Clinicopathologic Evaluation of Hepatic Lipidosis in Periparturient Dairy Cattle

Kalaitzakis, Emmanouil; Roubies, N.; Panousis, Nikolaos; Pourliotis, Konstantinos; Kaldrymidou, E.; Karatzias, H.

doi:10.1111/j.1939-1676.2007.tb03029.x

Cited by 46 publications

(32 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liver concentration of triglycerides (Tables 5 and 6) was low, according with levels reported by other authors (Kalaitzakis et al, 2007;Starke et al, 2010;Gross et al, 2013). Supplementation with 200 g/d L-carnitine fumarate significantly decreased hepatic triglyceride concentrations (Table 5), possibly due to a higher rate of fatty acid oxidation by the liver, decreasing their availability for esterification.…”

Section: Discussionsupporting

confidence: 80%

L-carnitine supplementation descreases hepatic triglyceride accumulation in Holstein cows during the transition period

Galvis

Madrid

Vásquez

2019

Rev Colom Cienc Pecua

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 80%

L-carnitine supplementation descreases hepatic triglyceride accumulation in Holstein cows during the transition period

Galvis

Madrid

Vásquez

2019

Rev Colom Cienc Pecua

View full text Add to dashboard Cite

show abstract

“…The diagnosis of hepatic lipidosis was obtained by liver biopsy and histologic inspection. The stage of disease was classified as described [15] according to the different extension of lipid deposition across the morphologic liver zones (periportal, transition zone and pericentral): group 1 (no lipid deposition), group 2 (only one zone affected), group 3 (all three zones affected), group 4 (Kupffer cells affected in addition to all three zones). Ethics approval was not requested and not needed because samples were taken from diseased animals during routine diagnostic investigations when hepatic lipidosis was suspected in peripartal dairy cows.…”

Section: Methodsmentioning

confidence: 99%

Metabolomic biomarkers correlating with hepatic lipidosis in dairy cows

et al. 2014

View full text Add to dashboard Cite

BackgroundHepatic lipidosis or fatty liver disease is a major metabolic disorder of high-producing dairy cows that compromises animal performance and, hence, causes heavy economic losses worldwide. This syndrome, occurring during the critical transition from gestation to early lactation, leads to an impaired health status, decreased milk yield, reduced fertility and shortened lifetime. Because the prevailing clinical chemistry parameters indicate advanced liver damage independently of the underlying disease, currently, hepatic lipidosis can only be ascertained by liver biopsy. We hypothesized that the condition of fatty liver disease may be accompanied by an altered profile of endogenous metabolites in the blood of affected animals.ResultsTo identify potential small-molecule biomarkers as a novel diagnostic alternative, the serum samples of diseased dairy cows were subjected to a targeted metabolomics screen by triple quadrupole mass spectrometry. A subsequent multivariate test involving principal component and linear discriminant analyses yielded 29 metabolites (amino acids, phosphatidylcholines and sphingomyelines) that, in conjunction, were able to distinguish between dairy cows with no hepatic lipidosis and those displaying different stages of the disorder.ConclusionsThis proof-of-concept study indicates that metabolomic profiles, including both amino acids and lipids, distinguish hepatic lipidosis from other peripartal disorders and, hence, provide a promising new tool for the diagnosis of hepatic lipidosis. By generating insights into the molecular pathogenesis of hepatic lipidosis, metabolomics studies may also facilitate the prevention of this syndrome.

show abstract

“…Blood serum total protein, albumin and urea concentrations represent not just indicators of protein metabolism (Kida, 2002), but also liver function biomarkers (Kaneko, 1989). A more specific liver function biomarker in the blood serum is AST activity (Kaneko, 1989;Garry et al, 1994;Rehage et al, 1996;Kida, 2002) and among most commonly used hepatic status indicators is total blood serum bilirubin concentration (Rehage et al, 1996;Kalaitzakis et al, 2007). Blood serum glucose concentration is an energy metabolism indicator, but it can also be used as a liver function biomarker, since hepatic gluconeogenesis is the major contributor to the blood glucose homeostasis in dairy cows (Kida, 2002).…”

Section: Introductionmentioning

confidence: 99%

Endocrine and metabolic profile in Holstein and red Holstein heifers during peripartal period

Kasagic¹,

Radojicić²,

Gvozdić³

et al. 2011

Acta vet. (Beogr.)

View full text Add to dashboard Cite

The aim of this stady was to examine the relationship between triiodothyronine (T3), thyroxin (T4), insulin-like growth factor-I (IGF-I) and blood serum liver function biomarkers concentrations in Holstein and Red Holstein heifers during the peripartal period. In the first experimental group (I) were heifers of Holstein Friesian breed (n=10), and in the second experimental group (II) were Red Holstein heifers (n=10). Blood samples were taken from the vena coccygea three times during the short peripartal period (5 days before delivery, at delivery and 5 days after delivery), and blood serum samples were separated after spontaneous coagulation. Blood serum T3, T4 and IGF-I concentrations were determinated using radioimmunoassay (RIA). Blood serum concentrations of glucose (G), total protein (TP), albumin, (Alb), urea, total bilirubin (tBil) and aspartate aminotrasferase (AST) were determined using commercial ELISA kits. The statistical significance between means was determined using Student t-test. Blood serum T3 concentration in Group II was significantly lower after delivery, and T4 concentration was significantly higher before delivery (p<0.05), compared to Group I. Mean blood serum IGF-I concentrations between the two experimental groups were not significantly different at all time intervals. Relationships between the determined hormones showed a median positive correlation between T3, and T4 (r=0.74) in Group I and highly positive correlation in Group II (r=0.81). Blood serum thyroid gland hormones and IGF-I concentrations in healthy heifers during peripartal period vary considerably, the lowest values being determined immediately after calving in both experimental groups. Blood serum G, TP, Alb and urea concentrations were significantly higher in Group I at all investigated time intervals. Blood serum tBil concentration was significantly higher after partus in Group I (11.02 ± 3.33 : 8.49 ± 3.35 μmol/L, Group I vs. II, respectively), and AST activity was not significantly different at all intervals

show abstract

Clinicopathologic Evaluation of Hepatic Lipidosis in Periparturient Dairy Cattle

Abstract: Except for OCT, AST, and tBIL, none of the biochemical tests used, including SBA, had sufficient discriminatory power to differentiate reliably between mild and severe FCL because of poor sensitivity. A weak correlation between clinical signs and the extent of FCL was evident.

Cited by 46 publications

References 42 publications

L-carnitine supplementation descreases hepatic triglyceride accumulation in Holstein cows during the transition period

L-carnitine supplementation descreases hepatic triglyceride accumulation in Holstein cows during the transition period

Metabolomic biomarkers correlating with hepatic lipidosis in dairy cows

Endocrine and metabolic profile in Holstein and red Holstein heifers during peripartal period

Contact Info

Product

Resources

About