passing away from MODS several days following the study. Using the bacterial mapping, we isolated unique signatures of Cloacibacillus in all three days with a further elevation at day 8, a bacteria incredibly challenging to grow and shown to associate with multi-organ pathology similar to this patient (13) . While reads align to some of these transcriptomes, reads may belong to closely related species not within our transcriptome database.…”

Section: Resultsmentioning

confidence: 99%

“…(MODS-Sepsis; MV; PELOD-20): Died; Seizure disorder with identified variants in CRELD1 (p.Q320RfsX25, p.C192Y); Clinically confirmed Metapneumo; Raoultella and Cloacibacillus enriched in all three days of RNAseq with Cloacibacillus highest at day8 ( Cloacibacillus has been associated with multi-organ pathology in multiple cases (13) ); elevation of 72 transcripts at all days relative to other patients suggestive of neutrophil degranulation (1e-12), leukocyte activation (FDR 6e-13), and linked with known neutrophil protease associated organ pathologies (21–24) . Second highest PERSEVERE gene elevation.…”

Section: Resultsmentioning

confidence: 99%

Viral Induced Genetics Revealed by Multi-Dimensional Precision Medicine Transcriptional Workflow

Prokop

Gupta

Leimanis

et al. 2020

Preprint

View full text Add to dashboard Cite

Precision medicine requires the translation of basic biological understanding to medical insights, mainly applied to characterization of each unique patient. In many clinical settings, this requires tools that can be broadly used to identify pathology and risks. Patients often present to the intensive care unit with broad phenotypes, including multiple organ dysfunction syndrome (MODS) resulting from infection, trauma, or other disease processes. Etiology and outcomes are unique to individuals, making it difficult to cohort patients with MODS, but presenting a prime target for testing/developing tools for precision medicine. Using multi-time point whole blood (cellular/acellular) total transcriptomics in 27 patients, we highlight the promise of simultaneously mapping viral/bacterial load, cell composition, tissue damage biomarkers, balance between syndromic biology vs. environmental response, and unique biological insights in each patient using a single platform measurement. Integration of a transcriptome workflow yielded unexpected insights into the complex interplay between host genetics and viral/bacterial specific mechanisms, highlighted by a unique case of virally induced genetics (VIG) within one of these 27 patients. The power of RNAseq to study unique patient biology while investigating environmental contributions can be a critical tool moving forward for translational sciences applied to precision medicine.

show abstract

“…Fecal samples produced similar results where strains from multiple genera, including Bacteroides, Cloacibacillus, and Ruminococcus were found commonly in captive samples but absent in rarefied, noncaptive reads (Appendix S2). These three genera are also potentially pathogenic strains in humans (Domingo et al, 2015;Titécat et al, 2014;Wexler, 2007). Although determining the exact pathogenic capacities of particular microbes is outside the realm of this investigation, high abundances of potential pathogens in animals under human care support the possibility that headstart animals can harbor diseasecausing bacteria at significantly higher rates than animals living in the wild (Redford et al, 2012).…”

Section: Discussionmentioning

confidence: 97%

Gut microbial ecology of the Critically Endangered Fijian crested iguana (Brachylophus vitiensis): Effects of captivity status and host reintroduction on endogenous microbiomes

Eliades

Brown

Colston

et al. 2021

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

Cloacibacillus sp., a Potential Human Pathogen Associated with Bacteremia in Quebec and New Brunswick

Cited by 21 publications

References 14 publications

Whole genome sequencing and function prediction of 133 gut anaerobes isolated from chicken caecum in pure cultures

Whole genome sequencing and function prediction of 133 gut anaerobes isolated from chicken caecum in pure cultures

Viral Induced Genetics Revealed by Multi-Dimensional Precision Medicine Transcriptional Workflow

Gut microbial ecology of the Critically Endangered Fijian crested iguana (Brachylophus vitiensis): Effects of captivity status and host reintroduction on endogenous microbiomes

Contact Info

Product

Resources

About