Clinical utility of lipoarabinomannan antibody in pleural fluid for the diagnosis of tuberculous pleurisy

Yokoyama, Toshinobu; Rikimaru, Toru; Kinoshita, Takashi; Kamimura, Tomoko; Oshita, Yuichi; Aizawa, Hisamichi

doi:10.1007/s10156-004-0366-7

Cited by 9 publications

(7 citation statements)

References 5 publications

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“…Anti-P32 levels were reported to be higher among five patients with pleural TB, but the sensitivity and specificity were not reported [43]. Sensitivity to anti-lipoarabinomannan (LAM) and to anti-TB glycolipid antibodies was very low in other studies, although specificity was high [44][45][46][47].…”

Section: B-cell Response (Antibody Detection)mentioning

confidence: 99%

Novel tests for diagnosing tuberculous pleural effusion: what works and what does not?

Trajman

Pai²,

Dheda³

et al. 2008

European Respiratory Journal

140

121

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Tuberculous pleuritis is a common manifestation of extrapulmonary tuberculosis and is the most common cause of pleural effusion in many countries. Conventional diagnostic tests, such as microscopic examination of the pleural fluid, biochemical tests, culture of pleural fluid, sputum or pleural tissue, and histopathological examination of pleural tissue, have known limitations. Due to these limitations, newer and more rapid diagnostic tests have been evaluated. In this review, the authors provide an overview of the performance of new diagnostic tests, including markers of specific and nonspecific immune response, nucleic acid amplification and detection, and predictive models based on combinations of markers. Directions for future development and evaluation of novel assays and biomarkers for pleural tuberculosis are also suggested.

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Section: B-cell Response (Antibody Detection)mentioning

confidence: 99%

Novel tests for diagnosing tuberculous pleural effusion: what works and what does not?

Trajman

Pai²,

Dheda³

et al. 2008

European Respiratory Journal

140

121

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“…На предметные стекла наносили по 25 мкл полученной клеточной суспензии и инкубировали в течение 40 мин при комнатной температуре во влажной камере. Осевшие клетки фиксировали в течение 8-10 мин в холодном ацетоне, после чего наносили на стекла по 15 чего отмывали раствором Хэнкса из расчета 1 мл раствора на одну пробу. Подсчет флюоресцирующих клеток проводили на люминесцентном микроскопе («Micros», Австрия) предварительно добавляя 15 мкл глицероля.…”

Section: материалы и методыunclassified

Lymphocyte Subpopulations of Blood and Pleural Exudate in MBT-Negative and MBT-Positive Tuberculous Pleurisy

Юрьева¹,

Воронкова²,

Уразова³

et al. 2014

Med.Imm.Rus

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Резюме. Были изучены показатели субпопуляционного состава лимфоцитов периферической крови и плеврального экссудата у больных с различными вариантами туберкулезного плеврита. Было установлено, что у пациентов с МБТ-негативным и МБТ-позитивным вариантами туберкулезного плеврита наблюдается снижение количества CD3-, CD4-позитивных лимфоцитов и, напротив, повышение числа CD8 + и CD16 + лимфоцитов более выраженное у лиц с МБТ-негативным вариантом плеврита. В плевральной жидкости у больных туберкулезными плевритами число CD3 + лимфоцитов оказалось значимо более высоким относительно соответствующих параметров в периферической крови. Авторами высказывается предположение, что лимфоцитоз в плевральной жидкости-это результат миграции клеток из периферической крови в плевральную полость. Установлено, что при экссудативном плеврите туберкулезного генеза миграции в плевральную полость подвержены Т-клетки с фенотипом CD4 + и CD8 + , а при МБТ-позитивном варианте плеврита еще и CD16 + .

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“…In the earlier published studies, several non-bacteriological diagnostic parameters have been evaluated for the early laboratory diagnosis of TPE. These include (a) histopathology in pleural biopsy (Diacon et al, 2002), (b) nucleic acid amplification assay (Dinnes et al, 2007;Trajman et al, 2007), (c) estimation of biomarkers such as adenosine deaminase (Chen et al, 2004;Trajman et al, 2007), (d) antibody to mycobacterial glycolipids (Morimoto et al, 2006), (e) lipoarabinomannan (Yokoyama et al, 2005) and (f) IFN-γ (Greco et al, 2003;Jiang et al, 2007). These above diagnostic approaches do hold promise, but the sensitivities and specificities reported in these studies are highly variable and hence restricted the routine application as a diagnostic tool in patients with TPE.…”

Section: Introductionmentioning

confidence: 99%

Estimation of cord factor antigen of Mycobacterium tuberculosis in pleural fluid and its diagnostic significance

Rose¹

2012

J. Infect. Dis. Immun.

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In this study, a sandwich enzyme linked immunosorbent assay (ELISA) was developed to measure the concentration of cord factor (CF) antigen of M. tuberculosis in the pleural fluids of 42 patients with tuberculous pleural effusion (TPE). The assay was also simultaneously performed in 43 pleural fluid specimens with malignant pleural effusion (MPE). The assay detected CF antigen in 83.3% in patients with TPE effusion in whom M. tuberculosis was not demonstrated in pleural fluid specimens by bacteriological methods. The data also indicated that cord factor antigen was not present in significant titres in any one of the 43 pleural fluids of patients with MPE. It is concluded that estimation of cord factor of M. tuberculosis in pleural fluid specimens by the sandwich ELISA as developed in this study will support the diagnosis of tuberculous aetiology in pleural fluids, particularly in those patients in whom M. tuberculosis was not demonstrated by bacteriological methods.

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Clinical utility of lipoarabinomannan antibody in pleural fluid for the diagnosis of tuberculous pleurisy

Cited by 9 publications

References 5 publications

Novel tests for diagnosing tuberculous pleural effusion: what works and what does not?

Novel tests for diagnosing tuberculous pleural effusion: what works and what does not?

Lymphocyte Subpopulations of Blood and Pleural Exudate in MBT-Negative and MBT-Positive Tuberculous Pleurisy

Estimation of cord factor antigen of Mycobacterium tuberculosis in pleural fluid and its diagnostic significance

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