Clinical significance of costimulatory molecules CD80/CD86 expression in IgA nephropathy

Wu, Qiong; Jinde, Kiichiro; Endoh, Masayuki; Sakai, Hideto

doi:10.1111/j.1523-1755.2004.00477.x

Cited by 37 publications

(23 citation statements)

References 30 publications

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“…These cells highly expressed CD73, CD90, and CD105, but lacked expression of CD14, CD34, and CD45 (Figure 3), indicating the absence of contaminating hematopoietic cells in the culture [22]. In accordance with previous reports, these cells also demonstrated a lack of the co-stimulatory molecules CD80 and CD86 [23], conferring the ability to escape from immune surveillance. The immunophenotypic characteristics of the cultured cells satisfied the criteria for MSC surface markers as defined by the International Society for Cellular Therapy [24].…”

Section: Discussionsupporting

confidence: 83%

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Mok

Leow

Koh

et al. 2017

IJMS

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Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.

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Section: Discussionsupporting

confidence: 83%

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Mok

Leow

Koh

et al. 2017

IJMS

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“…In IgAN, CD86 is predominantly expressed in the glomeruli and in the peritubular interstitium and CD80/CD86 expression levels correlate with renal function at the time of renal biopsy. Most CD86 + cells are monocytes and macrophages [20]. …”

Section: Introductionmentioning

confidence: 99%

CTLA-4 Polymorphisms in Patients with IgA Nephropathy Correlate with Proteinuria

Jacob

Ohl

Goodarzi

et al. 2018

Kidney Blood Press Res

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Background/Aims: IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis and still constitutes one of the most important causes of end-stage renal disease. Abnormal T cell responses may play a role in IgAN pathogenesis. Co-stimulatory molecules such as cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) are important for naive T cells to initiate and terminate immune responses. Single nucleotide polymorphisms (SNPs) in the CTLA4 gene locus are associated with several autoimmune diseases. Methods: We aimed to investigate the occurrence of the SNPs -318C/T, +49A/G and CT60 G/A within the CTLA4 locus in healthy blood donors (n=455) and IgAN patients (n=252) recruited from the recently published STOP-IgAN trial. The presence of these SNPs was then associated with baseline proteinuria in IgAN patients. Results: We observed a significantly increased frequency of the CTLA4 -318C/T genotype in IgAN patients as compared to controls (CC vs. CT+TT: OR 1.65, 95%-CI 1.03-2.65, p=0.035). No significant associations, neither with the +49A/G nor for the CT60 G/A SNP, were detected. However, when we stratified for proteinuria at time of inclusion into the STOP-IgAN trial (<1 g/day vs. >1 g/day), we observed significant differences in the frequencies of the CT60 G/A genotype, i.e. a significantly increased risk for higher proteinuria in patients carrying the G allele (OR 2.81, 95%-CI 1.03-7.64, p=0.042). Conclusion: The CTLA4 -318/C/T SNP was associated with an increased risk to develop IgAN, while the CT60 G/A genotype significantly associated with the risk for higher proteinuria suggesting a possible role for CTLA-4 in IgAN.

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“…In the present study, we have shown that renal biopsy of MpIgAN patients revealed increased interstitial cellular infiltration and tubular atrophy compared with SpIgAN patients. We found increased chemotaxis of CD45ϩ cells, CD3ϩ, CD4ϩ, CD8ϩ, CD20ϩ lymphocytes, and CD25ϩ monocytes in culture experiments in which tubular epithelial cells were stimulated with the IgA-HMC medium (21,22,44). In our in vitro culture setting, PBMC were chemoattracted to PTEC, which were exposed to the IgA-HMC medium on the apical surface.…”

Section: Discussionmentioning

confidence: 87%

In vitro enhanced chemotaxis of CD25+ mononuclear cells in patients with familial IgAN through glomerulotubular interactions

Tam

Leung

Chan

et al. 2010

American Journal of Physiology-Renal Physiology

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Tubulointerstitial infiltration of immunocompetent cells is often associated with a more rapid progression in IgA nephropathy (IgAN). Using an in vitro Transwell coculture system, we examined the chemotactic response of peripheral blood mononuclear cells to proximal tubular epithelial cells (PTEC) following activation by conditioned medium prepared from mesangial cells cultured with macromolecular IgA1 from 60 patients with multiplex familial IgAN (MpIgAN) and 91 of their asymptomatic relatives; 43 patients with sporadic IgAN (SpIgAN) and 90 of their asymptomatic relatives; and 43 healthy subjects. Compared with SpIgAN patients, PTEC activated by conditioned medium from patients with MpIgAN had elevated gene expression of a spectrum of C-C, C-X-C chemokines and proinflammatory cytokines, with prominent expressions of interleukin-6, interleukin-8, and tumor necrosis factor-α. In response to conditioned medium from patients with familial IgAN, there was a significant increase in chemotaxis of CD45+ cells, CD3+, CD4+, CD8+, CD20+ lymphocytes, and monocytes with CD25 expression. Our findings suggest that compared with SpIgAN patients, macromolecular IgA1 taken from MpIgAN patients is more pathogenic to cultured PTEC through a glomerulotubular interaction. A long-term follow-up is needed to better define the prognostic course for familial IgAN and to clarify the risk of developing IgAN in initially asymptomatic relatives from a multiplex IgAN family.

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Clinical significance of costimulatory molecules CD80/CD86 expression in IgA nephropathy

Abstract: This study suggested that CD80 and CD86 activate T cells in IgAN, CD80/CD86 expressions correlated with renal function at the time of renal biopsy, and monocyte/macrophages and tubular epithelial cells act as APC.

Cited by 37 publications

References 30 publications

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

CTLA-4 Polymorphisms in Patients with IgA Nephropathy Correlate with Proteinuria

In vitro enhanced chemotaxis of CD25+ mononuclear cells in patients with familial IgAN through glomerulotubular interactions

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