Clinical viability of Fungitell, a new (1→3)-β-d-glucan measurement kit, for diagnosis of invasive fungal infection, and comparison with other kits available in Japan

Yoshida, Koichiro; Shoji, Hisashi; Teratani, Takuma; Niki, Yoshihito

doi:10.1007/s10156-010-0198-6

Cited by 41 publications

(36 citation statements)

References 16 publications

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“…For example, the WAKO assay was less sensitive (0.63 and 0.75, respectively) than the Fungitell assay but more specific (0.97 and 0.79, respectively). However, we found that the Fungitec kit had the highest SEN value (0.86), which was not in line with the results reported by Yoshida et al (35). The reasons for the discrepancies could be partly related to the retrospective design that they used, which may render the results to be biased.…”

Section: Discussioncontrasting

confidence: 55%

“…The serum BG values measured with different kit showed some differences, possibly because various standard β-glucans, blood pretreatment methods, and different origins of lysates are used in each kit (29,30). Yoshida et al (35) reported that the kit with the highest SEN was Fungitell, followed by Fungitec, whereas the WAKO kit had an extremely low SEN value. Conversely, compared with the Fungitell and Fungitec kits, the WAKO kit had a higher SPE value.…”

Section: Discussionmentioning

confidence: 99%

“…The reasons for the discrepancies could be partly related to the retrospective design that they used, which may render the results to be biased. Because such differences among these kits could result in some clinical problems (35), further prospective cohort studies are necessary to clarify the clinical efficacy of these kits. Since these variations did not account fully for the heterogeneity, searching other differences was very important in our meta-analysis.…”

Section: Discussionmentioning

confidence: 99%

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Diagnosis of Invasive Fungal Disease Using Serum (1.RAR.3)-.BETA.-D-Glucan: A Bivariate Meta-Analysis

et al. 2011

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Background The (1 3)-β-D-Glucan (BG) assay has been approved for diagnosing invasive fungal disease (IFD). However, the test performance has been variable. We conducted a meta-analysis to determine the overall accuracy of BG assay for diagnosing IFD. Methods The sensitivity, specificity, and positive and negative likelihood ratios (PLR and NLR, respectively) of BG for diagnosing IFD were pooled using a bivariate meta-analysis. We also performed subgroup analyses. Results Twelve reports, including 15 studies, were included for the analysis (proven and probable IFD vs possible or no IFD). The sensitivity, specificity, PLR and NLR were 0.76 (95% CI, 0.67-0.83), 0.85 (95% CI, 0.73-0.92), 5.05 (95% CI, 2.71-9.43), and 0.28 (95% CI, 0.20-0.39), respectively. Subgroup analyses showed that the BG assay had higher specificities for patients with hematological disorders and a positive BG result with two consecutive samples. The combination of galactomannan and BG increased the specificity value to 0.98 (95% CI, 0.95-0.99) for diagnosing invasive aspergillosis. Conclusion Serum BG determination is clinically useful for diagnosing IFD in at-risk patients, especially for hematology patients. The combination of galactomannan and BG was sufficient for diagnosing invasive aspergillosis. Since the BG assay is not absolutely sensitive and specific for IFD, the BG results should be interpreted in parallel with clinical findings.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diagnosis of Invasive Fungal Disease Using Serum (1.RAR.3)-.BETA.-D-Glucan: A Bivariate Meta-Analysis

et al. 2011

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“…According to the predefined cutoff value of 11 pg/mL by Wako assay, the sensitivity, specificity, PPV and NPV were 90%, 83.3%, 94.7% and 71.4%, respectively. These results are comparable to the ones published by Yoshida et al (17), and look reliable. Of course Karageorgopoulos et al in his Meta-analysis study reported a total sensitivity of 77% and specificity of 85% that shows a lower sensitivity than specificity for BDG assay (19).…”

Section: Discussionsupporting

confidence: 92%

“…The commercial kits used in these countries have different measurement methods (colorimetric or turbidimetric) and cutoff values thus sensitivity, specificity, PPV and NPV reported for each kit and for each experiment have shown large differences; then judgment on positive and negative results could be a laborious work leading to several clinical problems (17). Since a lot of factors could interfere with the results, it is very important that each laboratory sets up the test and calculates its own cutoff values and other diagnostic efficiency criteria.…”

Section: Discussionmentioning

confidence: 99%

β-D-Glucan Assay in Diagnosis and Monitoring the Systemic Candidiasis in a Rat Model

Khodadadi

Mirhendi

Makimura

et al. 2014

Jundishapur J Microbiol

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Background:Determination of β-D-Glucan (BDG) in the serum aids to diagnose the invasive fungal infections. The current study evaluated the diagnostic potential value of BDG assay in monitoring the disease in experimental systemic candidiasis in a rat model. The results can provide a useful preliminary data to improve this approach in developing countries.Objectives:The present study aimed to evaluate β-D-Glucan assay in diagnosis and monitoring the systemic candidiasis in a rat model.Materials and Methods:Twenty one rats were infected with 106 Candida albicans blastospore per rat. Twelve rats were considered as the negative controls (six immunocompromised rats without infection and six intact rats). During a week, every 24 hours the BDG sera level was determined by both Fungitell and Wako kits. To confirm the systemic infection in each rat, the suspensions of their internal organs were cultivated on agar plates and the number of colony forming units (CFU) of C. albicans was counted.Results:All the infected rats were positive with BDG tests. An increasing level of BDG was observed during early days after injection. The cutoff value for discrimination of BDG positive sera was obtained from the negative sera by the Fungitell kit. The sensitivity, specificity, positive and negative predictive values assessed for the Fungitell kit were 95%, 66.6%, 90.47% and 80%, respectively. These criteria for those of Wako were 90%, 83.3%, 94.7% and 71.4%, respectively.Conclusions:While BDG assay seems to be a sensitive and specific adjunctive tool to diagnose and monitor the experimental systemic candidiasis, it seems that measuring the positive cutoff value in different laboratory conditions is necessary for favorable establishment of these tests.

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Comparison of two β-D-glucan assays for detecting invasive fungal diseases in immunocompromised patients

Song¹,

Kim²,

Park³

et al. 2021

Diagnostic Microbiology and Infectious Disease

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Clinical viability of Fungitell, a new (1→3)-β-d-glucan measurement kit, for diagnosis of invasive fungal infection, and comparison with other kits available in Japan

Cited by 41 publications

References 16 publications

Diagnosis of Invasive Fungal Disease Using Serum (1.RAR.3)-.BETA.-D-Glucan: A Bivariate Meta-Analysis

Diagnosis of Invasive Fungal Disease Using Serum (1.RAR.3)-.BETA.-D-Glucan: A Bivariate Meta-Analysis

β-D-Glucan Assay in Diagnosis and Monitoring the Systemic Candidiasis in a Rat Model

Comparison of two β-D-glucan assays for detecting invasive fungal diseases in immunocompromised patients

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