Clinical Studies in Non-Dysenteric Intestinal Amebiasis

Sapero, James J.

doi:10.4269/ajtmh.1939.s1-19.497

Cited by 23 publications

(4 citation statements)

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“…69,70 Infection in all the cases where it could be determined in the cohort of CIA, (characterized by abdominal distension, colicky pain, tenesmus, alternating diarrhea and constipation, and positive serology) was due to E. dispar. Studies carried out in India, 71 Panama, 72 Puerto Rico, 73 and Honduras 74 have shown that the typical abdominal symptoms of CIA were observed more frequently in individuals negative for amoeba in feces. 75 On the other hand, individuals with diagnosis of CIA treated with anti-amoebic drugs showed no improvement of symptoms.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Sánchez-Guillén¹,

Pérez-Fuentes

Salgado-Rosas

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. To correlate a particular state of immunity with Entamoeba spp., we used colorimetric PCR to differentiate E. histolytica from E. dispar in individuals with amoebiasis and to associate its presence with the clinical profile, including humoral and cellular immune responses to E. histolytica. Our results showed high levels of antibody in acute amoebiasis and elevation of IL-4 production, a cytokine related to Th2 profile, associated with E. histolytica. In chronic amoebiasis, even with anti-E. histolytica seropositivity, intestinal symptoms were associated with E. dispar in all the cases, without differences in level of antibodies, BTI, CD4+/CD8+ ratio, INF-␥, and IL-4. Among asymptomatic carriers, E. dispar was more frequently found; however, identification of E. histolytica in two asymptomatic carriers associated with high levels of INF-␥, a cytokine related to Th1 profile, demonstrate the importance of making specific diagnosis of Entamoeba spp., to establish the clinical and epidemiological behavior in both intestinal and extra-intestinal amoebiasis.

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Section: Discussionmentioning

confidence: 99%

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Sánchez-Guillén¹,

Pérez-Fuentes

Salgado-Rosas

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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“…D. fragilis was observed in large numbers at the onset of the illness, and variations in its abundance corresponded to the severity of clinical symptoms. After several treatments with (25,35,56,72,80,99,103,104,137). Yoeli (147) described nine patients who suffered from acute intestinal signs such as explosive diarrhea, severe abdominal pain, cramps, nausea, vomiting, mild fever, and general fatigue.…”

Section: Clinical Overviewmentioning

confidence: 99%

Emerging from Obscurity: Biological, Clinical, and Diagnostic Aspects of Dientamoeba fragilis

2004

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Ever since its first description in 1918, Dientamoeba fragilis has struggled to gain recognition as a significant pathogen. There is little justification for this neglect, however, since there exists a growing body of case reports from numerous countries around the world that have linked this protozoal parasite to clinical manifestations such as diarrhea, abdominal pain, flatulence, and anorexia. A number of studies have even incriminated D. fragilis as a cause of irritable bowel syndrome, allergic colitis, and diarrhea in human immunodeficiency virus patients. Although D. fragilis is most commonly identified using permanently stained fecal smears, recent advances in culturing techniques are simplifying as well as improving the ability of investigators to detect this organism. However, there are limitations in the use of cultures since they cannot be performed on fecal samples that have been fixed. Significant progress has been made in the biological classification of this organism, which originally was described as an ameba. Analyses of small-subunit rRNA gene sequences have clearly demonstrated its close relationship to Histomonas, and it is now known to be a trichomonad. How the organism is transmitted remains a mystery, although there is some evidence that D. fragilis might be transmitted via the ova of the pinworm, Enterobius vermicularis. Also, it remains to be answered whether the two distinct genotypes of D. fragilis recently identified represent organisms with differing virulence

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“…Dientamoeba fragilis is a protozoan parasite of the human large intestine. A pathogenic role for D. fragilis has been suggested (Wenrich et al ., 1935 ; Sapero, 1939 ; Girginkardesler et al ., 2003 ; Johnson et al ., 2004 ; Stark et al ., 2010 ; Barratt et al ., 2011a ); however, there is lack of conclusive evidence of its virulence and the mechanisms involved. Although first described almost a century ago by Jepps & Dobell ( 1918 ), little is known about D. fragilis transmission modes (Barratt et al ., 2011b ).…”

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confidence: 99%

Dientamoeba fragilisDNA detection inEnterobius vermiculariseggs

et al. 2013

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Dientamoeba fragilis is an intestinal protozoan suspected of causing gastrointestinal symptoms, and its mode of transmission is unknown, although first described almost a century ago. A hypothesis is that Enterobius vermicularis is a vector for D. fragilis, and recently, D. fragilis DNA was detected within surface-sterilized eggs of E. vermicularis. Using real-time PCR, we detected D. fragilis DNA in 18 (85%) of 21 samples of E. vermicularis eggs collected from patients harbouring D. fragilis in faeces. This finding supports the hypothesis that E. vermicularis may have an important role in the transmission of D. fragilis.This paper describes a protocol to wash and surface-sterilize E. vermicularis eggs, with the aim of showing presence of both E. vermicularis and D. fragilis specific DNA within, and the results from 20 co-infected patients. The study has merit as a confirmatory study of the trials by Röser et al. (2013), and includes improvements of the protocol.

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Clinical Studies in Non-Dysenteric Intestinal Amebiasis

Cited by 23 publications

References 0 publications

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Emerging from Obscurity: Biological, Clinical, and Diagnostic Aspects of Dientamoeba fragilis

Dientamoeba fragilisDNA detection inEnterobius vermiculariseggs

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