Clinical significance of protocadherin 8 (PCDH8) promoter methylation in non-muscle invasive bladder cancer

Lin, Ying‐Li; Wang, Yanling; Ma, Jianguo

doi:10.1186/preaccept-1759254111327153

Cited by 1 publication

(4 citation statements)

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“…Members of the protocadherin family demonstrated various functions from tumor suppression to tumor promotion . PCDH8 was found to be epigenetically silenced in various cancers . Zhang and colleagues restored PCDH8 expression by treating cells with 5‐aza‐2′‐deoxycytidine, a reagent that causes demethylation, and found proliferation and migration of cells to be suppressed .…”

Section: Discussionmentioning

confidence: 99%

“…1 PCDH8 was found to be epigenetically silenced in various cancers. [3][4][5][6][7] Zhang and colleagues restored PCDH8 expression by treating cells with 5-aza-2 0 -deoxycytidine, a reagent that causes demethylation, and found proliferation and migration of cells to be suppressed. 7 However, we should not ignore the fact that demethylation by 5-aza-2 0 -deoxycytidine was not gene specific such that not only PCDH8 but also many other genes were demethylated.…”

Section: Discussionmentioning

confidence: 99%

“…Protocadherin‐8 ( PCDH8 ) is another member of the protocadherin family. Methylation of PCDH8 was found to be related to poor prognosis or unfavorable clinicopathological features in various cancers, including gastric cancer (GC) . Exogenous expression of PCDH8 inhibited proliferation and migration in breast cancer cells and nasopharyngeal cancer cells .…”

Section: Introductionmentioning

confidence: 99%

“…Methylation of PCDH8 was found to be related to poor prognosis or unfavorable clinicopathological features in various cancers, including gastric cancer (GC). [3][4][5][6][7] Exogenous expression of PCDH8 inhibited proliferation and migration in breast cancer cells and nasopharyngeal cancer cells. 3,6 Re-expression of PCDH8 in GC cells using demethylation reagent inhibited migration and induced apoptosis in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Protocadherin‐8 promotes invasion and metastasis via laminin subunit γ2 in gastric cancer

Lin

Zhang

et al. 2018

Cancer Science

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Growing evidence suggests that protocadherins (PCDH) play crucial roles in pathogenesis and progression of cancers, including gastric cancer (GC). Protocadherin‐8 (PCDH8) was previously reported to be involved in metastasis of GC, but functional studies yielded inconsistent results and the molecular mechanism remained unknown. The present study aimed to explore the clinical relevance, function and molecular mechanism of PCDH8 in GC. Data from the GEPIA and Kaplan–Meier plotter databases showed that high expression of PCDH8 was significantly correlated with poorer prognosis in GC. Ectopic expression of PCDH8 in GC cells promoted invasion and migration in vitro and metastasis in vivo, and knockdown of PCDH8 inhibited invasion and migration in vitro. RNA sequencing followed by gene set enrichment analysis found a remarkable enrichment in the extracellular matrix receptor interaction pathway, with the expression of laminin subunit γ2 (LAMC2) being significantly increased in the PCDH8‐overexpressing group. High expression of LAMC2 was significantly correlated to poor prognosis in GC in GEPIA database. Upregulation of LAMC2 following PCDH8 overexpression was further confirmed by immunohistochemistry in liver metastatic lesions of nude mice. To our knowledge, this is the first report of the metastasis‐enhancing property and molecular mechanism through upregulation of LAMC2 of PCDH8 in cancer. High expression of PCDH8 could be used as a biomarker for poor prognosis in clinical practice.

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Section: Discussionmentioning

confidence: 99%