Clinical strains ofCandida albicansexpress the surface antigen glyceraldehyde 3-phosphate dehydrogenase in vitro and in infected tissues

Abstract

We have previously described the presence of an enzymatically active form of glyceraldehyde 3-phosphate-dehydrogenase (GAPDH) in the cell surface of Candida albicans ATCC 26555 which is also a fibronectin and laminin binding protein. Immunohistochemical analysis of tissue sections from patients with disseminated candidiasis with a polyclonal antiserum to GAPDH from C. albicans (PAb anti-CA-GAPDH) revealed that the enzyme is expressed at the surface of fungal cells in infected tissues. The same PAb detected the presence of GAPDH species, with a molecular mass of approximately 33 kDa, in cell wall extracts obtained from clinical isolates of the fungus. These cell surface-bound GAPDH moieties exhibited a dose-dependent dehydrogenase activity. These results indicate that this cell surface-bound GAPDH plays a role during infection probably contributing to the attachment of fungal cells to host tissues.

Identification of GAPDH on the surface of Plasmodium sporozoites as a new candidate for targeting malaria liver invasion

“…Whereas GAP DH plays a key role in glycolysis, evidence suggests that it also can serve multiple other functions Fischetti, 1992, 1997;Alvarez-Dominguez et al, 1997;Daubenberger et al, 2003;Bergmann et al, 2004;Boël et al, 2005;Terao et al, 2006;Jin et al, 2011). In particular, GAP DH has been implicated as a ligand for cell invasion present on the surface of several pathogenic microbes and has been tested as a vaccine candidate (Gil et al, 1999;Argiro et al, 2000;Rosinha et al, 2002;Lama et al, 2009;Perez-Casal and Potter, 2016). Recent studies have identified surface expression of Figure 5.…”

Identification of the Most Immunoreactive Antigens of Candida auris to IgGs from Systemic Infections in Mice

“…Gapdh is located both in the cytoplasm and as a surface antigen in C. albicans , and in L. prolificans secretome, cell surface and hyphae. , This protein is also important for the evasion of the host immune system by high affinity binding to regulators of complement activation such as Factor H (FH) or FH-like protein (FHL-1) .…”

Potential Antifungal Targets Based on Glucose Metabolism Pathways of Candida albicans

“…Except for its significant role in the process of glycolysis in C. albicans, GAPDH has been identified as the cytosolic antigen on the cell wall surface which also is enzymatically active (Gil-Navarro et al, 1997). Gil et al (1999) and Delgado et al (2003) suggested that the cell wall-bound GAPDH expressed both on the surface of clinical isolates and on the fungal cells in infected host tissues. Generally, adhesion is associated with tissue invasive process and virulence in C. albicans (Gil et al, 1999).…”

“…Gil et al (1999) and Delgado et al (2003) suggested that the cell wall-bound GAPDH expressed both on the surface of clinical isolates and on the fungal cells in infected host tissues. Generally, adhesion is associated with tissue invasive process and virulence in C. albicans (Gil et al, 1999). Because of the ability that GAPDH in C. albicans can bind hosts' fibronectin and laminin, it contributes to the progress of adhesion during candidiasis infections (Gil et al, 1999).…”

“…Generally, adhesion is associated with tissue invasive process and virulence in C. albicans (Gil et al, 1999). Because of the ability that GAPDH in C. albicans can bind hosts' fibronectin and laminin, it contributes to the progress of adhesion during candidiasis infections (Gil et al, 1999). Moreover, GAPDH was reported to be found in the outermost layers of the cell wall, and the anti-GAPDH IgG antibody plays an important role in defining candidiasis infections (Seidler, 2013).…”