Cloacal<i>Lactobacillus</i>Isolates from Broilers Often Display Resistance Toward Tetracycline Antibiotics

Cauwerts, Katty; Pasmans, Frank; Devriese, Luc; Haesebrouck, Freddy; Decostere, Annemie

doi:10.1089/mdr.2006.12.284

Cited by 22 publications

(29 citation statements)

References 37 publications

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“…strains (35,39) and also for cloacal Lactobacillus salivarius subsp. salivarius isolates (5). However, to the best of our knowledge this is the first report on the coexistence of two genes encoding different mechanisms of Tc r in the same L. sakei strain.…”

Section: Discussionmentioning

confidence: 74%

“…However, in recent years a number of studies have correlated atypically high phenotypic resistances with the presence of tet genes (11,17,18,19,20,26). Tetracycline resistance in Lactobacillus has commonly been associated with the presence of tet(M) (19,20), but recently the gene coding for the efflux transporter Tet L was also described for some cloacal isolates (5). However, data about the functionality of both genes when they coexist in the same bacterium were not available until now.…”

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confidence: 99%

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Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Ammor

Gueimonde

Danielsen

et al. 2008

Appl Environ Microbiol

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Lactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. sakei Rits 9, a strain isolated from Italian Sola cheese made from raw milk, is mediated by a transposon-associated tet(M) gene coding for a ribosomal protection protein and a plasmidcarried tet(L) gene coding for a tetracycline efflux pump. pLS55, the 5-kb plasmid carrying the tet(L) gene, is highly similar to the pMA67 plasmid recently described for Paenibacillus larvae, a species pathogenic to honeybees. pLS55 could be transferred by electroporation into the laboratory strain L. sakei 23K. While the L. sakei 23K transformant containing pLS55 displayed an intermediate tetracycline resistance level (MIC, <32 g/ml), L. sakei Rits 9, containing both tetracycline-resistant determinants, had a MIC of <256 g/ml, suggesting that Tet L and Tet M confer different levels of resistance in L. sakei. Remarkably, in the absence of tetracycline, a basal expression of both genes was detected for L. sakei Rits 9. In addition, subinhibitory concentrations of tetracycline affected the expression patterns of tet(M) and tet(L) in different ways: the expression of tet(M) was induced only at high tetracycline concentrations, whereas the expression of tet(L) was up-regulated at lower concentrations. This is the first time that two different mechanisms conferring resistance to tetracycline are characterized for the same strain of a lactic acid bacterium.

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Section: Discussionmentioning

confidence: 74%

mentioning

confidence: 99%

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Ammor

Gueimonde

Danielsen

et al. 2008

Appl Environ Microbiol

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“…Numerous studies have already shown that TET resistance is typically transferable and a variety of plasmids have been observed (Boguslawska et al 2009;Kazimierczak et al 2009). There are currently 38 different tet resistance genes described (Cauwerts et al 2006). Gene tet(M) encoding ribosomal protection and gene tet(L) determining efflux transporter have been the most often genes identified in poultry enterococci (Boguslawska et al 2009).…”

Section: Tet Tetracycline Ery Erythromycin Mic Minimal Inhibitory Conmentioning

confidence: 99%

Detection of tetracycline and macrolide resistance determinants in enterococci of animal and environmental origin using multiplex PCR

2011

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An occurrence of resistance to tetracycline (TET) and erythromycin (ERY) was ascertained in 82 isolates of Enterococcus spp. of animal and environmental origin. Using E test, 33 isolates were resistant to TET and three isolates to ERY. Using polymerase chain reaction (PCR; single and multiplex), the TET determinants tet(M) and tet(L) were detected in 35 and 13 isolates, respectively. Twelve isolates carried both tet(M) and tet(L) genes. Eight isolates possessed ermB gene associated with ERY resistance. Multiplex PCR was shown to be a suitable method for simultaneous determination of all three resistance determinants that occurred most frequently in bacteria isolated from poultry. This study also demonstrates that gastrointestinal tract of broilers may be a reservoir of enterococci with acquired resistance to both TET and ERY that can be transferred to humans via food chain.

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“…For example, L. agilis (7,57,62), L. amylovorus (11), L. crispatus (27,62), L. gallinarum (27,28,62), L. johnsonii (27,57,62), L. panis (43), L. reuteri (27,43,62), L. salivarius (27,62), L. saerimneri (62), L. vaginalis (7,57,62), P. acidilactici (1,38), P. pentosaceus (53), Enterococcus cecorum (7), E. faecium (26,31), E. faecalis (46), Enterococcus villorum (61), and Veillonella spp. (69) have previously been described as inhabitants of the chicken GIT.…”

Section: Comparison Of the Rep-pcr Protocols For Representativementioning

confidence: 99%

Comparison and Utilization of Repetitive-Element PCR Techniques for Typing Lactobacillus Isolates from the Chicken Gastrointestinal Tract

Stephenson

Moore

Allison

2009

Appl Environ Microbiol

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Three repetitive-element PCR techniques were evaluated for the ability to type strains of Lactobacillus species commonly identified in the chicken gastrointestinal tract. Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) produced species-and strain-specific profiles for Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus johnsonii, and Lactobacillus reuteri isolates. The technique typed strains within these species equally as well as pulsed-field gel electrophoresis. DNA concentration and quality did not affect the ERIC-PCR profiles, indicating that this method, unlike other high-resolution methods, can be adapted to high-throughput analysis of isolates. Subsequently, ERIC-PCR was used to type Lactobacillus species diversity of a large collection of isolates derived from chickens grown under commercial and necrotic enteritis disease induction conditions. This study has illustrated, for the first time, that there is great strain diversity within each Lactobacillus species present and has revealed that chickens raised under commercial conditions harbor greater species and strain diversity than chickens raised under necrotic enteritis disease induction conditions.

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CloacalLactobacillusIsolates from Broilers Often Display Resistance Toward Tetracycline Antibiotics

Cited by 22 publications

References 37 publications

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Detection of tetracycline and macrolide resistance determinants in enterococci of animal and environmental origin using multiplex PCR

Comparison and Utilization of Repetitive-Element PCR Techniques for Typing Lactobacillus Isolates from the Chicken Gastrointestinal Tract

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