Clinicopathological findings of diabetes mellitus induced by alloxan in goats

Haghdoost, I Sohrabi; Ghaleshahi, A. Jamshidian; Safi, Shahabeddin

doi:10.1007/s00580-006-0627-1

Cited by 5 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They equally noted that alloxan has a narrow diabetogenic dose and that overdose could lead to death. Researchers have reported death of experimental goat following alloxan administration (Haghdoost et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Age Susceptibility of Wistar Rats to Alloxan-Induced Diabetes: A Paradox

Patrick

Edeh

2019

Not Sci Biol

View full text Add to dashboard Cite

Selection of appropriate ages of rats for experimental diabetes mellitus studies has been posing great challenges to researchers as the rats may either not come down with diabetes or may even die upon induction. This study investigated the influence of age as a possible factor affecting successful induction of diabetes mellitus in albino Wistar rats. One hundred male albino Wistar rats of varying ages weighing between 23.63 ± 0.46 g and 279.00 ± 27.00 g and assigned into ten (10) groups of ten rats per group were used for the study. Diabetes was induced in groups 1-9 consisting of rats aged 3, 5, 7, 9, 11, 13, 15, 17 and 19 weeks respectively by injecting 160 mg/kg of alloxan monohydrate intraperitoneally while group 10 rats (aged 12 weeks) served as normal control. Fasting blood glucose levels and the weights of the rats were determined on 0 h, 24 h, 48 h, 72 h and 96 h post alloxan monohydrate injection. Body conditions and behaviors of the rats were subjectively scored. Results showed that rats in groups 3-9 came down with hyperglycemia 48 h post-alloxan injection. The body conditions and behaviors of groups 3 (aged 7 weeks) and 4 (Aged 9 weeks) rats compared very well (p<0.05) with those of the normal control rats. It was concluded that rats aged 7-9 weeks were most suitable for use in studies involving induction of experimental diabetes mellitus using alloxan monohydrate at the dose of 160 mg/kg intraperitoneally.

show abstract

Section: Discussionmentioning

confidence: 99%

Age Susceptibility of Wistar Rats to Alloxan-Induced Diabetes: A Paradox

Patrick

Edeh

2019

Not Sci Biol

View full text Add to dashboard Cite

show abstract

“…Although the normoglycemic blood glucose values of goats (50-75 mg/dL range; mean ¼ 62.8 mg/dL) are lower than the human values (80-100 mg/dL range; mean ¼ 72 mg/dL), goats are widely accepted as an animal model for studying human diabetes. 20 The glucose metabolism of goats reacts in a similar fashion as the human glucose metabolism on stimuli like stress, glucose infuse, etc. Moreover, goats can be 'made' diabetic with the use of alloxan or streptozotocin.…”

Section: Discussionmentioning

confidence: 99%

First step toward near-infrared continuous glucose monitoring: in vivo evaluation of antibody coupled biomaterials

Gellynck

Kodeck

Walle

et al. 2014

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Continuous glucose monitoring (CGM) is crucial in diabetic care. Long-term CGM systems however require an accurate sensor as well as a suitable measuring environment. Since large intravenous sensors are not feasible, measuring inside the interstitial fluid is considered the best alternative. This option, unfortunately, has the drawback of a lag time with blood glucose values. A good strategy to circumvent this is to enhance tissue integration and enrich the peri-implant vasculature. Implants of different optically transparent biomaterials (poly(methyl-methacrylate) [PMMA] and poly(dimethylsiloxane) [PDMS]) -enabling glucose monitoring in the near-infrared (NIR) spectrum -were surface-treated and subsequently implanted in goats at various implantation sites for up to 3 months. The overall in vivo biocompatibility, tissue integration, and vascularization at close proximity of the surfaces of these materials were assessed. Histological screening showed similar tissue reactions independent of the implantation site. No significant inflammation reaction was observed. Tissue integration and vascularization correlated, to some extent, with the biomaterial composition. A modification strategy, in which a vascular endothelial-cadherin antibody was coupled to the biomaterials surface through a dopamine layer, showed significantly enhanced vascularization 3 months after subcutaneous implantation. Our results suggest that the developed strategy enables the creation of tissue interactive NIR transparent packaging materials, opening the possibility of continuous glucose monitoring.

show abstract

“…AL enters into the human body on consuming these bakery products made out of bleached flour and becomes a target of AL-induced diabetes. The expected amount of AL limit in the human blood is 1.5 μg/mL but with the increase in AL content, it leads to AL-diabetes, which in turn causes weight loss, polyuria, ketonuria, and polydipsia. , Hence, it is imperative to develop a sensitive, efficient, simple, and cost-effective technique for the determination of AL. Research enthusiasts have given ample consideration for developing methods to quantitatively determine bioactive substances such as AL in food products.…”

Section: Introductionmentioning

confidence: 99%

Design of a Sensitive and Selective Voltammetric Sensor Based on a Cationic Surfactant-Modified Carbon Paste Electrode for the Determination of Alloxan

2020

View full text Add to dashboard Cite

Alloxan (AL) is a toxic glucose analogue that acts as a potent diabetogenic inducer by selectively destroying the insulin-producing β-cells of the pancreas. Hence, a sensitive and selective cetyl trimethylammonium bromide (CTAB)-immobilized carbon paste electrode was utilized for the analysis of AL in the existence of anthrone. The CTAB-modified carbon paste electrode in contrast with the bare carbon paste electrode showed a magnificent behavior for the electrocatalytic oxidation of AL by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. CV studies reveal a quasi-reversible diffusion-controlled process in the potential window of −0.5 to 0.4 V at an optimum pH of 6.5 in 0.2 M phosphate buffer solution. The electrode materials were characterized by CV, field emission-scanning electron microscopy, and electrochemical impedance spectroscopy. Under optimized experimental conditions, low detection limits of 1.09 and 3.64 μM were obtained in a linear dynamic range of 5–80 μM and from 8 to 90 μM by DPV and CV methods, respectively. The performance of the modified electrode is impressive in terms of least charge transfer resistance ( R ct ), surface concentration (Γ), and heterogeneous electron transfer rate constant ( k 0 ). A 50-fold excess concentration of other potential interferants such as food additives and other organic species present in the human body does not significantly alter the peak potential and peak current of AL. The analytical application of the modified sensor was appraised by determining AL in the spiked refined flour sample. The modified sensor with a swift fabrication procedure exhibited enduring stability, adequate reproducibility, and acceptable repeatability.

show abstract

Clinicopathological findings of diabetes mellitus induced by alloxan in goats

Cited by 5 publications

References 5 publications

Age Susceptibility of Wistar Rats to Alloxan-Induced Diabetes: A Paradox

Age Susceptibility of Wistar Rats to Alloxan-Induced Diabetes: A Paradox

First step toward near-infrared continuous glucose monitoring: in vivo evaluation of antibody coupled biomaterials

Design of a Sensitive and Selective Voltammetric Sensor Based on a Cationic Surfactant-Modified Carbon Paste Electrode for the Determination of Alloxan

Contact Info

Product

Resources

About