Clinical significance of elevated serum A-FABP and free fatty acid in neonates with hypoxic ischemic brain damage

Abstract

The main function of adipocyte fatty acid-binding protein (A-FABP) is to regulate fatty acid metabolism as its molecular chaperone. The clinical significance of A-FABP in hypoxic-ischemic brain damage (HIBD) neonates is not yet clear. Free fatty acid (FFA) in cerebral cortex increases along with hypoxia ischemia degree. Thus, we aimed to investigate whether FFA can induce A-FABP expression and elevate the serum A-FABP level in HIBD neonates. In the present study, 42 HIBD neonates were selected including 11 cases as mild, 16 cases as moderate and 15 cases as severe. The serum was collected from peripheral vein at 72 h after the first visit (acute stage) and 7 days after birth (recovery stage), and the serum from 10 normal neonates was used as the control. The serum level of A-FABP and FFA in 42 neonates with acute phase and recovery phase HIBD were detected using ELISA and copper colorimetric method. The overall serum A-FABP content in HIBD neonates at the acute stage was significantly higher compared to the normal neonates (P<0.05). The serum A-FABP level in severe HIBD neonates was significantly higher than that in mild HIBD, moderate HIBD and normal neonates (P<0.05). The serum FFA level in HIBD neonates at the acute stage was 1,521.57±605.63 µmol/l, which was significantly higher than that in the normal neonates 838.24±294.22 µmol/l. The serum FFA levels in mild, moderate and severe HIBD neonates were significantly higher than those in the normal neonates. The overall A-FABP level in HIBD neonates at the recovery stage was significantly lower compared to the acute stage, which was significant in severe HIBD neonates. A-FABP levels in mild and moderate HIBD neonates at recovery stage were decreased compared with the acute stage, although there was no statistical difference. There was a positive correlation between serum A-FABP and FFA in HIBD neonates at acute stage (r=0.369, P<0.05). In conclusion, serum A-FABP and FFA levels were signifcantly increased in HIBD neonates at acute stage, and were positively correlated. The serum A-FABP level in HIBD neonates at recovery stage was significantly lower than that in the acute stage. The results suggested that serum A-FABP and FFA levels at acute stage can reflect the severity of HIBD. The detection of serum A-FABP and FFA can be applied as indicators for the early diagnosis of HIBD, but also provides a basis for the clinical evaluation of HIBD treatment.

The Nutraceutical Value of Olive Oil and Its Bioactive Constituents on the Cardiovascular System. Focusing on Main Strategies to Slow Down Its Quality Decay during Production and Storage

“…Indeed, an increase thereof is associated with the pathogenesis of obesity and of obesity-related diseases [151,152]. Moreover, it has been found that there is a positive correlation between SFAs and the severity of hypoxic-damage in the brain, and finally, a direct proportionality emerged between the intake of SFAs and markers of acute myocardial infarct [153,154,155].…”

A comparative proteomic analysis for non‐invasive early prediction of hypoxic‐ischemic injury in asphyxiated neonates

“…Elevated FABP disrupts the blood-brain barrier and causes cerebral ischemic injury in mice [65]. Higher levels of FABP have been reported in the serum of neonates having a hypoxic injury [66].…”

“…Elevated FABP disrupts the blood‐brain barrier and causes cerebral ischemic injury in mice [65]. Higher levels of FABP have been reported in the serum of neonates having a hypoxic injury [66]. According to the Human Developmental Biology Resource (HDBR), the RNA‐seq data of prenatal human brain development shows FABP1 expression in the cerebellum, medulla oblongata, and spinal cord regions of neonatal brain [67].…”