Clinical usefulness of sperm DNA fragmentation testing

Influence of ejaculatory abstinence period on semen quality of 5165 normozoospermic and oligozoospermic Nigerian men: A retrospective study

“…29,30 ROS and RNS attack the mitochondrial and nuclear DNA causing increased sperm DNA fragmentation and apoptosis. [31][32][33] The observed reduction in sperm count with increasing ejaculatory abstinence duration in oligozoospermic semen may be, at least partly, due to increased ROS-and RNS-induced sperm damage.…”

“…During transit and storage, spermatozoa are exposed to a higher level of ROS and reactive nitrogen species (RNS) than elsewhere in the genital tract 29,30 . ROS and RNS attack the mitochondrial and nuclear DNA causing increased sperm DNA fragmentation and apoptosis 31–33 . The observed reduction in sperm count with increasing ejaculatory abstinence duration in oligozoospermic semen may be, at least partly, due to increased ROS‐ and RNS‐induced sperm damage.…”

Microbiological Diagnosis of Male Fertility beyond Semen Analysis

“…The absent of DNA repair mechanism in spermatid and spermatozoa is a major drawback in the life of a sperm cell because they are susceptible to both internal and external environmental factors that can cause damage (Alex et al, 2017). There are many types of damages or abnormalities that can occur to a sperm DNA structure depending on the rate of individual exposure to these internal and external factors such as antioxidant and ascorbic acid which can result to chromosomal rearrangement leading to chromosomal breaks and fragments compromising quality of spermatozoa and inefficiency in pregnancy sustainability (Jeremias and Witkin1996).…”

The role of female factors in the management of sperm DNA fragmentation

“…Dr. Varghese et al provided a comprehensive summary of their viewpoint on sperm DNA fragmentation (SDF) ( 1 ) in response to the practice recommendations by Dr. Agarwal et al ( 2 ). The authors raised the essential question by asking “whether SDF analysis adds useful information which can change the diagnosis or provide a better understanding of the prognosis”.…”

“…The authors raised the essential question by asking “whether SDF analysis adds useful information which can change the diagnosis or provide a better understanding of the prognosis”. Although Dr. Varghese et al considered the predictive value of SDF testing on sperm quality and outcomes of assisted reproductive technology (ART) as still controversial in general, the potential use of SDF in certain circumstances including unexplained infertility was recognized ( 1 ). Agarwal and colleagues reached a different conclusion on this issue from another perspective by ascertaining the clinical value of SDF evaluation by summarizing the literature which is more or less the same as those reviewed by Varghese et al ( 3 ).…”