Clinical significance of hsa_circ_0000419 in gastric cancer screening and prognosis estimation

Xu, Tao; Shao, Yongfu; Lu, Rongdan; Ye, Qihua; Xiao, Bingxiu; Guo, Junming

doi:10.1016/j.prp.2019.152763

Cited by 37 publications

(39 citation statements)

References 28 publications

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“…The expression level of hsa_circ_0067582 is significantly downregulated in GC tissues 19 . Furthermore, hsa_circ_0000419 is lowly expressed in both GC tissues and plasma samples, making it a potential biomarker in GC screening and predictor of patient prognosis 20 . These results strongly indicate that circRNAs play important roles in the pathophysiology of cancer and may serve as new diagnostic biomarkers and therapeutic targets.…”

Section: Discussionmentioning

confidence: 96%

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CircHN1 affects cell proliferation and migration in gastric cancer

Zhang

Wang

Zang

et al. 2020

Clinical Laboratory Analysis

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Background Increasing evidence indicates that circular RNAs (circRNAs) are dysregulated in human cancers. The biological roles of circRNAs in gastric cancer (GC) have not been well‐characterized. Methods The GEO database was used to analyze circRNA expression profile in GC. The expression level of target circRNA in tumor tissues and adjacent non‐tumor tissues was detected by reverse transcription‐quantitative PCR. Gene transfection was used to manipulate the expression of circRNAs. The biological roles of circRNAs in cell proliferation, migration, and invasion were determined by cell counting, colony formation, transwell migration, Matrigel invasion, and mouse xenograft tumor assays. The interactions between circRNAs and miRNAs were verified by RNA immunoprecipitation and luciferase reporter assays. Results We found that circHN1 was upregulated in GC tissues and cell lines compared to adjacent non‐tumor tissues and normal gastric epithelial cells. Additionally, circHN1 silencing significantly promoted GC cell growth, colony formation, migration, and invasion, whereas circHN1 overexpression had the opposite effects. CircHN1 overexpression also suppressed gastric cancer growth in the mouse xenograft tumor model. CircHN1 was mainly localized in the cytoplasm of GC cells and could bind to AGO2. MiR‐1248 and miR‐375 were predicted to interact with circHN1 by bioinformatic analyses. MiR‐1248 and miR‐375 overexpression inhibited the activity of the circHN1 luciferase reporter. Conclusion CircHN1 is aberrantly expressed in GC and affects the proliferation and migration of gastric cancer cells by acting as miRNA sponge.

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Section: Discussionmentioning

confidence: 96%

“…The roles of circRNAs in cancer biology have attracted wide attention 16 . Recent studies have demonstrated that some circRNAs are abnormally expressed in GC and involved in the development and progression of GC 17‐20 . CircNRIP1 promotes GC progression by sponging miR‐149‐5p and regulating the AKT1/mTOR pathway 17 .…”

Section: Discussionmentioning

confidence: 99%

CircHN1 affects cell proliferation and migration in gastric cancer

Zhang

Wang

Zang

et al. 2020

Clinical Laboratory Analysis

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“…9,10,12 In recent years, a series of studies have shown that circRNAs can be used as biomarkers for the diagnosis of tumors. [35][36][37] In this study, we selected circ_0004771 for subsequent study by combining the circRNA sequencing of three pairs of GC tissues and circRNA-related databases. Due to the lack of efficient and non-invasive biomarkers and many circRNAs can be detected in human body fluids, we found the expression level of circ_0004771 in plasma was higher than that in healthy controls.…”

Section: Discussionmentioning

confidence: 99%

<p>Comprehensive Assessment of Plasma Circ_0004771 as a Novel Diagnostic and Dynamic Monitoring Biomarker in Gastric Cancer</p>

Yan

Kong

Qin

et al. 2020

OTT

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Due to the lack of specific and sensitive detection indicators, most patients with GC are already in the advanced stage at the time of diagnosis. Therefore, it is urgent to search for effective diagnostic biomarkers that can be applied in clinic. Materials and Methods: We screened out circ_0004771 through circRNA sequencing. Exonuclease digestion assay, agarose gel electrophoresis (AGE) and Sanger sequencing verified the potential of circ_0004771 being a biomarker. Additionally, we established quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) to detect the expression level of circ_0004771 and evaluated the methodology. What's more, we collected plasma samples from 120 GC patients, 40 superficial gastritis patients, 20 postoperative GC patients, 20 postoperative recurrence patients and 120 healthy donors. We constructed the receiver operating characteristic curve (ROC) to appraise its diagnostic efficacy. Results: The expression level of circ_0004771 is up-regulated in GC tissues, which is consistent with circRNA sequencing result (P=0.0001). Circ_0004771 can serve as a promising biomarker because of its stable structure and longer half-life. Plasma circ_0004771 expression is markedly richer in GC patients than that in normal people (P<0.0001), and the area under the ROC (AUC) is 0.831 (95% CI: 0.779-0.883). The diagnostic efficacy of circ_0004771 is higher than that of CEA (AUC=0.747, 95% CI: 0.686-0.808) and CA199 (AUC=0.508, 95% CI: 0.433-0.583). Higher diagnostic efficacy can be achieved by combination diagnosis for distinguishing GC patients from normal people (AUC=0.864). Besides, the expression level of circ_0004771 can distinguish GC patients from gastritis patients (AUC=0.845, 95% CI: 0.772-0.917). The plasma circ_0004771 expression in GC patients decreased to normal after surgery (P<0.0001). In addition, plasma circ_0004771 expression increased again in patients with postoperative recurrence. Conclusion: Plasma circ_0004771 is differentially expressed in GC patients, postoperative GC patients and patients with recurrence, suggesting that plasma circ_0004771 can be used as a novel diagnostic and dynamic monitoring biomarker in GC.

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“…In recent years, a series of studies have shown that circRNAs can be used as biomarkers for the diagnosis of tumors [27][28][29]. In this study, we selected circ_0004771 for subsequent study by combining the circRNA sequencing of three pairs of GC tissues and circRNA-related databases.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Assessment of Plasma-Derived circ_0004771 as a Potential Biomarker in Tracking the Progression of Gastric Cancer

Kong

Qin

et al. 2020

Preprint

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Background: Due to the lack of specific and sensitive detection indicators, most patients with gastric cancer (GC) are already in the advanced stage at the time of diagnosis, resulting in a higher mortality. Therefore, it is urgent to search for effective diagnostic biomarkers with high specificity that can be applied in clinic. Methods: We screened out circ_0004771 through circRNA sequencing performed on three pairs of GC tissues and corresponding paracancerous tissues. Both exonuclease digestion assay, agarose gel electrophoresis (AGE) and sanger sequencing verified the potential of circ_0004771 being a biomarker. Additionally, we established quantitative real-time fluorescent Polymerase Chain Reaction (qRT-PCR) to detect the expression level of circ_0004771 and evaluated the methodology. What’s more, we collected plasma samples from GC patients, precancerous patients and gastritis patients, and we constructed the receiver operating characteristic curve (ROC) to appraise its diagnostic efficacy. Meanwhile, we collected the clinicopathological data of the patients to analyze the relationship between the expression level of circ_0004771 and the pathological parameters of the patients. Results: The expression level of circ_0004771 is up-regulated both in GC tissues and cells compared to normal controls. Circ_0004771 can be served as a promising biomarker because of its stable cyclic structure and longer half-life. The expression level of plasma circ_0004771 can distinguish between GC patients and precancerous patients, GC patients and gastritis patients. The diagnostic efficiency of circ_0004771 is higher than that of CEA and CA199. Higher diagnostic efficiency can be achieved in combination diagnosis. The expression level of plasma circ_0004771 in GC patients decreased after surgery, which can track the recovery condition of patients. Besides, the downstream regulatory forecast indicates that circ_0004771 may act as miRNA sponge to regulate the progression of GC.Conclusion: Plasma circ_0004771 can be served as a less invasive tumor biomarker with high diagnostic values.

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Clinical significance of hsa_circ_0000419 in gastric cancer screening and prognosis estimation

Cited by 37 publications

References 28 publications

CircHN1 affects cell proliferation and migration in gastric cancer

CircHN1 affects cell proliferation and migration in gastric cancer

<p>Comprehensive Assessment of Plasma Circ_0004771 as a Novel Diagnostic and Dynamic Monitoring Biomarker in Gastric Cancer</p>

Comprehensive Assessment of Plasma-Derived circ_0004771 as a Potential Biomarker in Tracking the Progression of Gastric Cancer

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