Clinical utility of cycle threshold values in the context of COVID-19

Rao, Sonia N.; Manissero, Davide; Steele, Victoria; Pareja, Josep

doi:10.21203/rs.3.rs-41867/v1

Cited by 12 publications

(5 citation statements)

References 32 publications

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“…The cycle threshold (Ct) values of RT-PCR represent the number of amplification cycles that are needed by the target gene to surpass a certain threshold level. Ct values are inversely proportional to viral load and can be used as an indirect way of quantifying viral RNA copy numbers [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Correlation of Viral Load With the Clinical and Biochemical Profiles of COVID-19 Patients

Atique¹,

Ghafoor

Javed³

et al. 2021

Cureus

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Background/objectiveCoronavirus infectious disease (COVID-19) is a novel disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-COV-2). Some studies have shown that disease severity according to clinical and biochemical parameters are in direct relation to viral load while others have found no direct correlation. In this study, the COVID-19 cycle threshold (Ct) value, which is taken as a direct indicator of the viral load, has been correlated with the biochemical and clinical parameters in COVID-19 patients. MethodsIn this cross-sectional, retrospective, and single-center study, 365 patients admitted with COVID 19 were divided into three groups according to their Ct values obtained from reverse transcription-polymerase chain reaction RT-PCR as 1 (9-20), 2 (21-30), and 3 (31-40). The correlation of the COVID-19 Ct value with biochemical parameters and clinical presentation (taken as mild, moderate, and severe) was done and analyzed. The chi-square test was used for the correlation and calculated by using SPSS V-24.0 (IBM Corp., Armonk, NY). p-value <0.05 was considered significant statistically. ResultsDisease severity levels (mild, moderate, and severe) correlated in group 1 (Ct value 9 to 20), 2 (Ct value 21 to 30), and 3 (Ct value 31 to 40) but no significance was found between disease severity levels and the Ct value groups' p-value (>0.05). All the biochemical parameters analyzed (alanine transaminase (ALT), aspartate aminotransferase (AST), albumin, bilirubin, c-reactive protein (CRP), lactate dehydrogenase (LDH), ferritin, D-dimer, and total leucocyte count (TLC)) showed a significant p-value (<0.05) in all the three groups studied. Procalcitonin (PCT), however, did not show any significant value in any of the groups studied.In the intergroup assessment, it was found that the values of ALT, AST, albumin, CRP, ferritin, bilirubin, and TLC are maximum in group 2 with a downward trend in groups 1 and 2. Neutrophils and lymphocytes did not show any variations. LDH did not follow the trend of increasing viral load. ConclusionsThe severity of the disease was not statistically significant in the Ct value groups (p> 0.05). However biochemical parameters, i.e. ALT, AST, ALP, CRP, and bilirubin were statistically significant (p<0.05).Patients with COVID-19 should be closely monitored for the assessment of disease progression according to the above-mentioned biochemical parameters.

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Section: Introductionmentioning

confidence: 99%

Correlation of Viral Load With the Clinical and Biochemical Profiles of COVID-19 Patients

Atique¹,

Ghafoor

Javed³

et al. 2021

Cureus

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“…The disease COVID-19, caused by infection with SARS-CoV-2, is associated with a series of physiopathological mechanisms that mobilize a wide variety of biomolecules, especially immunological ones (Costela-Ruiz et al, 2020;Li et al, 2020). The gold standard for infection diagnosis is real-time reverse transcriptase PCR (RT-PCR) (Kevadiya et al, 2021;Rao et al, 2020;Younes et al, 2020). Following the emergence of the COVID-19 pandemic, D-dimer was established as a possible predictor of prognosis for COVID-19 patients.…”

Section: Introductionmentioning

confidence: 99%

Difference of Cycle Threshold Value, Oxygen Saturation and D-dimer to COVID-19 Vaccination

Redhono Harioputro,

Dasa Ramadhan,

Nurhayatun

et al. 2023

j. info kesehat.

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Many factors can affect the prognosis of COVID-19 patients, and Cycle Threshold value can be used to estimate the amount of virus in the body. Oxygen saturation and D-dimer are important components in determining the severity. This study aims to analyze the difference of CT value, oxygen saturation, D-dimer, and degree of severity on vaccination state. This study is the observational analytic study of patients admitted to the isolation ward of a referral hospital in Surakarta, Indonesia. The analysis covered assessing the normality of the data, the Kruskal-Wallis correlation test, and ANOVA to assess the differences between variables. A total of 154 patients with, 65.6% of them did not participate in the full vaccination program. The results of the analysis showed a significant difference between the D-dimer and the degree of severity with a p-value of < 0.05, while the CT value and oxygen saturation had no significant difference with the vaccination state. Vaccination showed a significant difference with D-dimer and the degree of severity of COVID-19 infection, so giving a complete vaccine can reduce the severity of COVID-19 patients, and we recommended to be able to complete the vaccine for the whole community.

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“…3 Cycle threshold values of real-time polymerase chain reaction (PCR), which is the gold-standard diagnostic method for COVID-19, are representative of assumed viral loads in the tested specimen. 4 The role of viral loads in COVID-19 severity, mortality, and transmission is still a matter of question.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Alpha Variant (B.1.1.7), Viral Load, and Age on the Clinical Course of Pediatric COVID-19 Patients

Unalan-Altintop

Celep

Milletli-Sezgin

et al. 2022

J Pediatr Infect Dis

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Objective The research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mainly consists of adult patients, leaving its impact on children understudied. This study aims to investigate the correlations between viral load, clinical course, age, and Alpha variant (B.1.1.7) in children. Methods The study was conducted on children under the age of 18 years, who were admitted to Amasya University Sabuncuoglu Serefeddin Research and Training Hospital in Turkey between February and April 2021. ΔCt values, which were obtained by real-time polymerase chain reaction (PCR), were analyzed to estimate the viral loads of the patients. Alpha variant (B.1.1.7) positivity was determined by real-time PCR. Results There was no difference between estimated viral loads of different clinical courses (p > 0.05), or between asymptomatic and symptomatic patients (p > 0.05). Viral loads were found to decrease with increasing age (p = 0.002). Also, a higher rate of symptomatic disease was found in children under the age of 4 years (p < 0.05). Alpha variant (B.1.1.7) was not found to be associated with severe disease in children (p > 0.05). Conclusion Our results demonstrate higher viral loads and symptomatic disease in children under the age of 4 years. Alpha variant (B.1.1.7) was not found to be related to disease severity. There has not been a consensus on the vaccination of the pediatric population worldwide. More studies are needed to understand the viral kinetics of SARS-CoV-2 and its severity on children to build effective vaccination strategies in children as public health restrictions are eased.

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Clinical utility of cycle threshold values in the context of COVID-19

Cited by 12 publications

References 32 publications

Correlation of Viral Load With the Clinical and Biochemical Profiles of COVID-19 Patients

Correlation of Viral Load With the Clinical and Biochemical Profiles of COVID-19 Patients

Difference of Cycle Threshold Value, Oxygen Saturation and D-dimer to COVID-19 Vaccination

The Impact of Alpha Variant (B.1.1.7), Viral Load, and Age on the Clinical Course of Pediatric COVID-19 Patients

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