Clinical significance of microRNA-148a in patients with early relapse of stage II stage and III colorectal cancer after curative resection

Tsai, Hsiang‐Lin; Yang, I‐Ping; Huang, Ching‐Wen; Ma, Cheng‐Jen; Kuo, Chao‐Hung; Lu, Chien‐Yu; Juo, Suh‐Hang Hank; Wang, Jaw‐Yuan

doi:10.1016/j.trsl.2013.07.009

Cited by 50 publications

(48 citation statements)

References 43 publications

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“…It has been reported that miR-148a downregulation is a statistically significant prognostic factor in both stage III/IV and stage II/III CRC cohorts [15,22]. In this study, we intended to determine the significance of miR-148a downregulation in CRC cases without any stage limitation, but it was finally found that its downregulation was an independent prognostic factor only in patients with stage III CRC.…”

Section: Discussionmentioning

confidence: 96%

Significance of miR-148a in Colorectal Neoplasia: Downregulation of miR-148a Contributes to the Carcinogenesis and Cell Invasion of Colorectal Cancer

Hibino¹,

Sakamoto²,

Naito³

et al. 2015

Pathobiology

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Objective: Colorectal cancer (CRC) develops through the deregulation of gene expression and the accumulation of epigenetic abnormalities, leading to tumor cell acquisition of malignant features. MicroRNAs (miRNAs) play a critical role in cancer development, where they can act as oncogenes or oncosuppressors. Methods: miR-148a expression was measured by qRT-PCR in patients with colorectal adenoma (n = 21) and CRC (stage I-IV, n = 159) using formalin-fixed paraffin-embedded tissue samples. In situ hybridization (ISH) using an miR-148a-specific probe was also performed. To further confirm the direct effect of miR-148a on matrix metalloproteinase (MMP)7 expression in CRC, MTT and cell invasion assays using HT29 and WiDr cells were performed. Results: miR-148a expression was found to be clearly downregulated in high-grade adenoma compared to low-grade adenoma on both qRT-PCR and ISH analysis. Downregulation of miR-148a expression was significantly correlated with advanced clinicopathological features and was an independent prognostic classifier in patients with stage III CRC. In CRC cells and tissues, miR-148a expression was inversely correlated with the expression of MMP7. Conclusion: We showed the collaborative participation of miR-148a and MMP7 in CRC cell invasion. These results also demonstrate that the downregulation of miR-148a expression promotes CRC progression, especially carcinogenesis and cancer cell invasion.

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Section: Discussionmentioning

confidence: 96%

Significance of miR-148a in Colorectal Neoplasia: Downregulation of miR-148a Contributes to the Carcinogenesis and Cell Invasion of Colorectal Cancer

Hibino¹,

Sakamoto²,

Naito³

et al. 2015

Pathobiology

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“…However, there were limited numbers of reports about miRNA expression in stage II CRC. For example, miR-21 [32, 33], miR-29a [18], miR-34a-5p [34], miR-148a [35], and others [36, 37] were reported as predictive factors for recurrence in stage II CRC patients. Recently, six-miRNA-based classifier, which contained miR-21-5p, -20a-5p, -103a-3p, -106b-5p, -143-5p, and -215, had been reported as a prognostic and a predictive tool for disease recurrence in stage II CRC patients [38].…”

Section: Discussionmentioning

confidence: 99%

High expression of miR-181c as a predictive marker of recurrence in stage II colorectal cancer

et al. 2016

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INTRODUCTIONA standard treatment for stage II colorectal cancer (CRC) is surgical resection without adjuvant chemotherapy. However, the recurrence rate of these patients is approximately 20%. To date, there are no robust biomarkers suitable for predicting recurrence in stage II CRC patients. In this study, microRNAs (miRNAs) extracted from CRC tissues were examined for a possible biomarker to predict recurrence in stage II CRC patients.RESULTSFrom the comprehensive analysis, 15 miRNAs were selected as candidates for further study. Regarding let-7a, -7d, -7e, miR-23c, -26b, -128a, -151-5p, and -181c, recurrence rates in training cohort patients with higher expression of these miRNAs isolated from their frozen tissues samples were significantly higher than those with lower expression (P < 0.05). According to multivariate analysis, the higher expression of miR-181c was detected as an independent predictive factor of recurrence (P = 0.001, OR: 9.43, 95% CI: 2.57–34.48). Results were similar in miR-181c extracted from FFPE tissues obtained from the training cohort (P = 0.003, OR: 7.46, 95% CI: 1.97–28.57). In the validation cohort using FFPE tissues, the recurrence rate in patients with higher miR-181c expression was significantly higher than those with lower miR-181c expression (P < 0.001).MATERIAL AND METHODSComprehensive analysis using a highly sensitive miRNA chip was initially performed to select candidate miRNAs associated with recurrence. Candidate miRNAs were analyzed by real-time RT-PCR using RNA from frozen and formalin-fixed, paraffin-embedded (FFPE) tissues.CONCLUSIONSHigher expression of miR-181c may be a useful recurrence predictor of stage II CRC patients.

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“…30 For the liver they have identified the expression of miRNA-122a, for the heart miRNA-1 and miRNA-133a, for the brain miRNA-9, for the pancreas miRNA-216, for the lungs miRNA-223, for testis miR-NA-204, for bone marrow miRNA-223, and for skeletal muscle miRNA-1 and miRNA-133°. [30][31][32][33][34][35] …”

Section: Specificity Of Mirnas For Biological Fluids and Tissuesmentioning

confidence: 99%

Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Dumache

Rogobete

Săndesc

et al. 2017

Journal of Interdisciplinary Medicine

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The current practice in the field of forensic medicine imposes the use of modern investigation techniques. The complexity of laboratory investigation methods needed for a final result of the investigation in forensic medicine needed new biomarkers of higher specificity and selectivity. Such biomarkers are the microRNAs (miRNAs), short, non-coding RNAs composed of 19-24 nucleotides. Their characteristics, such as high stability, selectivity, and specificity for biological fluids, differ from tissue to tissue and for certain pathologies, turning them into the ideal candidate for laboratory techniques used in forensic medicine. In this paper, we wish to highlight the biochemical properties and the usefulness of miRNAs in forensic medicine.

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Clinical significance of microRNA-148a in patients with early relapse of stage II stage and III colorectal cancer after curative resection

Cited by 50 publications

References 43 publications

Significance of miR-148a in Colorectal Neoplasia: Downregulation of miR-148a Contributes to the Carcinogenesis and Cell Invasion of Colorectal Cancer

Significance of miR-148a in Colorectal Neoplasia: Downregulation of miR-148a Contributes to the Carcinogenesis and Cell Invasion of Colorectal Cancer

High expression of miR-181c as a predictive marker of recurrence in stage II colorectal cancer

Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

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