Clinical Treatment of Radiotherapy Tissue Damage by Lipoaspirate Transplant: A Healing Process Mediated by Adipose-Derived Adult Stem Cells

Rigotti, Gino; Marchi, Alessandra; Galiè, Mirco; Baroni, Guido; Benati, Donatella; Krampera, Mauro; Pasini, Anna Fratta; Sbarbati, Andrea

doi:10.1097/01.prs.0000256047.47909.71

Cited by 1,016 publications

(680 citation statements)

References 23 publications

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“…For more details see Supplementary Materials. (Figure 6b) were isolated from inguinal adipose tissues of three different FVB mice using standard protocols (Zuk et al, 2002;Rigotti et al, 2007). Briefly, SVF was obtained after collagenase digestion and seeded in culture.…”

Section: Methodsmentioning

confidence: 99%

“…After 1-2 weeks of culture a homogeneous cell population was obtained. These cells were identified as MSCs on the basis of their immunophenotype profile and their multilineage plasticity (Supplementary Data), as previously described Rigotti et al, 2007). Specifically, the positivity of CD106 (VCAM-1), CD73, CD29, CD44, CD90 and the lack of hematopoietic (anti-CD45, -CD14, -CD11c, CD123 and -CD34 monoclonal antibodies) and endothelial cell markers (with anti-CD31 monoclonal antibody) were assessed by means of cytofluorimetric analysis.…”

Section: Methodsmentioning

confidence: 99%

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Mesenchymal stem cells share molecular signature with mesenchymal tumor cells and favor early tumor growth in syngeneic mice

et al. 2007

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Tumor microenvironment in carcinomas recruits mesenchymal cells with an abnormal proangiogenic and invasive phenotype. It is not clear whether mesenchymal tumor cells (MTCs) derive from the activation of mature fibroblasts or from their stem cell precursors. However, stromal cell activation in tumors resembles in several aspects the mesenchymal rearrangement which normally occurs during reparative processes such as wound healing. Mesenchymal stem cells (MSCs) play a crucial role in developmental and reparative processes and have extraordinary proangiogenic potential, on the basis of which they are thought to show great promise for the treatment of ischemic disorders. Here, we show that MTCs have proangiogenic potential and that they share the transcriptional expression of the best-known proangiogenic factors with MSCs. We also found that MTCs and MSCs have the same molecular signature for stemness-related genes, and that when co-implanted with cancer cells in syngeneic animals MSCs determine early tumor appearance, probably by favoring the angiogenic switch. Our data (1) reveal crucial aspects of the proangiogenic phenotype of MTCs, (2) strongly suggest their stem origin and (3) signal the risk of therapeutic use of MSCs in tumorpromoting conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Mesenchymal stem cells share molecular signature with mesenchymal tumor cells and favor early tumor growth in syngeneic mice

et al. 2007

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“…In the case of breast reconstruction, autologous SVF cells are recombined with the patient's own lipoaspirate tissue for purposes of fat grafting. Similarly, fat grafting and adipose-derived stem cells can be used to ameliorate skin fibrotic changes following breast or head and neck tumor radiation therapy [48,49]. Thus, soft tissue defects are a logical therapeutic target for SVF cells and ASCs because they are returned to their tissue of origin.…”

Section: Clinical Studies-are We There Yet? Clinical Opportunitiesmentioning

confidence: 99%

Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Stem Cells: Let's Not Get Lost in Translation

et al. 2011

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Subcutaneous fat has emerged as an alternative tissue source for stromal/stem cells in regenerative medicine. Over the past decade, international research efforts have established a wealth of basic science and preclinical evidence regarding the differentiation potential and regenerative properties of both freshly processed, heterogeneous stromal vascular fraction cells and culture expanded, relatively homogeneous adipose-derived stromal/stem cells. The stage has been set for clinicians to translate adipose-derived cells from the bench to the bedside; however, this process will involve “development” steps that fall outside of traditional “hypothesis-driven, mechanism-based” paradigm. This concise review examines the next stages of the development process for therapeutic applications of adipose-derived cells and highlights the current state of the art regarding clinical trials. It is recommended that the experiments addressing these issues be reported comprehensively in the peer-review literature. This transparency will accelerate the standardization and reproducibility of adipose-derived cell therapies with respect to their efficacy and safety.

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“…Adipose-derived stem cells is already used for healing of oncology lesions from radiotherapy in humans, ameliorate skin flap viability and chronic skin ulcers with diabetic mice 8,11,14,15 . The focus of this study was to evaluate in acute wounds.…”

Section: Resultsmentioning

confidence: 99%

Stem cells from adipose tissue improve the time of wound healing in rats

et al. 2016

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PURPOSE:To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS:Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS:All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions.CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.

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Clinical Treatment of Radiotherapy Tissue Damage by Lipoaspirate Transplant: A Healing Process Mediated by Adipose-Derived Adult Stem Cells

Cited by 1,016 publications

References 23 publications

Mesenchymal stem cells share molecular signature with mesenchymal tumor cells and favor early tumor growth in syngeneic mice

Mesenchymal stem cells share molecular signature with mesenchymal tumor cells and favor early tumor growth in syngeneic mice

Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Stem Cells: Let's Not Get Lost in Translation

Stem cells from adipose tissue improve the time of wound healing in rats

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