Clinical value of changes of the transmigration activity of leukocytes in the diagnosis of severe preeclampsia

Abstract

Aim. To identify additional criteria for the diagnosis of severe preeclampsia (PE) in pregnant women based on an investigation of the expression of the early adhesion molecules, chemokine receptors, and markers of endothelial dysfunction in venous blood neutrophils and monocytes. Material and methods. One hundred and fifty patients with PE (74 with moderate PE, 76 with severe PE) and 70 women with a physiological course of pregnancy were tested for venous blood neutrophils and monocytes expressing adhesion molecules CD62L, CD11b, chemokine receptors CCR2 and CX3CR1, and the number of circulating endothelial cells (CECs). Results. When compared with women having uneventful pregnancy and patients with moderate PE, patients with severe PE had significantly lower levels of CD62L+neutrophils, but higher levels of CX3CR1+monocytes and neutrophils, and a greater number of CECs. The diagnostic thresholds for these parameters are informative in the diagnosis of PE severity. Conclusion. Measuring the relative levels of CD62L+, CX3CR1+ neutrophils and the number of CECs in the blood can be used for stratifying severity of PE with greater accuracy.

Pathophysiological impact of CXC and CX3CL1 chemokines in preeclampsia and gestational diabetes mellitus

“…According to Kudryashova et al (2019) , there is a correlation between low levels of CD62L+, CD99 + , CXCR1+ neutrophils, serum CXCL8, and adversely affected blood vessel elastic properties. In addition, another study found that CX3CR1+ monocyte and neutrophil levels were higher in PE patients ( Panova et al, 2018 ). These findings clarified that, possibly, in PE, activated endothelial cells themselves regulate how tightly neutrophils adhere to the endothelium.…”