Clinically Applicable Labeling of Mammalian and Stem Cells by Combining Superparamagnetic Iron Oxides and Transfection Agents

Frank, Joseph A.; Miller, Brad; Arbab, Ali S.; Zywicke, Holly A.; Jordan, Elaine; Lewis, Bobbi K.; Bryant, L. Henry; Bulte, Jeff W.M.

doi:10.1148/radiol.2281020638

Cited by 628 publications

(548 citation statements)

References 28 publications

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“…These studies typically involve implanting SPIO-loaded cells into living subjects and tracking their movement and localization at specific tissues. 43 Various strategies have been employed to efficiently load cultured cells with enough SPIO to ensure sufficient contrast for in vivo imaging. 6 Of particular interest is the conjugation of membrane translocating signals such as HIV-Tat and polyArginine peptides to SPIO, which greatly enhance SPIO uptake into many cell types.…”

Section: Cell Trackingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Section: Cell Trackingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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“…Moreover, the use of transfection media [146], polyamines, lipids, or dendrimers [147] can result in enhanced cellular SPIO incorporation. Due to the partially complicated labeling protocols, simplified strategies using clinically approved materials (e. g. Endorem ® /Feridex ® , Superfect ® , Lipofectamine Plus ® , poly-Llysine (PLL), protamine) and low cell toxicity have been described in recent years [148,149]. While most studies do not describe any significant changes in cell behavior (e. g. apoptosis rate, proliferation index, differentiation behavior) [150,151], individual studies show changes in migration behavior and the ability to form colonies [152], in cell vitality [153], in differentiation behavior [154], or in the expression pattern [152].…”

Section: Cell Labeling and Cell Imaging In Mrimentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Ittrich¹,

Peldschus²,

Raabe³

et al. 2013

Fortschr Röntgenstr

123

101

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Superparamagnetic iron oxide nanoparticles (SPIO) can be used to image physiological processes and anatomical, cellular and molecular changes in diseases. The clinical applications range from the imaging of tumors and metastases in the liver, spleen and bone marrow, the imaging of lymph nodes and the CNS, MRA and perfusion imaging to atherosclerotic plaque and thrombosis imaging. New experimental approaches in molecular imaging describe undirected SPIO trapping (passive targeting) in inflammation, tumors and associated macrophages as well as the directed accumulation of SPIO ligands (active targeting) in tumor endothelia and tumor cells, areas of apoptosis, infarction, inflammation and degeneration in cardiovascular and neurological diseases, in atherosclerotic plaques or thrombi. The labeling of stem or immune cells allows the visualization of cell therapies or transplant rejections. The coupling of SPIO to ligands, radio- and/or chemotherapeutics, embedding in carrier systems or activatable smart sensor probes and their externally controlled focusing (physical targeting) enable molecular tumor therapies or the imaging of metabolic and enzymatic processes. Monodisperse SPIO with defined physicochemical and pharmacodynamic properties may improve SPIO-based MRI in the future and as targeted probes in diagnostic magnetic resonance (DMR) using chip-based ?NMR may significantly expand the spectrum of in vitro analysis methods for biomarker, pathogens and tumor cells. Magnetic particle imaging (MPI) as a new imaging modality offers new applications for SPIO in cardiovascular, oncological, cellular and molecular diagnostics and therapy. Key Points: Citation Format:

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“…The cell labeling solution was prepared using 25 g of superparamagnetic iron oxide agent (Feridex, Berlex Laboratories) incubated for 60 min with 375 ng of poly-Llysine (Sigma) in Hanks' balanced salt solution and 2% ES cells qualified FBS (14,15). The mESC were removed from the cell culture plates by 0.05% Cellgro trypsin with EDTA (Mediatech) and then incubated with the magnetic labeling solution for 24 h.…”

Section: Labeling Of Mescmentioning

confidence: 99%

Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 t

Arai

Kofidis

Bulte

et al. 2005

Magnetic Resonance in Med

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106

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Clinically Applicable Labeling of Mammalian and Stem Cells by Combining Superparamagnetic Iron Oxides and Transfection Agents

Abstract: Magnetic labeling of mammalian cells with use of ferumoxides and TAs is possible and may enable cellular MR imaging and tracking in experimental and clinical settings.

Cited by 628 publications

References 28 publications

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 t

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