Clinical Trials of Immunotherapy of Cancer Utilizing Cytotoxic Cells

Ettinghausen, Stephen E.; Rosenberg, Steven A.

doi:10.1007/978-1-4684-6814-4_41

Cited by 1 publication

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“…This allows the TCRs from any cloned T cell to be cloned and incorporated into a gene vector. The suitability of T lymphocytes as a target for retroviral transduction has been demonstrated in a number of human gene therapy protocols such as transduction with the adenosine deaminase (ADA) gene in patients with ADA deficiency and transduction of tumour infiltrating lymphocytes with IL‐1β, IL‐12 or the neo marker [19–21]. The efficient transfer of TCRs from melanoma, human immunodeficiency virus and EBV‐specific T cells has demonstrated the general applicability of TCR transfer [10, 17, 22].…”

Section: Discussionmentioning

confidence: 99%

Retroviral Transfer of T‐Cell Receptor Genes Produces Cells with a Broad Range of Lytic Activity

2003

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The ability to transfer the T-cell receptor (TCR) for antigen using a retroviral vector has opened the door to a new paradigm for T-cell-based immunotherapy. Using recombinant TCRs, a population of activated T cells can now be redirected to recognize and lyse cellular targets according to the specificity afforded by the transduced TCR genes. To examine the range of lytic activity displayed by the transduced TCRs, transduced T cells were re-cloned by limiting dilution and quantitatively analysed for lytic activity. The lytic activity of the transduced TCRs varied considerably, as determined by the K m and V max of lysis. The lytic activity seen in the secondary clones generated from vector-transduced peripheral blood mononuclear cell demonstrated that one of the clones approached the lytic activity of the parental 'TCR donor' cytotoxic T-cell lymphocyte (CTL) clone, whereas the remainder demonstrated either reduced V max or reduced V max and K m . Thus, the lytic activity of a transduced TCR depends not only on its genetic sequence but also on the cellular context within which it is expressed. Analysis of TCR Vb transcript levels by real time polymerase chain reaction revealed that while total Vb gene expression was fairly constant, expression of the retrovirally transduced Vb chain varied greatly in transduced CD8 þ CTL clones.

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Section: Discussionmentioning

confidence: 99%