Clinical Studies With Df32p on the Lifespan of Platelets

A Study of ⁵¹Cr-LabeIIed Platelets and the Chromosomal Pattern in a Case of Primary Haemorrhagic Thrombocythaemia

“…Other studies did not show this [2,18], In our patient, an abnormally short life span of normal platelets was also demonstrated. This finding seems to be a rather unspecific phenomenon as it has been found in a great many pla telet and splenic diseases [1,5,17], in venous and arterial occlusive states, uraemia, hepatic cirrhosis and other diseases.…”

“…The rationale for undertaking special studies of the platelets of this particular patient would be, that only few details are known about platelet life span in this rare disease, and the results have been very contradictory [2,7,9,13,18]. In ad dition to this the initial uptake of platelets in the reliculo-endothelial system of a patient like this has to our knowledge not been investigated.…”

Haemorrhagic Thrombocythaemia

“…Shaw and Oliver (1958) compared the platelet function in three cases of primary haemorrhagic thrombocythaemia with three cases of secondary thrombocythaemia; the primary cases showed a moderate HAEMORRHAGIC THROMBOCYTHAEMIA BRmSH MEDICAL JOURNAL impairment of platelet function, and in the secondary group there was normal function. Alfos et al (1959) found poor platelet thromboplastic function on one occasion in one of their cases. Gunz (1960) reviewed the literature on haemorrhagic thrombocythaemia and added five personal cases, in four of which thromboplastin-generation tests were carried out; in one case the platelet thromboplastic function was very deficient, and in two others it was delayed on one occasion but normal on another.…”

“…This is evident from the following papers: Stefanini andDameshek (1955), Friedman et al (1956), Levin et al (1957), Bousser (1957), Thieffry et al (1957), Goudemand and Hutin (1957), Tubiana and Boiron (1957), Fountain (1958), Benney and Lewis (1959), Alfos et al (1959), andGunz (1960).…”

⁷⁵SE-Methionine Platelet Survival Studies: a Proposal for a Mathematical Correction of the Curve

“…Two isotopic approaches have been employed so far in the study of platelet survival. The first consists in the 'cohort' labelling of platelets [10], the other consists in the 'random' labelling of a mixed circulat ing platelet population [10], Since 32P-orthophosphate and l4C-serotonin proved unsatis factory for the random labelling due to in vivo release and reutilization [8,9], the pro cedure recommended by ICSH [12] is the in vitro labelling with 5lCr [8,11,16,19,21], For the cohort labelling 32DFP [1,22], 35S and 75Se-methionine [2,14,15] have been used. 75Se-methionine, e.g., is directly incorpo rated into megakaryocytes and can be dem onstrated in the platelets after release from the bone marrow [2,7], This technique of labelling has the advantage of being easier than 5lCr labelling and of supplying informa tion on platelet maturation time.…”