Clinical significance of urinary enzymes in diabetic nephropathy

Morita, Emiko; Kaizu, Kazo; Uriu, Kohei; Hashimoto, Osamu; Komine, Norikuni; Eto, Sumiya

doi:10.1016/0891-6632(91)90056-u

Cited by 11 publications

(6 citation statements)

References 2 publications

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“…This indicates the usefulness of urinary NAG over urinary GGT and suggests urinary NAG as better marker as predictor of diabetic nephropathy than urinary GGT. Thus our findings are in agreement with the reported data, which indicated that urinary GGT is not significant as early markers (17,18) and there is no correlation between urinary NAG and urinary GGT (12,19). However this finding is in contradiction to some reported data in which a significant decrease in urinary GGT excretion was reported in albuminuric diabetic patients (20).…”

Section: Discussionsupporting

confidence: 90%

Urinary N-acetyl beta glucosaminidase and gamma glutamyl transferase as early markers of diabetic nephropathy.

Ambade

Sing

Somani

et al. 2006

Indian J Clin Biochem

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Section: Discussionsupporting

confidence: 90%

Urinary N-acetyl beta glucosaminidase and gamma glutamyl transferase as early markers of diabetic nephropathy.

Ambade

Sing

Somani

et al. 2006

Indian J Clin Biochem

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“…Elevated urinary NAG excretion has been reported in renal damage produced by various causes, including diabetic humans and rats (33,34). In this study we found elevated NAG and other studied enzymes in the DTM2-MAA patient group.…”

Section: Discussionsupporting

confidence: 62%

The Significance of Urinary Markers in the Evaluation of Diabetic Nephropathy

Cvetković¹,

Vlahović²,

Đorđević³

et al. 2008

Journal of Medical Biochemistry

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The Significance of Urinary Markers in the Evaluation of Diabetic Nephropathy Oxidative stress is considered to be a unifying link between diabetes mellitus (DM) and its complications, including nephropathy (DN). The aim of this study was to determine the parameters of oxidative injury of lipids and proteins as well as the activity of ectoenzymes in the urine of DN patients. The study included 40 individuals: 10 patients with type 2 diabetes mellitus and microalbuminuria (DMT2-MIA), 10 type 2 diabetic patients with macroalbuminuria (DMT2-MAA), 10 patients with type 1 diabetes and microalbuminuria (DMT1-MIA) and 10 age- and sex-matched healthy subjects (control). In the urine we determined TBA reactive substances (TBARS), reactive carbonyl groups (RCG), and the activity of ectoenzymes N-acetyl-β-d-glucosaminidase (NAG), plasma cell differentiation antigen (PC-1), aminopeptidase N (APN) and dipeptidyl peptidase IV (DPP IV). A higher concentration of TBARS in the urine was found in DMT2-MIA and DMT1-MIA, compared to the control group (p<0.001 and P<0.05). The urine concentration of RCD shows similar results with a significant elevation in the groups with DMT2-MAA and DMT1-MIA, compared to the DMT2-MIA (p<0.001) and control group (p<0.001). Activities of NAG, APN and DPPIV were significantly higher in the urine of DMT2-MAA, compared to the control (p<0.01). The activity of PC-1 was slightly increased in that group, but not significantly. In conclusion, the level of oxidative stress markers and activities of brush border ectoenzymes in the urine may be a useful non-invasive and easily repeatable test in DN.

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“…That is why, in the present study, we thought to determine urinary NAG and albumin excretion as important markers showing diabetic nephropathy, because there was a contradiction in the results of classical markers of oxidative stress, especially with regard to anti‐oxidant enzymes. In diabetic patients, high urinary NAG excretion may reflect lysosomal dysfunction of both glomerular and proximal tubular epithelial cells 36 . Thus, the increase in urinary NAG excretion in our diabetic rats may imply that diabetic nephropathy involves damage to the cells of the renal tubules.…”

Section: Discussionmentioning

confidence: 78%

MELATONIN REDUCES URINARY EXCRETION OF N‐ACETYL‐β‐d‐GLUCOSAMINIDASE, ALBUMIN AND RENAL OXIDATIVE MARKERS IN DIABETIC RATS

Öktem

Özgüner

Yilmaz³

et al. 2006

Clin Exp Pharma Physio

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1. Increased oxidative stress has an important role in the pathogenesis of diabetic nephropathy. The aim of the present study was to evaluate diabetic nephropathy by determining markers of oxidative stress and the urinary excretion of N-acetyl-beta-D-glucosaminidase (NAG), albumin and to investigate the possible protective effects of in vivo melatonin on renal tubular oxidative damage in diabetic rats. 2. Twenty-six rats were randomly divided into three groups: (i) group I, control, non-diabetic rats (n = 9); (ii) group II, untreated diabetic rats (n = 8); and (iii) group III, melatonin-treated diabetic rats (n = 9). In groups II and III, diabetes developed 3 days after administration of a single dose of streptozotocin (35 mg/kg, i.p.). Thereafter, whereas the rats in group II received no treatment, rats in group III began to receive 10 mg/kg per day, i.p., melatonin for 8 weeks. Malondialdehyde (MDA), an index of lipid peroxidation, NAG and microalbumin in the urine, markers of renal tubular damage, were the parameters used for oxidative stress-induced renal injury. Superoxide dismutase (SOD), xanthine oxidase (XO) and glutathione peroxidase (GSH-Px) activities were determined to evaluate changes in the anti-oxidant status of kidney tissue. 3. In untreated diabetic rats, urinary NAG, albumin and renal MDA levels were markedly increased compared with control rats (P < 0.0001). However, these parameters were reduced in diabetic rats by melatonin treatment (P < 0.0001). Urinary excretion of NAG was positively correlated with the microalbuminuria and renal MDA levels (r = 0.8; P < 0.0001). The SOD and XO activities in the untreated diabetic group were found to be significantly higher than those of the control group (P < 0.0001). Superoxide dismutase and XO activities decreased in melatonin-treated rats compared with untreated diabetic rats (P < 0.002 and P < 0.023, respectively). However, the decrease did reach levels seen in control rats. There were no significant differences in GSH-Px activity between the three groups. 4. Therefore, on the basis of these data, we suggest that urinary NAG, albumin excretion, XO activity and MDA levels are more valuable parameters showing the degree of renal tubular injury than classical markers of oxidative stress, including SOD and GSH-Px, in diabetic rat kidneys. Melatonin has an ameliorating effect on oxidative stress-induced renal tubular damage via its anti-oxidant properties. Thus, it may be suggested that urinary NAG excretion and microalbuminuria may be important markers showing the degree of renal changes and the success of long-term treatment of renal impairment with melatonin.

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Clinical significance of urinary enzymes in diabetic nephropathy

Cited by 11 publications

References 2 publications

Urinary N-acetyl beta glucosaminidase and gamma glutamyl transferase as early markers of diabetic nephropathy.

Urinary N-acetyl beta glucosaminidase and gamma glutamyl transferase as early markers of diabetic nephropathy.

The Significance of Urinary Markers in the Evaluation of Diabetic Nephropathy

MELATONIN REDUCES URINARY EXCRETION OF N‐ACETYL‐β‐d‐GLUCOSAMINIDASE, ALBUMIN AND RENAL OXIDATIVE MARKERS IN DIABETIC RATS

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