Clinicopathological characterization of experimental infection in chickens with sub-genotype VIIi Newcastle disease virus isolated from peafowl

Desingu, Perumal Arumugam; Singh, S. D.; Dhama, Kuldeep; Kumar, O.R. Vinodh; Malik, Yashpal Singh; Singh, Raj Kumar

doi:10.1016/j.micpath.2017.01.057

Cited by 12 publications

(13 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This nding clearly demonstrates that chicken-origin and non chicken-origin AAvV-1 isolates could infect chicken in a similar manner. In agreement to the present ndings, 100% mortality was recorded in chicken experimentally infected with peafowl-origin isolate [30]. In contrast, Ferreira et al [31] reported that the pigeon and cormorant lineage viruses required a high challenge dose to infect chickens and did not transmit e ciently to contact birds, even when a high infectious dose was used.…”

Section: Discussionsupporting

confidence: 89%

Simultaneous Detection of Velogenic Genotype XIII 2.2 Avian Avulavirus Type 1 (AAvV-1) From Spot-billed Pelican and Backyard Chicken: Implications to the Viral Maintenance and Spread

Balam¹,

Ratnamma²,

Isloor³

et al. 2021

Preprint

View full text Add to dashboard Cite

The present study demonstrates simultaneous isolation of genetically similar velogenic Avian Avula virus-1 from an apparently healthy spot- billed pelican and a naturally infected backyard chicken in the adjacent vicinity. A total of fourty eight cloacal swab samples from migratory birds (Painted storks, n=32 and Spot billed pelicans, n=16) at the Telineelapuram bird sanctuary in Andhra Pradesh, India and tissue samples of dead backyard chicken were collected. Two isolates were recovered, one each from a spot billed pelican (MIG-9) and a dead chicken (SKLM-1). The isolates were confirmed as velogenic based on mean death time, intra cerebral pathogenicity index and the putative fusion protein cleavage site (113R-R-K-R-F117). Phylogenetic analysis based on full- length fusion and attachment (HN) proteins classified the isolates into genotype XIII, sub- genotype 2.2. Sequence analysis revealed that the pelican and chicken isolates were 100% identical. The study isolates demonstrated multiple amino acid substitutions at several critical domains of F and HN proteins when compared with the current vaccine strains. Pathogenic and transmission potential of the AAvV-1 isolates was evaluated in three- week old chicken and the isolates proved to be highly virulent to chicken. To the best of our knowledge, this is the first evidence for the role of spot-billed pelicans in the maintenance of virulent AAvV-1 and its transmission to chicken. This study further highlights the role of wild birds in AAvV-1 transmission and the need for enhanced biosecurity in commercial poultry operations.

show abstract

Section: Discussionsupporting

confidence: 89%

Simultaneous Detection of Velogenic Genotype XIII 2.2 Avian Avulavirus Type 1 (AAvV-1) From Spot-billed Pelican and Backyard Chicken: Implications to the Viral Maintenance and Spread

Balam¹,

Ratnamma²,

Isloor³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The clinical infection was found to be more pronounced and severe between 2 nd to 4 th dpi with an exhibition of ocular and nasal discharge, conjunctivitis, ruffled feathers, greenish diarrhea and sudden death. Consistent to this observation, a similar pattern of clinical sign progression has previously been documented with an exposure of wild-bird originated velogenic strain in chicken [39,46]. Similarly, macroscopic lesion upon necropsy as well as microscopic changes observed under microscope were found suggestive of virulent AOAV-1 in a pattern similar to those reported previously during experimentally infected commercial chickens with wild bird originated velogenic isolates [37,39].…”

Section: Discussionsupporting

confidence: 89%

Evaluation of transmission potential and pathobiological characteristics of mallard originated Avian orthoavulavirus 1 (sub-genotype VII.2) in commercial broilers

Habib

Yaqub

Ul-Rahman

et al. 2019

Microbial Pathogenesis

View full text Add to dashboard Cite

Newcastle disease (ND), caused by Avian orthoavulavirus 1 (AOAV-1), affects multiple avian species around the globe. Frequent disease outbreaks are not uncommon even in vaccinates despite routine vaccination and, in this regards, viruses of diverse genotypes originating from natural reservoirs (migratory waterfowls) play an important role in a disease endemic setting. Though genomic characterization of waterfowl originated viruses has been will-elucidated previously, there is a paucity of data on clinico-pathological assessment of mallard-originated sub-genotype VII.2 in commercial chickens. Hence, the current study was designed to evaluate its transmission potential, tissue tropism and micro-and macroscopic lesions in commercial broilers. Based on complete genome and complete F gene, phylogenetic analysis clustered the study isolate within genotype VII and sub-genotype VII.2 in close association with those reported previously from multiple avian species worldwide. The study strain was found to be velogenic on the basis for typical residue pattern in the F-protein cleavage site (112 R-RQ-K-R↓F 117), sever disease induction in chicken, tissue tropism and subsequent clinico-pathological characteristics. Giving a clear evidence of horizontal transmission, a 100% mortality was observed by 4 th and 6 th day post infection (dpi) in chickens challenged with the virus and those kept with the challenged birds (contact birds), respectively. The observed clinical signs, particularly the greenish diarrhea, and macroscopic lesions such as pinpoint hemorrhages in proventriculus and cecal tonsils were typical of the infection caused by an AOAV-1 in chickens. The virus exhibited a broad tissue tropism where genomic RNA corresponding to study virus was detected in all of the tissues collected from recently mortile and necropsied birds. The study concludes that mallard-originated Avian orthoavulavirus 1 is highly velogenic to commercial chicken and, therefore, ascertain continuous vaccine monitoring in vaccinates and routine 3 surveillance of migratory/aquatic fowls to better elucidate infection epidemiology and subsequent potential impacts on commercial poultry.

show abstract

“…Whereas, cecal tonsils showed severely distended crypts containing high amount of necrotic debris associated with severe hemorrhage and lymphocytic infiltration including macrophages beside lymphocytic degeneration and necrosis (Fig.8). These results were supported by many previous reports that studied the effect of genotype VII NDV on tissues of immune system (Susta et al, 2014;Miller and Koch, 2013;Desingu et al, 2017 andDimitrov et al, 2019 b).…”

Section: Discussionsupporting

confidence: 89%

“…To detect the possible cause of nervous signs occurred after challenge with VV-NDV genotype VII, histopathological lesions were observed from brain tissue which recorded severe demyelination of brain tissue associated with severe gliosis and perivascular cuffing in addition to moderate to severe congestion of the BL.Vs (Fig. 8), as previously recorded by (Ecco et al, 2011;Koch, 2013 andDesingu et al, 2017).…”

Section: Discussionmentioning

confidence: 73%

Newcastle Disease Virus Vaccines Based On Genotype VII Strains Provide Efficient Protection Against Challenge With Circulating Very Virulent Field Virus (Genotype VII) In Broiler Chickens

Said

Sultan

2019

Journal of Current Veterinary Research

View full text Add to dashboard Cite

Newcastle disease virus (NDV) outbreaks still occur and cause disastrous economic losses in broiler chickens despite intensive vaccination policies using genetically different vaccines from the circulating strains. This study aimed to compare protection conferred by different vaccination strategies using genotype matched (genotype VII) and mismatched vaccine (genotype I and II) against challenge with currently circulating viruses in broiler chickens. 175 one-day-old broiler chicks were divided in to 5 groups. Groups 1 to 4 (G1, G2, G3 and G4) were vaccinated using genotype I (Ulster 2C strain), genotype II (VG/GA strain) and genotype VII (KBNP-4152R2L strain) vaccines according to manufacturer's instructions and group 5 (G5) was kept as control. Protection assessed based on clinical protection, seroconversion, shedding and histopathology. Chickens vaccinated with live genotype II vaccine at 7 and 21 days of age and killed genotype VII vaccine at 7 days of age (G2) were completely protected clinically with no mortality compared with control non vaccinated chickens (G5) that showed typical NDV clinical picture with (100%) mortality. while, other vaccinated groups showed lower level of clinical protection with (2.8%) mortality for each group. Viral shedding was greatly reduced in groups vaccinated with inactivated recombinant genotype VII vaccine (G2 and G3) which recorded (0% & 0%) and (16.6% & 0%) at 7 & 10 days post challenge, respectively compared with groups vaccinated with inactivated genotype II vaccine (G1 and G4) regardless the genotype of the combined live vaccine (either genotype II or VII). Moreover, histopathology of control non vaccinated challenged group (G5) revealed severe lymphocytic depletion and necrosis, epithelial degeneration beside neurological lesions. It was concluded that using genotype matched NDV vaccine with the currently circulating field strains can provide adequate clinical protection and minimize virus shedding that can help in decreasing virus load in the environment.

show abstract

Clinicopathological characterization of experimental infection in chickens with sub-genotype VIIi Newcastle disease virus isolated from peafowl

Cited by 12 publications

References 19 publications

Simultaneous Detection of Velogenic Genotype XIII 2.2 Avian Avulavirus Type 1 (AAvV-1) From Spot-billed Pelican and Backyard Chicken: Implications to the Viral Maintenance and Spread

Simultaneous Detection of Velogenic Genotype XIII 2.2 Avian Avulavirus Type 1 (AAvV-1) From Spot-billed Pelican and Backyard Chicken: Implications to the Viral Maintenance and Spread

Evaluation of transmission potential and pathobiological characteristics of mallard originated Avian orthoavulavirus 1 (sub-genotype VII.2) in commercial broilers

Newcastle Disease Virus Vaccines Based On Genotype VII Strains Provide Efficient Protection Against Challenge With Circulating Very Virulent Field Virus (Genotype VII) In Broiler Chickens

Contact Info

Product

Resources

About