Clinico-mycological study of onychomycosis in children from a tertiary care center

Jeelani, Shazia; Lanker, Audil Mohmad; Jeelani, Nasir; Ahmed, Qazi Masood; Fazili, Tawhida; Bashir, Humera

doi:10.4103/2319-7250.179411

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“…In the present study, using KOH wet mount, 64% of samples from patients with onychomycosis and 68% of those from tinea capitis were positive for fungal elements (hyphae or spores). These results are consistent with other studies,8,9 that reported rates of positivity of 62% and 66%, respectively, for fungal elements (hyphae and/or spores) in patients with onychomycosis.…”

Section: Discussionsupporting

confidence: 93%

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<p>Evaluation Of The Efficacy Of Fluorescent Staining And Chicago Sky Blue Staining As Methods For Diagnosis Of Dermatophytosis In Hair And Nails</p>

Mourad

Ismail

Hawwam

et al. 2019

CCID

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PurposeDermatophytes are fungi that cause infections affecting hair, nail, and skin; in nails they cause onychomycosis, while in hair they lead to tinea capitis. Detection of dermatophytes using traditional methods, including potassium hydroxide (KOH) and culture on agar-based media leads to high rates of false-negative results. Here, we investigated more accurate diagnostic techniques, including Chicago sky blue staining and Calcofluor white fluorescent staining and compared them with traditional KOH and culture methods for the diagnosis of fungi causing onychomycosis and tinea capitis.Patients and methodsThis study was conducted using samples from 50 patients with dermatophytosis of the hair or nail. Samples were subjected to all the following laboratory investigations: KOH wet mount, culture on Sabouraud’s dextrose agar, Chicago sky blue staining, and Calcofluor white staining. The results of the new diagnostic techniques were compared with those of the traditional methods.ResultsCalcofluor white stain and Chicago sky blue stain for dermatophytosis of hair and nail are more specific and sensitive as compared to traditional diagnostic methods. KOH wet mount is simple, rapid, and inexpensive test but lacks color contrast and gave more false positive (artifacts) and false-negative results as compared to these new stain methods.ConclusionChicago sky blue and Calcofluor white staining are excellent methods for diagnosis of fungal infections, including those that cannot be confirmed using conventional methods.

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Section: Discussionsupporting

confidence: 93%

“…Dass et al (2015)7 reported that, although culture is a good method for definite diagnosis of dermatophytes, it generates numerous false-negative results, with positive results in only (39%), while Jeelani et al (2016)8 reported that culture generated positive results in 58% of dermatophytes in onychomycosis.…”

Section: Discussionmentioning

confidence: 99%