Clinical utility of a passive immune basophil activation test for the analysis of allergic transfusion reactions

Abstract

The passive immune basophil activation test may help clarify the causal relationship between allergic transfusion reactions and transfused blood, even when patients experience myelosuppression.

Immunoglobulin (Ig)G antibodies against IgE identified by basophil activation test as the putative causative agent of a serious allergic transfusion reaction: potential utility of the test as a new safety measure for allergic transfusion reactions

“…Replacement of IgE bound on basophils was performed with the two‐step procedure that has previously been reported . The plasma used for replacement of receptor‐bound IgE was derived from 11 representative healthy laboratory personnel, who showed different reactivity of basophils for the FFP‐ATR1.…”

“…Evaluation of the binding capacity of IgG autoantibody against human IgE using basophils whose surface IgE, bound on FcεR, was replaced with IgE originated from other healthy subjects Replacement of IgE bound on basophils was performed with the two-step procedure that has previously been reported. 31 The plasma used for replacement of receptorbound IgE was derived from 11 representative healthy laboratory personnel, who showed different reactivity of basophils for the FFP-ATR1. We defined basophils in the cell pellet, whose own receptor-bound IgE was replaced by third-party IgE, as "quasi-basophils.…”

“…To explore the possibility above, we utilized the passive immune BAT system. 31 We prepared two groups of quasi-basophils; they have the same source of basophils, but their replaced IgE was different. Each type of quasi-basophils possessed IgE derived from either the high-or the low-responder subjects.…”

“…These markers allow the identification of activated basophils by flow cytometry (FCM). In the past decade, the BAT was also applied to transfusion medicine . In previous studies, we demonstrated that the BAT, performed using patient blood and the supernatants (SNs) of transfused blood components, was able to elucidate not only the causative relationship between ATRs and the transfusion but also the mechanisms behind ATRs …”

“…In the past decade, the BAT was also applied to transfusion medicine. 2,9,10,13,[26][27][28][29][30][31] In previous studies, we demonstrated that the BAT, performed using patient blood and the supernatants (SNs) of transfused blood components, was able to elucidate not only the causative relationship between ATRs and the transfusion but also the mechanisms behind ATRs. 25 The Japanese Red Cross Society performs a hemovigilance, which is similar to the "SHOT" in the United Kingdom, focusing on the relatively serious adverse effects.…”