Clinicopathologic Features of Breast Carcinomas Classified by Biomarkers and Correlation with Microvessel Density and VEGF Expression: A Study from Thailand

Chuangsuwanich, Tuenjai; Pongpruttipan, Tawatchai; O-charoenrat, Pornchai; Komoltri, Chulaluk; Watcharahirun, Suwapee; Sa-nguanraksa, Doonyapat

doi:10.7314/apjcp.2014.15.3.1187

Cited by 14 publications

(8 citation statements)

References 23 publications

(49 reference statements)

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“…(Clark et al, 1987;Nilsson et al, 2001;Hooshmand et al, 2014). Quantification of ERα mRNA and protein expression in breast cancer tissues using real time PCR assay and immunohistochemistry has been previous reported (Bieche et al, 2001;De Cremoux et al, 2002;Chuangsuwanich et al, 2014;Wang et al, 2014). In this study, we demonstrated altered ERα mRNA and protein expression for the first time in Iranian population.…”

Section: Discussionsupporting

confidence: 62%

Estrogen Receptor Alpha Gene Expression in Breast Cancer Tissues from the Iranian Population - a Pilot Study

Hosseini¹,

Gopalan²,

Nassiri³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Estrogen receptor alpha (ERα) is one of the major sub-types of estrogen receptors. ERα plays an important role in cellular proliferation and differentiation, chiefly in mammary tissues. In the present study we aimed to quantify of ERα mRNA and protein expression in breast tissues from the Iranian population using a real-time PCR assay. Twenty nine breast tissues including 19 adenocarcinomas and 10 normal controls were recruited from the Iranian population. mRNA extraction and cDNA synthesis were performed from these tissues using commercial kits. ERα mRNA and protein expression was quantified using real-time PCR and immunohistochemistry respectively. The results showed high expression of ERα mRNA (68%) and protein (53%) in the majority of breast cancer tissues compared to normal breast tissues (p= 0.035). Also, high ERα mRNA was associated with tumour size of breast carcinomas. In this study, we first reported the expression of ERα in Iranian patients with breast cancers and demonstrated prevalence of the expression to be similar to breast cancers noted in other populations.

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Section: Discussionsupporting

confidence: 62%

Estrogen Receptor Alpha Gene Expression in Breast Cancer Tissues from the Iranian Population - a Pilot Study

Hosseini¹,

Gopalan²,

Nassiri³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Suggesting that worse short-term survival for HR-/HER2+ (HER2-enriched) and triplenegative breast cancers is consistent with prior studies that had 5 or 10 years of follow up. Earlier studies in Thailand TNs demonstrated significantly higher tumor grade, mitotic count, Ki-67 index, p53 and vimentin and decreased overall survival compared with nonTN (Chuangsuwanich et al, 2014). HER-2 and basal-like subtypes were likely to have high tumor grade, HER-2 subtype larger, had higher number of nodal involvement (Chuthapisith et al, 2012).…”

Section: Discussionmentioning

confidence: 89%

Molecular Subtypes and Prognostic Factors among Premenopausal and Postmenopausal Thai Women with Invasive Breast Cancer: 15 Years Follow-up Data

Tubtimhin

Promthet

Suwanrungruang

et al. 2018

Asian Pac J Cancer Prev

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Background and Purpose:This study focused on molecular subtypes and prognostic factors for survival of pre- and post-menopausal breast cancer patients.Methods:A retrospective cohort study was performed on 523 patients with invasive carcinoma of the breast treated at Ubon Ratchathani Cancer Hospital, Thailand from 2002 to 2016. Patient characteristics were collected based on a systematic chart audit from medical records. Prognostic factors were performed by observe survival analysis. A Cox regression model was used to calculate hazard ratios of death, taking into account the age and menopause status, molecular subtype, stage of disease, histological grade, lymphatic and vascular invasion, resection margin, hormone receptor expression, and treatment modality.Results:The median time from the diagnosis of invasive breast cancer to the last follow-up or death was 10.2 [95% CI = 9.28-11.95] years in premenopausal women, and 7.4 [95% CI = 6.48-8.44] years in postmenopausal cases. The overall survival estimates at 5 and 10 years for younger woman of 71.2% and 51.8% respectively, appeared slightly better than the 68.3% and 40.9% for postmenopausal women [HRadj = 1.27, 95% CI =0.99-1.63]. In the multivariate analysis, 3 prognostic indicators significantly predicted a worse overall survival in premenopausal patients, triple negative subtype [HRadj = 6.03, 95% CI = 1.94-18.74], HER2-enriched status [HRadj = 4.11, 95% CI = 1.59-10.65] and stage III [HRadj = 2.73, 95% CI = 1.10-6.79]. Statistically significant increased risk of death in postmenopausal patients was noted for only chemotherapy after mastectomy [HRadj = 8.76, 95% CI = 2.88-26.61], and for a Luminal B status [HRadj = 3.55, 95% CI = 1.47-8.53].Conclusion:Postmenopausal women with invasive breast cancer experience a significantly shorter survival than do their premenopausal counterparts. The predictors of worse overall survival were molecular subtype, stage of disease and type of treatment administered.

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“…We examined potential mechanisms of action of MTDH/AEG-1 in BUC by exploring the relationship between the expression of MTDH/AEG-1 and that of seven other common biomarkers: caspase-3, Bcl-2, VEGF, Ki-67, PCNA, p53, and DcR3. Sequential activation of caspase-3 plays a central role in cell apoptosis,44 the Bcl-2 gene can inhibit tumor cell apoptosis,45–47 and VEGF production by cells can stimulate vasculogenesis and angiogenesis 48,49. Ki-67 and PCNA are classical markers of cell proliferation and are routinely used by pathologists,50 whereas p53 is related to DNA repair and apoptosis 47,51.…”

Section: Discussionmentioning

confidence: 99%

Clinical significance and effect of MTDH/AEG-1 in bladder urothelial cancer: a study based on immunohistochemistry, RNA-seq, and in vitro verification

Zhang

Dang

et al. 2018

CMAR

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BackgroundOverexpression of metadherin/astrocyte elevated gene-1 (MTDH/AEG-1) has been implicated in various cancers. However, the clinical significance and the potential biological functions of MTDH/AEG-1 in bladder urothelial carcinoma (BUC) are not established.MethodsIn this study, the expression of MTDH/AEG-1in BUC was measured using the Cancer Genome Atlas (TCGA) database and immunohistochemistry, together with a meta-analysis, to investigate the expression and diagnostic value of MTDH/AEG-1. The possible association between MTDH/AEG-1 expression and the viability, proliferation, and apoptosis in BUC cell lines (T24, HT1376, and RT4) was also assessed in vitro by viability, MTS, colony formation, and caspase-3/7 assays, as well as Hoechst 33342 and propidium iodide (PI) double staining.ResultsMTDH/AEG-1 expression was significantly higher in BUC tissues than in normal bladder tissues, according to the TCGA and immunohistochemistry results, and these findings were verified by the meta-analysis. Functional knockdown of MTDH/AEG-1 suppressed BUC cell growth and induced apoptosis. Bioinformatics analyses indicated an involvement of MTDH/AEG-1 in several processes, including RNA binding, protein transport, intracellular transport, and the insulin signaling pathway.ConclusionWe hypothesize that MTDH/AEG-1 could play essential roles in BUC, especially in cell growth and apoptosis, via the insulin signaling pathway.”

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Clinicopathologic Features of Breast Carcinomas Classified by Biomarkers and Correlation with Microvessel Density and VEGF Expression: A Study from Thailand

Cited by 14 publications

References 23 publications

Estrogen Receptor Alpha Gene Expression in Breast Cancer Tissues from the Iranian Population - a Pilot Study

Estrogen Receptor Alpha Gene Expression in Breast Cancer Tissues from the Iranian Population - a Pilot Study

Molecular Subtypes and Prognostic Factors among Premenopausal and Postmenopausal Thai Women with Invasive Breast Cancer: 15 Years Follow-up Data

Clinical significance and effect of MTDH/AEG-1 in bladder urothelial cancer: a study based on immunohistochemistry, RNA-seq, and in vitro verification

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