Clinical Significance of Peripheral Blood T Lymphocyte Subsets inHelicobacter pylori-Infected Patients

Abstract

Background. Helicobacter pylori chronically colonizes gastric/duodenal mucosa and induces gastroduodenal disease and vigorous humoral and cellular immune responses. Methods. In order to clarify the immunological changes induced by this infection, we determined the percentage and, as indicated, ratios of the following cells in peripheral blood of 45 H. pylori-infected patients and 21 control subjects: CD4+ T cell, CD8+ T cells, T helper 1 cells (Th1), T helper 2 cells (Th2), CD4+CD25+ T cells, Foxp3+ regulatory T cells (Tregs), CD4/CD8 ratio, and Th1/Th2 ratio. Results. The percentage of CD8+ T cells was significantly lower in H. pylori-infected patients (mean ± SD; 18.0 ± 7.1%) compared to control subjects (mean ± SD; 23.2 ± 7.8%) (P < 0.05). The CD4/CD8 ratio was significantly higher in H. pylori-infected patients (mean ± SD; 3.1 ± 2.4) compared to control subjects (mean ± SD; 2.1 ± 1.0) (P < 0.05). The Th1/Th2 ratio was significantly lower in H. pylori-infected patients (mean ± SD; 10.0 ± 8.5) compared to control subjects (mean ± SD; 14.5 ± 9.0) (P < 0.05). The percentage of CD4+CD25+ T cells in H. pylori-infected patients (mean ± SD; 13.2 ± 6.2%) was significantly higher than that in control subjects (mean ± SD; 9.8 ± 3.4%) (P < 0.05). However, there was no significant difference in Tregs. Conclusion. Tregs did not decrease, but the activation of humoral immunity and Th2 polarization were observed in the peripheral blood of H. pylori-infected patients. In some cases, these changes may induce systemic autoimmune diseases.

Characterization and Functional Properties of Gastric Tissue-Resident Memory T Cells from Children, Adults, and the Elderly

“…Therefore, there was an inversion in the CD4/CD8 ratio in LPMC (~1:3) compared to PBMC (~3:1). These results confirmed and extended those reported by others (16, 19, 20) by showing that these differences were observed in all age groups (Figure 3B). When CD4 + T cells in LPMC of children and the elderly were compared to adults, children showed a significantly higher frequency of these cells (Figure 3B).…”

Regulatory T cells percentage in peripheral blood before and after eradication of &lt;i&gt;Helicobacter pylori&lt;/i&gt;

“…Yuceyar et al reported that there was no significant difference in peripheral blood lymphocyte subsets between H. pylori-infected patients and control subjects [7], but we have reported a decrease in the percentage of CD8+ T cells and an increase in the CD4/CD8 ratio in peripheral blood lymphocytes of H. pylori-infected patients [9]. We suspected that the distribution of CD8+ T cells changes in H. pylori-infected patients, with migration of peripheral CD8+ T cells into the gastric mucosa, based on the reports that showed CD8+ T cell accumulation in the gastric mucosa, which increases the severity of gastric inflammation.…”

“…Given this association with autoimmune diseases, we hypothesized that H. pylori might induce systemic immunological changes. Therefore, we compared the immune characteristics of peripheral blood of H. pylori-infected patients and control subjects, and we reported activation of humoral immunity and Th2 polarization in H. pylori-infected patients [9]. Based on these data, we suspected that these abnormalities may induce systemic autoimmune diseases.…”

“…We have reported that an increased CD4/CD8 ratio and a decreased Th1/Th2 ratio were observed in H. pylori-infected patients. Based on these data, we concluded that the activation of humoral immunity and Th2 polarization in peripheral blood were characteristic features of H. pylori-infected patients [9]. In this study, the peripheral blood T cell subsets before and after eradication of H. pylori were analyzed to determine the influence of H. pylori infection on the systemic immune response.…”

Peptic ulcer as a risk factor for postherpetic neuralgia in adult patients with herpes zoster

“…The major risk factors of peptic ulcer are Helicobacter pylori ( H. pylori ) infection and usage of non‐selective nonsteroidal anti‐inflammatory drugs (nsNSAIDs), accounting for 48% and 24% of cases, respectively in the United States [Ramakrishnan and Salinas, ]. H. pylori infection is reported to be associated with immunological dysregulation and malnutrition [Salgueiro et al, ; Gerhard et al, ; Aditi and Graham, ; Satoh et al, ]. Complications of nsNSAIDs usage include impaired cell‐mediated immunity [Paccani et al, ; Kim et al, ], chronic protein/blood loss [Bjarnason et al, ] and antioxidant/nutrient deficiencies [Nair et al, ; Biondi et al, ].…”