Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong

Evaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia

“…RNA extracts of a few samples were also applied successfully to monitor drug resistance mutations in pol gene sequence as described previously (data not shown) (Yam et al, 2006). The sensitivity of NucliSens EasyQ TM assay in this study, reported as the detection limit and the 95% positive rate, is comparable to the reported value of COBAS Amplicor TM (Sun et al, 1998).…”

“…For subtype determination using HIV pol gene analysis, the same RNA extract from NucliSens MiniMAG TM extractor was amplified by RT-PCR followed by direct sequencing of the PCR amplicons (Yam et al, 2006). The Pregap4 and Gap4 modules of Staden Package (Medical Research Council-Laboratory of Molecular Biology, England) were used for sequence assembly and the HIV-1 subtype was determined using Stanford HIVdb database (http://hivdb6.stanford.edu).…”

High prevalence of primary Enfuvirtide (ENF) resistance-associated mutations in HIV-1-infected patients in Hong Kong

“…7,8 The use of pol sequences for HIV-1 genotyping was supported by other reports. 9,10 The majority of samples belonged to subtypes B (100/187; 53.4%) and CRF01 AE (53/187; 28.1%).…”

Evaluation of an in-house genotyping resistance test for HIV-1 drug resistance interpretation and genotyping

“…HIV-1 non-B viruses, especially CRF01 AE, are predominant in Hong Kong and other South-East Asian developing countries (Ariyoshi et al, 2003;Tovanabutra et al, 2004;Laeyendecker et al, 2005;Yam et al, 2006). Recognizing the growing demand for a cost-effective GRT on patients with HAART in our region, this study evaluated a novel HIV-1 inhouse GRT in terms of its ability of drug resistance-related mutation detection in different HIV-1 subtypes by comparing with results of the commercial ViroSeq TM genotyping system.…”

“…Other than HIV-1 subtype B, the circulating recombinant form AE (CRF01 AE), and subtype C is the prevalent strain circulating in Hong Kong and other Asian countries (Ariyoshi et al, 2003;McCutchan, 2006;Yam et al, 2006). Recent studies showed that the ViroSeq TM and TruGene TM HIV-1 Genotyping Systems (Eshleman et al, 2004;Jagodzinski et al, 2003) are applicable to various HIV-1 subtypes; however, problems with non-B strains have occasionally been reported (Beddows et al, 2003;Fontaine et al, 2001;Mracna et al, 2001).…”