Clinico-biochemical and Electrocardiographic Changes in Cattle Naturally Infected with Lumpy Skin Disease

Helal, Mahmoud Atef Youssef; Marawan, Marawan A.; Bahgy, Halla E.K. El

doi:10.5455/ajvs.20434

Cited by 3 publications

(3 citation statements)

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“…It is caused by the LSD virus (LSDV), also known as Neethling virus, a double-stranded DNA virus of the Poxviridae family that spreads by blood-sucking insects. This condition is characterized by fever, lethargy, and the sudden appearance of skin nodules that cover the entire body, mucous membranes, and internal organs [2][3][4]. Lesions in internal organs can lead to respiratory and gastrointestinal problems, including dyspnea, pneumonia, and ruminal atony [5].…”

Section: Introductionmentioning

confidence: 99%

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Oxidative stress, biochemical, and histopathological changes associated with acute lumpy skin disease in cattle

et al. 2022

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Background and Aim: Lumpy skin disease (LSD) is a highly infectious endemic viral disease of cattle in Africa and the Middle East. The objectives of this study were to assess histopathological changes in cattle infected with LSD and measure serum malondialdehyde (MDA – oxidant) and total anti-oxidant capacity (TAC – anti-oxidant), trace elements (zinc, copper, and iron), cytokines (interleukin [IL]-1β, IL-6, and tumor necrosis factor-alpha [TNF-α]), haptoglobin (Hp), serum amyloid A (SAA), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), blood urea nitrogen (BUN), and creatinine concentrations. Materials and Methods: Blood samples were collected from a total of sixty native and mixed breed cattle; (healthy; n = 25) and (LSD diseased; n = 35). Serum concentrations of MDA and TAC were measured by colorimetric methods. Serum IL-1β, IL-6, IL-10, TNF-α, Hp, and SAA concentrations were determined using human-specific enzyme-linked immunoassay kits. Results: Serum MDA, cytokine (IL-1β, IL-6, and TNF-α), Hp, SAA, AST, ALT, CPK, BUN, and creatinine concentrations were significantly higher, while TAC, IL-10, zinc, copper, and iron concentrations were significantly lower in LSD compared to healthy cattle (p < 0.05). Cows and exotic mixed breed cattle were at higher risk of LSD oxidative stress than bulls and local breeds (p < 0.05). Age was not associated with the risk of LSD (p > 0.05). Histologically, there was extensive tissue necrosis, severe vasculitis, mononuclear cell infiltration, and intracytoplasmic inclusion bodies. Conclusion: LSD is associated with pro-oxidative and pro-inflammatory states from imbalances that favor pro-oxidant and pro-inflammatory factors in the detriment of anti-oxidant and anti-inflammatory factors, leading to organ dysfunction and ultimately death. Oxidative stress is more frequent in cows and mixed breed cattle than in bulls and local breeds. Keywords: acute phase proteins, cytokines, lumpy skin disease, malondialdehyde, total anti-oxidant capacity.

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Section: Introductionmentioning

confidence: 99%

“…Lesions in internal organs can lead to respiratory and gastrointestinal problems, including dyspnea, pneumonia, and ruminal atony [5]. Lumpy skin disease has economic implications due to weight loss, reduced milk production, abortions, infertility, damaged hides, and death [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress, biochemical, and histopathological changes associated with acute lumpy skin disease in cattle

et al. 2022

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“…MDA level in cattle with cutaneous papilloma was significantly higher than in healthy cattle [ 24 ]. In humans, high MDA levels have been detected in skin cancers such as basal cell carcinoma and non-melanoma skin carcinoma tissue that has been exposed to UV [ 39 ]. However, the available studies that describe blood antioxidant and lipid peroxidation status in skin diseases of cattle are few [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Regression of bovine cutaneous papillomas via ivermectin-induced immunostimulant and oxidative stress

Saied

2021

J Adv Vet Anim Res

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Objective: Ivermectin (IVM) could be used effectively to treat bovine cutaneous papillomatosis, a widespread viral skin disease that causes major economic losses in cattle. This study aimed to evaluate the regression of bovine cutaneous papillomas induced by IVM by estimating oxidative stress markers, besides clinicopathological and hematological findings. Materials and Methods: Twenty naturally infected animals with cutaneous papillomatosis were chosen randomly and diagnosed clinically and histopathologically. All the infected animals were divided into groups: Group I ( n = 10), which received no treatment and was considered the control group. In Group II ( n = 10), the animals were subcutaneously injected at 0.2 mg/kg of IVM 2 weeks apart during the 90-day experimental period. Papilloma regression was tracked clinically, papilloma biopsies were taken for histopathological analysis, and blood samples were taken for hematological and oxidative parameter testing. Results: From the 15th to 45th day after receiving IVM, papillomas began to fade. Necrotic areas, ulcerations, and lymphocytic infiltration were found in the histopathological studies, besides a decrease in papilloma epidermal proliferation. total erythrocytes count, packed cell volume, total leucocytes count, and lymphocytes values were increased significantly, and a large decrease in glutathione peroxidase and glutathione reduced was identified as signs of IVM-induced oxidative stress. Conclusion: IVM has oxidative and immunostimulatory properties, and it can be used against cutaneous papillomatosis.

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Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle

Yanni¹,

Elden

Kamoura

et al. 2021

Journal of Applied Veterinary Sciences

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This study aimed to diagnose Lumpy skin desease by isolation and identification of LSD from suspected naturally infected cattle and to study its effect on immuno-biochemical parameters, inflammatory and oxidative stress markers. The study was conducted on 25 cattle, 1-3 years age from two cities in the Sharkia governorate. Ten clinically healthy cattle represented the control group and fifteen suspected diseased cases by LSD represented the infected group. The clinical signs of suspected cases showed anorexia, skin nodules all over the body, fever above 40˚C, edema in forelimbs and dewlap, enlarged superficial lymph nodes, retarded milk production, and abortion in some cases. The virological examination of LSDV from the samples of skin nodules was done. Isolation from skin nodules was done on MDBK cell line and embryonated chiken egg through chorioallantoic membrane route followed by identification using fluorescence technique . Molecular studies including phylogenetic analysis for three positive isolates showed similarity to previously locally isolated strains in Egypt and close relation to strains of SPPV. Immunobiochemical results revealed a significant increase in aspartate aminotransferase , alanine aminotransferase , alkaline phosphatase , Malondialdehyde , Tumor necrosis factor , Interleukin4,immunoglobulin M and immunoglobulin G . Meanwhile, a significant decrease in total protein, albumin, globulin, Glutathione peroxidase, phagocytic assay, calcium, inorganic phosphorous, sodium, and potassium with a nonsignificant change in magnesium was seen when compared to control ones. It could be concluded that the infection with LSD in cattle induce severe effects on immuno-biochemical, antioxidant parameters, and inflammatory markers causing great economic loss ‫ـــــــــــــــــــــــــــــــــــــــــ‬

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Clinico-biochemical and Electrocardiographic Changes in Cattle Naturally Infected with Lumpy Skin Disease

Cited by 3 publications

References 28 publications

Oxidative stress, biochemical, and histopathological changes associated with acute lumpy skin disease in cattle

Oxidative stress, biochemical, and histopathological changes associated with acute lumpy skin disease in cattle

Regression of bovine cutaneous papillomas via ivermectin-induced immunostimulant and oxidative stress

Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle

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