Clinicopathologic significance of tumor cell-lined vessel and microenvironment in oral squamous cell carcinoma

Liu, Shyun Yeu; Chang, Long Chang; Li, Pan; Hung, Yi Jen; Lee, Chin Hai; Shieh, Yi‐Shing

doi:10.1016/j.oraloncology.2007.02.007

Cited by 85 publications

(73 citation statements)

References 27 publications

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“…Accumulation of TAMs in the cancer stroma is correlated with angiogenesis in various cancers (33)(34)(35)(36)(37), including oral cancer. TAMs also increase the secretion of angiogenic factors such as vascular endothelial growth factor and contribute to the integrity of the vascular structure of OSCC (8,38). However, contradictory data has also been reported indicating a lack of correlation between TAM distribution and angiogenic activity (39).…”

Section: Discussionmentioning

confidence: 99%

Comparison of MMP2 and MMP9 expression levels between primary and metastatic regions of oral squamous cell carcinoma

et al. 2016

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Matrix metalloproteinases (MMPs)and tumor-associated macrophages (TAMs) play important roles in tumor growth. The present study investigated the expression levels of MMP2 and MMP9 in relation to the distribution of TAMs in the primary and metastatic regions of oral squamous cell carcinoma. Twenty-nine cases of oral squamous cell carcinoma (OSCC) with regional lymph node metastasis were selected from available documents in the archives of the Department of Pathology, Nihon University School of Dentistry. Four-micrometerthick sections were prepared from the primary and metastatic regions. Each section was subjected to immunohistochemical staining using anti-MMP2, anti-MMP9, and anti-CD68 antibodies. The distribution and localization of MMPs and TAMs were compared between primary and metastatic regions. The expression levels of both MMPs were higher in the metastatic regions of lingual and gingival cancers. Statistically significant differences were observed in both T1 and T2 cases. In contrast to the higher expression of MMPs in metastatic regions, a higher number of TAMs were distributed in the primary regions. From these results, MMP expression levels and the numbers of TAMs were expected to have an inverse relationship between the primary and metastatic regions of OSCC. (J Oral Sci 58, 59-65, 2016)

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Section: Discussionmentioning

confidence: 99%

Comparison of MMP2 and MMP9 expression levels between primary and metastatic regions of oral squamous cell carcinoma

et al. 2016

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“…HIF-1a expression in oral squamous cell carcinoma (OSCC) has been shown in several recent studies (11,13,(18)(19)(20)(21). HIF-1a expression has been shown to correlate with poor prognosis and is an aggressive index of OSCC (19,22,23). Although both 18 F-FMISO PET and HIF-1a are important factors for hypoxia evaluation in cancer, there have been no previous reports regarding the correlation between 18 F-FMISO PET findings and HIF-1a expression in patients with OSCC.…”

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confidence: 99%

¹⁸F-Fluoromisonidazole PET Uptake Is Correlated with Hypoxia-Inducible Factor-1α Expression in Oral Squamous Cell Carcinoma

et al. 2013

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Hypoxia is a common feature of cancer and a prognostic factor for many types of cancer. 18 F-fluoromisonidazole ( 18 F-FMISO) PET can detect tumor hypoxia noninvasively. Hypoxia-inducible factor-1 (HIF-1) is a key player in the transcriptional response to low oxygen tension in many types of cancer. Its activity is mainly dependent on the stability and modification of HIF-1a, which is a composition of HIF-1. However, it is unclear whether 18 F-FMISO PET can identify HIF-1a expression in oral squamous cell carcinoma (OSCC). The present study was performed to elucidate the correlation between 18 F-FMISO PET findings and HIF-1a expression in OSCC. Methods: Twenty-three patients (age range, 42-84 y; 15 men, 8 women) with OSCC were enrolled in this study. The T-stages of cancer were T1 in 1 patient, T2 in 9, T3 in 2, and T4a in 11. The N-stages were N0 in 13 patients, N1 in 5, and N2 in 5. Each patient underwent 18 F-FMISO and 18 F-FDG PET before surgery, and the maximum standardized uptake value (SUV max ) of both PET studies was measured. HIF-1a expression in the operation materials was evaluated by immunohistochemical staining. The SUV max of both PET studies and HIF-1a findings were compared statistically. Results: 18 F-FMISO PET detected uptake in the primary site in 14 of the 23 patients (61%). The median SUV max of 18 F-FMISO and 18 F-FDG PET in the primary site was 1.83 (range, 0.8-2.7) and 16.5 (range, 1.0-32.3), respectively. There was a weak significant correlation between 18 F-FMISO and 18 F-FDG PET SUV max (P 5 0.02, r 5 0.48). HIF-1a expression was clearly detected in 11 of the 23 patients (48%). The 18 F-FMISO PET SUV max was significantly higher in the HIF-1a-positive cases than in the HIF-1a-negative cases (median, 2.1; range, 1.5-2.4, vs. median, 1.6; range, 0.8-2.0, respectively) (P 5 0.002). However, there were no significant correlations between 18 F-FDG PET SUV max and HIF-1a expression (median, 21.8; range, 7.7-29.1 vs. 1.0-32.2) (P 5 0.06). Conclusion: 18 F-FMISO uptake in the primary site of OSCC indicates a hypoxic environment with HIF-1a expression.

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“…The infiltrating macrophage count was correlated with the progression of OSCC and is a prognostic marker [58,59]. Cytomorphological alterations indicate the pathological status of the concerned cell.…”

Section: Discussionmentioning

confidence: 96%

Nuclear pleomorphism-based cytopathological grading in human oral neoplasm

Mohanta¹,

Prafulla²

2017

Russ. Open Med. J.

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Objective -The objective of the present study is record the nuclear pleomorphism in various stages of oral carcinogenesis and to analyse their utility in cytopathological grading for early detection of human oral cancer Material and Methods -In this hospital based case-control study, oral site, age-group and sex-matched 272 subjects (136 cases and 136 normal healthy individuals) were included. Scraped exfoliated cytosmears were collected from the affected oral site of the subjects and smearing was done in the pre-cleaned-coded glass-slides. Two such slides were prepared from each subject. The cytosmears were immediately fixed in aceto-alcohol (1 part of glacial acetic acid: 3 part of absolute ethyl alcohol) fixative. One set of the slide was stained with Papanicolaou's stain and the other set was counter-stained with Giemsa's Solution for cytopathological analysis. Test of proportion (ztest) was followed and the critical ratio (z-value) was calculated for the test of significance. Results -Nuclear pleomorphism in the form of round, oval, spindle, elongated fiber as well as irregular shapes were mostly observed in oral squamous cells during different stages of carcinogenesis. Appearance of such nuclear pleomorphism in human oral neoplasm may be considered as a sign of cellular alternation in general and index of oral carcinogenesis in particular. In the present study, the frank malignant cases mimic to be either premalignant lesions or benign/carcinoma in situ were detected on the basis of nuclear pleomorphismbased cytopathological grading and so an increasing trend was observed from precancerous lesions to malignant cases due to shifting of numbers. Diagnostic tests also indicated that the Sensitivity was calculated to be 83.5%, Specificity was 100%, positive predictive value (PPV) was 100%, negative predictive value (NPV) was 30% and the accuracy was found to be 84.6%. Therefore, the nuclear pleomorphismbased cytopathological grading system makes itself an ideal screening test for early detection of human oral cancer. Conclusion -Pattern of nuclear pleomorphism corresponding to various cytological atypias is a common feature observed during different stages of oral carcinogenesis and thus, it has a practical implication in grading and early detection of oral cancer.

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Clinicopathologic significance of tumor cell-lined vessel and microenvironment in oral squamous cell carcinoma

Cited by 85 publications

References 27 publications

Comparison of MMP2 and MMP9 expression levels between primary and metastatic regions of oral squamous cell carcinoma

Comparison of MMP2 and MMP9 expression levels between primary and metastatic regions of oral squamous cell carcinoma

¹⁸F-Fluoromisonidazole PET Uptake Is Correlated with Hypoxia-Inducible Factor-1α Expression in Oral Squamous Cell Carcinoma

Nuclear pleomorphism-based cytopathological grading in human oral neoplasm

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Clinicopathologic significance of tumor cell-lined vessel and microenvironment in oral squamous cell carcinoma

Cited by 85 publications

References 27 publications

Comparison of MMP2 and MMP9 expression levels between primary and metastatic regions of oral squamous cell carcinoma

Comparison of MMP2 and MMP9 expression levels between primary and metastatic regions of oral squamous cell carcinoma

18F-Fluoromisonidazole PET Uptake Is Correlated with Hypoxia-Inducible Factor-1α Expression in Oral Squamous Cell Carcinoma

Nuclear pleomorphism-based cytopathological grading in human oral neoplasm

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¹⁸F-Fluoromisonidazole PET Uptake Is Correlated with Hypoxia-Inducible Factor-1α Expression in Oral Squamous Cell Carcinoma