Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis

Pugliese, Michela; Sfacteria, Alessandra; Oliva, Gætano; Falcone, Annastella; Gizzarelli, Manuela; Passantino, Annamaria

doi:10.3390/ani11030754

Cited by 4 publications

(6 citation statements)

References 66 publications

(36 reference statements)

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“…The thiol deficiency is described in various viral and bacterial diseases; conversely, their activity seems to be increased during the course of parasitic diseases sustained by protozoa such as Leishmania spp. [ 9 ]. Probably, the difference mentioned above is related to different mechanisms involved in the determinism of diseases.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of concurrent infection with other vector-borne pathogens or co-morbidities in dogs is considered by clinicians as infrequent or uncharacteristic disease signs. It has been suggested that oxidative stress may play a central function in the onset of clinical signs in the course of CME [ 7 , 8 ], as well as in another vector-borne disease, canine leishmaniasis [ 9 ]. Previous studies performed in experimental conditions have demonstrated a status of oxidative stress in dogs infected by Ehrlichia canis [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

et al. 2022

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The study aimed to evaluate the concentration of reactive oxidative metabolites (R-OOHs), the antioxidant barrier (OXY), and the ratio between R-OOHs and OXY (OSi) and thiol groups of plasma compounds (SHp) in in canine monocytic ehrlichiosis. Thirty dogs affected with monocytic ehrlichiosis (canine monocytic ehrlichiosis group—CME group) and ten healthy dogs (control group—CTR group) were evaluated. CME was diagnosed by the presence of clinical signs and the detection of anti-Ehlichia canis antibodies. Oxidative stress parameters of two groups were compared using the Mann–Whitney test. Significance was set at p < 0.05. Spearman rank correlation was performed to analyze oxidative stress, and hematological and biochemical variables in the CME group. All dogs affected with CME showed a wide spectrum of clinical signs such as lethargy, anorexia, fever, weight loss, lymph adenomegaly, splenomegaly, subcutaneous and mucosal petechial and ecchymosis, and vomiting. Anaemia, leukocytosis, thrombocytopenia, hyperglobulinemia, hypoalbuminemia and an increase of blood urea nitrogen and creatinine are also detected. Results showed significantly lower values of SHp in the CME group than in CTR. A statistically significant difference in the number of white blood cells, platelets, and blood urea nitrogen concentration was assayed comparing to the two groups. A negative correlation between SHp and hemoglobin concentration was recorded. These preliminary results may suggest a possible function of oxidative stress in the onset of clinical signs during the course of CME.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

et al. 2022

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“…The intensity of the colored complex was inversely correlated with the total antioxidant capacity. A surplus of hypochlorous acid radicals present after the oxidative reaction indicated a reduction in the plasma barrier; conversely, a reduction in the values was directly correlated with an injury of the plasma barrier due to oxidation [ 24 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

“…High-mobility group box 1 (HMGB-1) is a nuclear protein that is involved as a fundamental co-factor in transcriptional regulation, and that is secreted into the extracellular environment following an apposite stimulus. Extracellular HMGB-1 acts as an alarmin that contributes to the intensification of inflammatory responses, thus interrelating with endothelial cells and triggering the release of pro-inflammatory mediators [ 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. Moreover, the central role of HMGB-1 in response to oxidative stress in humans has been documented in several pathological conditions [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress and High-Mobility Group Box 1 Assay in Dogs with Gastrointestinal Parasites

et al. 2022

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This study aimed to evaluate the concentration of reactive oxidative metabolites, the antioxidant barrier, thiol groups of plasma compounds, and high-mobility group box 1 in shelter dogs naturally infected with helminths. In addition, the correlation between clinical signs and oxidative stress was investigated. Sixty-six (41 male and 25 female) adult mixed-breed dogs housed in a shelter with the diagnosis of gastrointestinal nematodes (i.e., Ancylostoma spp., Uncinaria stenocephala, Toxocara canis, Toxascaris leonina, or Trichuris vulpis) were enrolled in Group 1 (G1) and twenty healthy adult dogs were included in Group 2 (G2), which served as the control. A clinical assessment was performed using a physician-based scoring system. Oxidative stress variables and high-mobility group box 1 were assessed and compared by the means of unpaired t-tests (p < 0.05). Spearman’s rank correlation was performed to calculate the correlation between oxidative stress variables, high-mobility group box 1, hematological parameters, and clinical signs. The results showed statistically significant values for reactive oxidative metabolites, thiol groups of plasma compounds, and high-mobility group box 1 in G1. Negative correlations between thiol groups and the number of red cells and hemoglobin were recorded. These preliminary results support the potential role of oxidative stress and HGMB-1 in the pathogenesis of gastrointestinal helminthiasis in dogs.

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“…Many studies, including CanVL (6,7,10,11,33,62,63), claim that reactive oxygen species (ROS) play a role in the pathogenesis of various infectious and parasitic diseases in dogs (14,23,37,40,59,70). Excessive ROS production leads to oxidative stress as a result of imbalance between oxidants and antioxidants.…”

Section: Investigation Of Dna Damage and Protein Damage Caused By Oxidative Stress In Canine Visceral Leishmaniasismentioning

confidence: 99%

Investigation of DNA damage and protein damage caused by oxidative stress in canine visceral leishmaniasis

Kiral¹,

Sekkin²,

Paşa³

et al. 2021

Medycyna Weterynaryjna

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Leishmaniasis, considered by the World Health Organization as one of the most important zoonotic diseases, causes death when it is not treated in its self-healing skin form. The aim of this study was to investigate DNA damage and oxidative protein damage that occurs in dogs infected with Leishmania infantum. The study group consisted of 25 dogs, including 10 clinically healthy dogs aged 2 to 6 years and 15 dogs infected with Leishmania infantum diagnosed by means of the rk39 dipstick test and the immunofluorescence antibody test (IFAT). The effects of oxidative stress on protein were evaluated in the dogs infected with Leishmania infantum by determining plasma malondialdehyde, protein carbonyl groups, nitrotyrosine, and total antioxidant capacity in blood. DNA damage, on the other hand, was determined by the COMET method. Plasma protein carbonyl content (PCO) and nitrotyrosine (NT) levels, which are considered as indicators of protein damage, were found to be higher in the dogs infected with Leishmania infantum compared to the control group, but the difference in both values was not statistically significant (p > 0.05). Plasma malondialdehyde (MDA), an indicator of lipid peroxidation, was significantly higher in the dogs infected with Leishmania infantum than in the control group. TAC levels, however, were lower in the leishmanial dogs (p < 0.05). According to the results of the COMET assay, lymphocyte cells were damaged in the leishmanial dogs, and both tail intensity (TI) and tail moment (TM) values were higher in those dogs than they were in the control group (p < 0.05, p < 0.01). The parasites caused oxidative stress through protein and DNA damage in the host and decreased the antioxidant capacity concentration that prevents destructive effects.

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Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis

Cited by 4 publications

References 66 publications

Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

Oxidative Stress and High-Mobility Group Box 1 Assay in Dogs with Gastrointestinal Parasites

Investigation of DNA damage and protein damage caused by oxidative stress in canine visceral leishmaniasis

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