Clinicopathological and molecular characteristics of synchronous gastric adenocarcinoma and gastrointestinal stromal tumors

Luo, Jun; Cao, Fa-Long; Meng, Chen; Lin, Lijun; Ma, Sisi; Peng, Shaohua; Gao, Hongling; Javidiparsijani, Sara; Wang, Guirong; Zhang, Menglan; Xin, Jianguo; Wang, Yichun; Zhang, Shukun

doi:10.1038/s41598-017-12622-x

Cited by 12 publications

(18 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tumor cells have the characteristics of malignant proliferation, migration and invasion ( 24 ) which significantly influence the development of cancer. Therefore, identifying specific genetic markers associated with tumor growth and metastasis may be useful to study cancer progression.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of RPN2 induces apoptosis and inhibits migration and invasion in colon carcinoma

Jiang

2018

Oncol Rep

View full text Add to dashboard Cite

The morbidity of colorectal cancer (CRC) increases annualy, which accounts to higher mortality worldwide. Therefore, it is important to study the pathogenesis of colon cancer. Ribophorin II (RPN2), part of the N-oligosaccharyltransferase complex, is highly expressed in CRC. In the present study, we investigated whether RPN2 can regulate apoptosis, migration and invasion by RNA interference in CRC and sought to clarify the molecular mechanism involved. Based on previous research, an abnormal high expression of RPN2 was observed in CRC tissues and cell lines by real-time (RT)-PCR, immunohistochemistry (IHC) and western blot analysis. RPN2 knockdown via small RNA interference (siRNA) strategy attenuated the expression of RPN2 at the mRNA and protein levels in vivo, leading to decreased cell viability and increased cell apoptosis. In addition, RNAi-RPN2 effectively arrested the cell cycle at the G0/G1-phase in SW1116 and SW480 cells. Furthermore, the Transwell assay demonstrated that cell migration and invasion abilities were significantly inhibited after cell transfection with RPN2 interference plasmid. The apoptosis-related protein (caspase-3) expression was increased and the cell cycle-related protein (cyclin D1) expression was decreased in the siRNA-RPN2 group. RT-PCR and western blot analysis results indicated that migration- and invasion-related proteins including E-cadherin, matrix metalloproteinases (MMP)-2 and TIMP-2 were markedly regulated by RPN2 siRNA. Phosphorylation levels of signal transducer and activator of transcription (STAT)3 and Janus kinase (JAK)2 were inhibited by RPN2 siRNA. These findings indicated a novel pathway of tumor-promoting activity by RPN2 in CRC, with significant implications for unraveling the tumorigenesis of CRC.

show abstract

Section: Discussionmentioning

confidence: 99%

Downregulation of RPN2 induces apoptosis and inhibits migration and invasion in colon carcinoma

Jiang

2018

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…Adenocarcinoma is the most common malignant stomach tumor, while GIST is a stromal tumor of the digestive tract that occurs in less than 1% of all gastrointestinal malignancies [7]. Synchronous tumors are not a very common tumor association, and are usually detected only during the histopathological evaluation [8]. When the GIST is submucosal or subserosal, the gastric mucosa may not be invaded and the endoscopic biopsies can be normal.…”

Section: Discussionmentioning

confidence: 99%

Synchronous adenocarcinoma and gastrointestinal stromal tumor in the stomach - report of two cases

et al. 2019

View full text Add to dashboard Cite

Introduction Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor that occurs in the gastrointestinal tract, most commonly in the stomach or the small intestine. The surgery of the stomach is the dominant way of treatment of these tumors. The synchronous detection of adenocarcinoma and gastric GIST is not a very common condition, which is often diagnosed intraoperatively and has a significant impact on the prognosis of these patients. Outline of cases We herein report two cases of gastric GIST with synchronous adenocarcinoma tumors, which were detected incidentally, intraoperatively, while the patients were undergoing surgery for a primary gastric adenocarcinoma. The first case was of a 76-year-old female patient. The histopathological analysis of the operative specimen firstly showed a poorly differentiated advanced gastric adenocarcinoma. The second tumor, from the gastric serosa, was a spindle cell GIST of low risk. It was diffusely positive for DOG1, CD34, and CD117. Its proliferative index was established using the Ki67 antibody. The number of mitoses was one per 5 mm 2. The second case was of a 65-year-old male patient. The histopathological analysis revealed an early, well-differentiated, intestinal type adenocarcinoma of the gastric mucosa. The synchronous tumor from the serosa of the stomach was a spindle cell gastrointestinal stromal tumor (CD34, DOG1, and CD117 diffusely positive) of low risk. The proliferative index of this tumor, labeled with the Ki67 antibody, was very low. Necrosis was not present, nor was mitosis. Conclusion Synchronous adenocarcinomas and GIST of the stomach are not very commonly associated, and are usually detected intraoperatively and after an immunohistochemical analysis. Recognition of this condition has a very important role in a differential diagnosis and the exclusion of metastases of malignant tumor deposits. Based on the tumor severity, the radicalness of the surgical intervention is determined, which affects the outcome of these patients.

show abstract

“…GISTs may be detected as an isolated pathology or may be synchronous with another neoplastic lesion, such as gastric adenocarcinoma [18], rectal carcinoma [3], colonic carcinoma [9] or neuroendocrine tumour of the pancreas [19]. The co--existence of multiple malignancies in the gut from different primary origins raises the question of a possible common carcinogenesis pathway.…”

Section: Discussionmentioning

confidence: 99%

“…This marker shows expression in early hematopoietic and vascular-associated tissue. CD34 antigen expression has been reported to be positive in more than 91% of GISTs [18,34,36,37]. It is also present in Pseudo-Angiomatous Stromal Hyperplasia of Breast (PASH) [38] but negative in typical leiomyoma [36].…”

Section: Discussionmentioning

confidence: 99%

Incidental micro-GIST found on post-sleeve gastrectomy. A case report and literature review

Al‐Zawi¹,

Salama²,

Ho³

et al. 2019

Nowotwory. Journal of Oncology

View full text Add to dashboard Cite

Gastrointestinal stromal tumours (GIST) are the most common type of primary mesenchymal tumour within the wall of the gastrointestinal tract. They are usually derived from a mutation of the KIT or PDGFRA genes. The most common sites of GISTs are the stomach and small intestine. Presentation may include local symptoms, such as nausea and vomiting, GI bleeding and abdominal pain. Approximately 25% of GISTs are found incidentally during radiological investigations and 0.7% of resected specimens in bariatric procedures show an incidental GIST. Here we report a case of an incidental micro-GIST found on a post-sleeve gastrectomy specimen.

show abstract

Clinicopathological and molecular characteristics of synchronous gastric adenocarcinoma and gastrointestinal stromal tumors

Cited by 12 publications

References 44 publications

Downregulation of RPN2 induces apoptosis and inhibits migration and invasion in colon carcinoma

Downregulation of RPN2 induces apoptosis and inhibits migration and invasion in colon carcinoma

Synchronous adenocarcinoma and gastrointestinal stromal tumor in the stomach - report of two cases

Incidental micro-GIST found on post-sleeve gastrectomy. A case report and literature review

Contact Info

Product

Resources

About