Clinico-hematological and tissue changes induced by butachlor in male Japanese quail (Coturnix japonica)

Hussain, Riaz; Khan, Ahrar; Mahmood, Fazal; Sarmad, Rehan; Ali, Farah

doi:10.1016/j.pestbp.2014.01.005

Cited by 44 publications

(26 citation statements)

References 23 publications

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“…Earlier studies have indicated that nuclear alterations in erythrocytes could be due to chromosomal abnormalities induced by variety of toxic substances (Hussain et al, 2012). The lobed and blebbed nuclei erythrocyte in present study could be due to failure of tubulin polymerization, oxidation of mRNA and nitration of proteins which have impact on intracellular metabolism (Campos-Pereira et al, 2012;Hussain et al, 2014). The concentration of different serum enzymes including ALT, AST and ALP increased significantly in insecticide exposed fish.…”

Section: ×1000 Giemsa Stainmentioning

confidence: 45%

“…Alterations in different hematological parameters and blood chemistry of fish exposed to different pollutants can be used as the most suitable and reliable biomarkers to determine the physiological indices of stress in fish induced by various toxic substances (Karanthi et al, 2004;Hussain et al, 2014). The decreased values of erythrocyte could be due to toxic impacts of insecticide on blood producing tissues and on circulating erythrocytes.…”

Section: ×1000 Giemsa Stainmentioning

confidence: 99%

“…In addition, occupational exposure to pesticides poses major threats including adverse reproduction, congenital anomalies and genotoxic effects (Hussain et al, 2011;Ahmad et al, 2012). Due to less toxic and low persistence than many other compounds these pesticides are routinely employed in the field of agriculture, livestock sector, public health management and medicine (Deka et al, 2012;Hundekari et al, 2013;Hussain et al, 2014). Frequent and extensive use of organophosphorus insecticides results in accumulation of these chemicals in natural water systems and variety of daily consumable food materials including vegetables and grains as a the major sources of contact (Schipper et al, 2008;Edwards et al, 2013;Rivadeneira et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, irreversible changes in DNA can result in anxiety during cell division ultimately leading to aneuploidy and variations in nuclear DNA contents. In addition the blood cells have been shown to reveal all the physical and chemical changes when exposed to various (Akcha et al, 2012;Lewis and Ford, 2012;Hussain et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Hemato-biochemical and Genetic Damage Caused by Triazophos in Fresh Water Fish, Labeo rohita

Ghaffar¹,

Hussain²,

Khan³

et al. 2015

IJAB

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Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and poses serious threats to both environment and aquatic organisms. In the present study micronucleus assay and nuclear abnormalities were used as a biomarker to assess the cyto-genotoxic potential of different concentrations of triazophos insecticide in fresh water fish (Labeo rohita L.). For this purpose 16 fresh water fish of same age and weight were kept into four equal groups (A-D) having four each. All the fish were kept in aquaria with 75 L water capacity for six days for acclimatization. Various sub-lethal concentrations of organophosphate insecticide triazophos (0.010, 0.015 and 0.200 ppm) were tested. For hematological and cyto-genotoxic studies blood samples were collected from each fish after 48, 72 and 96 h of post-treatment. Duplicate thin blood smear was made from fresh blood of each fish. Results revealed that total erythrocyte count, pack cell volume, hemoglobin concentration, serum total proteins, mean corpuscular hemoglobin concentration and mean corpuscular volume values were significantly (P<0.05) decreased, which indicated that fish were suffering from microcytic hypochromic anemia. Lymphocyte and monocyte values were also significantly decreased while leukocyte count was significantly increased. The results obtained by micronucleus assay showed significantly a higher frequency of erythrocyte with micronuclei, blebbed nuclei, lobed, notched, heteropicnotic nuclei, binucleated and pear shape erythrocyte. The serum analysis showed that the concentration of different enzymes and lipid peroxidation products were significantly increased in exposed fish. It can be concluded that triazophos poses adverse hemato-biochemical and DNA damage effects in aquatic organisms.

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Section: ×1000 Giemsa Stainmentioning

confidence: 45%

Section: ×1000 Giemsa Stainmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hemato-biochemical and Genetic Damage Caused by Triazophos in Fresh Water Fish, Labeo rohita

Ghaffar¹,

Hussain²,

Khan³

et al. 2015

IJAB

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“…Different morphological changes in erythrocytes (Table 4) of fish exposed to arsenic and urea could have occurred due to increased generation of lipid peroxidation. Moreover, toxicants with oxidative stress potential might assault DNA leading to damage at molecular level (Jha, 2008;Hussain et al, 2014). Frequencies of nuclear and morphological anomalies in erythrocytes of fish such as irregular nucleus shape, poikilocytosis, binuclear, and micronuclei indicate genotoxic effects which could have taken place due to arsenic intoxication (Ergene et al, 2007;Strunjak-Perovic et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Ghaffar¹,

Hussain²,

Aslam³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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This study was conducted to assess hemato-biochemical and different protoplasmic changes, which are indicators of genotoxicity in erythrocytes of Labeo rohita exposed to arsenic and urea. For this purpose, 20 fish were kept in glass aquaria for acclimatization (5 days), randomly divided into five equal groups: A (control) and exposed to arsenic (in mg/L) + urea (in g/L) B (8+0.2), C (10+0.4), D (12+0.6) and E (15+0.8), respectively for 9 days. Blood samples with anticoagulant were collected at 3 rd , 6 th and 9 th experiment days for hematology. Serum was separated from blood samples collected without anticoagulants for biochemical studies. Results showed that exposure with arsenic and urea in higher concentrations (groups D and E) significantly decreased (P<0.05) erythrocyte counts, hemoglobin concentration, packed cell volume, mean corpuscular hemoglobin concentration, lymphocyte, and monocyte counts, accompanied by significant increase in mean corpuscular volume and leucocyte counts than those in control group (A). Alanine and aspartate amino transaminase activities at 6 th and 9 th days in high dose (groups D and E) were significantly increased whereas glucose and total protein decreased (P<0.05). It is concluded that arsenic has hemato-biochemical and genotoxic effects even at the low dose rate in the presence of nitrogenous chemicals in aquatic ecosystem.

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Ontogeny of the testicular excurrent duct system of male Japanese quail (Coturnix japonica): A histological, ultrastructural, and histometric study

Ibrahim

Zakariah

Molele

et al. 2021

Microscopy Res & Technique

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The testicular excurrent duct system undergoes several physiological and morphological changes during the reproductive stage or breeding season in mammals, birds, and reptiles. Studies on normal age-related histomorphological changes in the excurrent duct system of Japanese quails (Coturnix japonica) remain unreported, despite the extensive use of this bird as an avian model in research studies. The current study investigated the histological, ultrastructural, and histometric changes in the testicular excurrent duct system of the Japanese quail during three reproductive stages, namely prepubertal, pubertal, and adult. Simple squamous to low cuboidal cells formed the epithelia of the rete testis in prepubertal and pubertal birds, while in adult birds the lining was low cuboidal to cuboidal. In pubertal and adult birds, the nonciliated Type I epithelial cells of the proximal efferent duct displayed a subapical endocytotic apparatus comprising coated pits, coated apical tubules, and endosomes. There was a significant increase (p ≤ .001) in epithelial heights of all ducts of the excurrent duct system in the mature, sexually active, adult birds when compared to the other age groups. The luminal and tubular diameters, and the cross-sectional areas of efferent ducts and the epididymal duct unit increased significantly (p ≤ .001) with age. It is concluded that the morphology and morphometry of the excurrent ducts of the testis of the Japanese quail change as birds mature.

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Clinico-hematological and tissue changes induced by butachlor in male Japanese quail (Coturnix japonica)

Cited by 44 publications

References 23 publications

Hemato-biochemical and Genetic Damage Caused by Triazophos in Fresh Water Fish, Labeo rohita

Hemato-biochemical and Genetic Damage Caused by Triazophos in Fresh Water Fish, Labeo rohita

Untitled

Ontogeny of the testicular excurrent duct system of male Japanese quail (Coturnix japonica): A histological, ultrastructural, and histometric study

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