Clinical significance of the RT‐PCR positive sentinel node in melanoma

Temple, Claire; Snell, Laura; Power, Stephanie; Parfitt, Jeremy; Scilley, Christopher; Engel, Christoph; Shum, David T.; Chakrabarti, Subrata; Joseph, Mariamma; Lohmann, Reinhard; Ainsworth, Peter

doi:10.1002/jso.20741

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…Along with histopathological analysis, many centers also perform studies using other techniques such as RT‐PCR, for the molecular detection of melanocytic markers, and immunocytology for the detection of melanoma cells [44, 46, 47]. In these techniques, the sentinel lymph node is cut in half, i.e., histopathological analysis is performed on half a node.…”

Section: Resultsmentioning

confidence: 99%

Histopathological diagnostics of malignant melanoma in accordance with the recent AJCC classification 2009: Review of the literature and recommendations for general practice

Garbe

Eigentler

Bauer

et al. 2011

J Deutsche Derma Gesell

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Section: Resultsmentioning

confidence: 99%

Histopathological diagnostics of malignant melanoma in accordance with the recent AJCC classification 2009: Review of the literature and recommendations for general practice

Garbe

Eigentler

Bauer

et al. 2011

J Deutsche Derma Gesell

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“…The prognostic significance of PCR analysis of melanocyte‐associated markers in melanoma SLNs is controversial (4–12, 17–25). However, a recently published meta‐analysis has concluded that PCR status of the SLNs appears to have a clinically valuable prognostic power in patients with melanoma (26).…”

Section: Discussionmentioning

confidence: 99%

“…Since these molecular techniques examine all the tissue submitted, they may be more sensitive than standard histopathological methods for detecting metastases. Indeed, a number of PCR‐based studies have shown significantly higher positivity rates for melanoma SLNs compared with traditional methods of analysis, with up to 70% of nodes positive for melanocyte markers (3–12). However, these molecular detection rates are also much higher than would be expected if PCR detects metastases alone, since the natural history of melanoma suggests that only up to 30% of stage I and stage II melanoma patients are likely to develop lymph node metastases (13).…”

mentioning

confidence: 99%

Quantitative real‐time RT‐PCR in sentinel lymph nodes from melanoma patients

Riber-Hansen

Abrahamsen

Sørensen

et al. 2008

APMIS

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Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) for specific melanoma markers is more sensitive than histology for detecting cells of melanocytic origin in sentinel lymph nodes (SLNs) in cutaneous melanoma. The clinical significance of a positive qRT-PCR analysis is unclear. We performed qRT-PCR for the presence of MART-1 and tyrosinase in SLNs from 93 melanoma patients, and then followed these patients clinically (median follow-up time 43.5 months). We found a significant correlation between disease progression and presence of MART-1 mRNA in SLNs (p=0.02), but no correlation with the amount of MART-1 mRNA as measured by qRT-PCR. No correlation between histology and recurrence was detected, recurrence rates being low in both histology-negative (12%) and -positive (15%) patients. We found a significant difference in disease recurrence between patients positive by both histology and RT-PCR and patients negative by both methods (15% vs 0%, p=0.02). However, a significant difference in disease recurrence was also found when comparing patients negative by both methods with patients positive by RT-PCR but negative by histology (0% vs 19%, p=0.009). This suggests that the presence of submicroscopic metastases may influence prognosis, indicating that RT-PCR detection of melanocytic cells in SLNs may be an important diagnostic marker.

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“…The retrotranscription reaction was performed with 2 mg of RNA that was isolated using TRIZOL reagent Primers for tyrosinase were TYR-F: TTG GCA GAT TGT CTG TAG CC; TYR-R: AGG CAT TGT GCA TGC TGC TT; 284 bp, 35 cycles of 1 min at 951C; 1 min at 551C; 1 min at 721C [16][17][18]. Water instead of cDNA and MCF-7 cell line was used as negative controls.…”

Section: Reverse-transcription-pcrmentioning

confidence: 99%

Role of H4 receptor in histamine-mediated responses in human melanoma

et al. 2011

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We have previously reported that histamine at micromolar concentrations reduces the proliferation of melanoma cell lines. It is also known that melanoma cells express histamine H 1 , H 2 , and H 3 receptors. The aim of this study was to investigate the presence of histamine H 4 receptor (H 4 R) in human melanoma cells and its associated biological processes. To better understand the importance of histamine in tumor development, we explored the expression of H 4 R in human melanoma tissue biopsies. The expression of H 4 R in WM35 and M1/15 cells was analyzed by reverse-transcription-PCR, western blot, and immunocytochemistry. To characterize the biological responses we evaluated cell proliferation by clonogenic assay and 5-bromo-2 0 -deoxyuridine incorporation. In addition, cell senescence and differentiation were determined by b-galactosidase enzyme assay and dopa oxidase activity, respectively. The expression levels of H 4 R were determined by immunohistochemistry in 19 samples of human malignant lesions. Results indicate that melanoma cells express H 4 R at the messenger RNA and protein levels. By using histamine agonists, antagonists, and H 4 R small-interfering RNA we showed that the inhibitory effect of histamine on proliferation was in part mediated through the stimulation of the H 4 R. The decrease in proliferation was associated with an induction of cell senescence and an increase in melanogenesis, which is a differentiation marker of these cells. Furthermore, H 4 R was expressed in 42% of human melanoma biopsies. To our knowledge, this is the first report that describes the presence of the H 4 R in melanoma cells and tissue, suggesting a potential therapeutic application of H 4 R ligands.

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Clinical significance of the RT‐PCR positive sentinel node in melanoma

Abstract: Sentinel node RT-PCR positivity did not risk stratify histologically negative melanoma patients beyond routine pathologic examination in this series.

Cited by 7 publications

References 48 publications

Histopathological diagnostics of malignant melanoma in accordance with the recent AJCC classification 2009: Review of the literature and recommendations for general practice

Histopathological diagnostics of malignant melanoma in accordance with the recent AJCC classification 2009: Review of the literature and recommendations for general practice

Quantitative real‐time RT‐PCR in sentinel lymph nodes from melanoma patients

Role of H4 receptor in histamine-mediated responses in human melanoma

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