Clinical Significance of HMGB-1 and TGF-β Level in Serum and BALF of Advanced Non-Small Cell Lung Cancer

Abstract

Lung cancer is associated with poor prognosis. The aim of this study was to evaluate the clinical usefulness of HMGB-1 (high-mobility group protein B1) and TGF-β (transforming growth factor beta) in patients with advanced non-small cell lung cancer (NSCLC). We studied 45 patients with NSCLC prior to chemotherapy, 23 patients with Besnier-Boeck-Schaumann (BBS) disease (sarcoidosis), and 15 healthy volunteers. HMGB-1 and TGF-β levels were measured in serum and BALF samples using ELISA method. A higher serum HMGB-1 and TGF-β levels were in NSCLC patients compared with the other groups. TGF-β concentration in BALF was significantly higher in NSCLC than in healthy controls (p=0.047) but lower than in BBS (p=0.016). Serum HMGB-1 in NSCLC correlated with age and gender while its level in BALF was associated with distant metastasis. A higher levels of HMGB-1 in the serum of NSCLC patients with progressive disease was linked with shorter overall survival and disease-free survival. We found a positive correlation between HMGB-1 and TGF-β in BALF of IIIB NSCLC group and overall survival (p=0.04; p=0.003). Our findings confirmed that the measurement of HMGB-1 and TGF-β levels in serum and BALF of patients with NSCLC prior to treatment may have clinical usefulness and predict poor prognosis.

Soluble Toll-like receptor 4 is a potential serum biomarker in non-small cell lung cancer

“…Serum levels of HMGB1 of patients with NSCLC have also been demonstrated to be significantly higher than those in healthy controls [12, 13]. However, contradictory data on the biomarker function of HMGB1 in NSCLC have been reported [12, 13]. The results of our present work demonstrated an increasing trend of serum HMGB1 from stage I to stage III in patients with NSCLC without statistical significance.…”

“…HMGB1 expression is increased in the lungs of NSCLC patients and correlates with disease progression [29]. Serum levels of HMGB1 of patients with NSCLC have also been demonstrated to be significantly higher than those in healthy controls [12, 13]. However, contradictory data on the biomarker function of HMGB1 in NSCLC have been reported [12, 13].…”

Identification of lung cancer miRNA–miRNA co-regulation networks through a progressive data refining approach

“…TGF-beta signaling can inhibit tumor growth in earlystage tumors and contribute to lung cancer progression (Jeon and Jen, 2010;Jakubowska et al). All these existing studies provided a strong support for such a high hits in mTOR and TFG-beta signaling pathways in Fig.…”

The function and mechanism of HMGB1 in lung cancer and its potential therapeutic implications (Review)

“…In patients with NSCLC, the serum level of HMGB1 detected one month subsequent to surgery was significantly increased compared with the pre-operative level (43). An increased level of HMGB1 in the serum of patients with progressive NSCLC was associated with shorter overall survival and disease-free survival times (64). Therefore, HMGB1 may be considered as a potential biomarker for the diagnosis and prognosis of patients with NSCLC (45,46).…”