Clinical usefulness of chemosensitivity testing using the MTT assay

Abstract

The results of in vitro chemosensitivity testing using the MTT assay of tumor cells from 140 patients were analyzed with reference to the clinical antitumor effects of the chemotherapy. One hundred and twenty-four (88.6%) of 140 specimens were successfully tested by the method of Mosmann (J Immunol Methods 65:55-63, 1983) with some modifications. When the results of the assay were compared with the clinical effects of chemotherapy in 22 patients with remaining measurable tumor lesions, the overall prediction rate was 86.4% (19/22). Among 31 patients with stage III-V gastric and colorectal carcinomas without remaining measurable tumor lesions, the survival rate of nine patients treated with drugs shown to be effective in the assay was significantly (P less than 0.05) better than that of 22 patients treated with drugs shown to be ineffective.

UCN‐01 (7‐Hydroxystaurosporine) Enhances 5‐Fluorouracil Cytotoxicity through Down‐regulation of Thymidylate Synthetase Messenger RNA

“…Evaluation of cytotoxicity We evaluated the in vitro chemosensitivity of tumor cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, Sigma, St. Louis, MO) assay reported by Mosmann 23) with some modifications as reported previously. [24][25][26] Cell suspensions were centrifuged, and tumor cells were suspended in DMEM supplemented with 10% fetal bovine serum (FBS) (JRH Bioscience, Lenexa, KS), diluted to 2×10 5 cells/ml, and plated into 96-well microplates (GIBCO) in a volume of 100 µl, resulting in 10 4 cells/well. Drug solutions were dissolved in DMEM and 100 µl was added to each well.…”

Histoculture drug response assay predicts the postoperative prognosis of patients with esophageal cancer

“…The reason for this high evaluability is that HDRA with MTT endpoint, as established and modified by Hoffmann et al (34) and Furukawa et al (24,35), enables a native-state histoculture including tissue architecture, tumor-stromal interaction and differentiated functions, a long-term cell culture, and long-term exposure to time-dependent anticancer drugs such as 5-Fu. However, a few studies have mentioned that over-estimation of drug efficacy, specifically false positive, was observed in using MTT endpoint assay for chemosensitivity test (36,37). These findings were observed mainly in the conventional monolayer cell cultures using MTT assay, not in HDRA with MTT endpoint.…”

Establishment of combined immuno-chemotherapy with systemically administered gemcitabine and intra-portal administration of interleukin-2 in murine models of liver metastases of pancreatic cancer

“…The sensitivity of GEM as an anticancer substance was evaluated at 72 h by MTT assay. The MTT assay conformed to the modification of the method (19)(20)(21) reported by Mosmann (22) i.e., the 3-(4,5-dimethylthiazol-2 yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) method. A suspension of 5x10 4 cells per ml of standard medium was prepared, and 200 μl of the suspension per well (10 4 cells/well) were dispersed on a 96-well microplate.…”