Clinical significance of transcription factor RUNX2 in lung adenocarcinoma and its latent transcriptional regulating mechanism

Yang, Daping; Huang, Wanying; Chen, Gang; Chen, Shangwei; Yang, Jie; He, Rong‐Quan; Huang, Su-Hua; Gan, Ting‐Qing; Ma, Jie; Yang, Linjie; Song, Jianhua; Mo, Junxian; Tang, Zhong-Qing; Li, Changbo; Zhou, Hua-Fu; Kong, Jinliang

doi:10.1016/j.compbiolchem.2020.107383

Cited by 14 publications

(11 citation statements)

References 33 publications

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“…ese paraffinembedded tissues were subsequently sliced (4 µm in size) for IHC staining. e IHC staining process was elaborately explained in previous studies [22][23][24][25]. ENO1 antibodies were purchased from Abcam (Cambridge, UK).…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

“…Besides, the expression of LUSC mRNA and the clinical information of patients were downloaded from public databases. e searching strategy was referred to previous studies [23,[28][29][30][31]. is study entailed databases such as Genotype-Tissue Expression (GTEx, http://genome.ucsc.edu/), e Cancer Genome Atlas database (TCGA, http://genome.ucsc.…”

Section: Integration Analysis Of the Expression Of Eno1 Mrnamentioning

confidence: 99%

“…nlm.nih.gov/geo/query/acc.cgi), ArrayExpress, Sequence Read Archive (SRA), Oncomine, PubMed, and Google Scholar, China National Knowledge Infrastructure (CNKI), Chongqing VIP, and Chinese WanFang. e standards for inclusion and exclusion of datasets and the literature on this topic were elaborated in previous research [23].…”

Section: Integration Analysis Of the Expression Of Eno1 Mrnamentioning

confidence: 99%

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The Indication of Poor Prognosis by High Expression of ENO1 in Squamous Cell Carcinoma of the Lung

Huang

Chen

et al. 2021

Journal of Oncology

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The purpose of this study is to investigate the significance of alpha-enolase (ENO1) expression in squamous cell carcinoma of the lung (LUSC), its prognostic value, and prospective molecular mechanism. Using multiplatforms data, including in-house immunohistochemistry, in-house real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), in-house microarray, and public high-throughput data, the expression significance and prognostic role of ENO1 in LUSC tissues were analyzed comprehensively. With the combination of all eligible cases, compared with 941 non-LUSC lung tissues, ENO1 was significantly overexpressed in 1163 cases of LUSC (standardized mean difference (SMD) = 1.23, 95% confidence interval (CI) = 0.76–1.70, P < 0.001). ENO1 also displayed a great ability to differentiate LUSC tissues from non-LUSC lung tissues (AUC = 0.8705) with the comprehensive sensitivity being 0.88 [0.83–0.92], and comprehensive specificity being 0.89 [0.84–0.94]). Moreover, in 1860 cases of LUSC with survival information, patients with higher expression of ENO1 had poorer prognosis (hazard ratio (HR) = 1.20, 95% CI = 1.01–1.43, P = 0.043). ENO1 and its related genes mainly participated in the pathways of cell division and proliferation. In conclusion, the upregulation of ENO1 could affect the carcinogenesis and unfavorable outcome of LUSC.

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Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Section: Integration Analysis Of the Expression Of Eno1 Mrnamentioning

confidence: 99%

Section: Integration Analysis Of the Expression Of Eno1 Mrnamentioning

confidence: 99%

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The Indication of Poor Prognosis by High Expression of ENO1 in Squamous Cell Carcinoma of the Lung

Huang

Chen

et al. 2021

Journal of Oncology

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“…It was demonstrated that RUNX2 expression level was upregulated in lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) tissues. 16,17 Moreover, RUNX2 overexpression could promote EMT and increase the expression of EMT related genes including TWIST1, SNAIL1 and VIMENTIN, which directly reflect an increased migratory and invasion capacity of LUAD cells. 18 These results suggested that RUNX2 could serve as a candidate target for LC therapy.…”

Section: Introductionmentioning

confidence: 99%

RUNX2 as a Potential Prognosis Biomarker and New Target for Human Lung Cancer

Xiao¹,

Liu²,

Chen³

et al. 2021

Exploratory Research and Hypothesis in Medicine

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Background and objectives:The role of runt-related transcription factor 2 (RUNX2) in tumorigenesis and tumor progression in many cancers has been identified except for lung cancer. This study aimed to explore the expression level, functions and prognostic values of RUNX2 in lung cancer.Methods: ONCOMINE was utilized to compare RUNX2 expression difference between cancers and normal tissue. GEPIA was used to further detect the expression pattern of RUNX2 between lung cancer and corresponding normal lung tissue. Then, the prognosis value of RUNX2 in lung cancer was evaluated through Kaplan-Meier Plots. The genomic alterations of RUNX2 neighbor genes were analyzed by cBioPortal, and GO enrichment was conducted to identify functional categories of RUNX2 and genes associated with RUNX2 alterations.Results: ONCOMINE and GEPIA revealed the upregulated expression level of RUNX2 in lung cancer compared to normal lung tissue. Higher RUNX2 level was associated with lower progression-free survival and overall survival in lung cancer patients. GO enrichment implicated that cell proliferation, invasion and metastasis-related genes as well as the functions of cell adhesion, cell migration, and cell proliferation were significantly associated with RUNX2 alterations. Conclusions:Our findings indicated the value of RUNX2 as a novel prognostic biomarker and a potential therapeutic target for lung cancer.

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“…6 The detailed information on Runx2 in the regulation of pathogenicity are summarized in Table 1. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] Of interest, some factors could influence the expression of Runx2, which include: (1) microRNAs. The deletion of the micro-RNA processing enzyme Dicer leads to decreased expression of miRNAs and Runx2, which suggests a critical role for microRNA in the regulation of Runx2.…”

mentioning

confidence: 99%