Clinical Significance of Beta-Thromboglobulin in Patients with High Platelet Count

Fabris, Fabrizio; Randi, M. L.; Casonato, Alessandra; Zanon, R. Dal Bo; Bonvicini, P.; Girolami, Antonio

doi:10.1159/000206503

Cited by 20 publications

(19 citation statements)

References 13 publications

(18 reference statements)

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“…Alterations in dense body content have been described in PT but not in ST. In particular, 5HT is considered a possible marker for PT (6). Currently, methods for measuring 5HT include radio enzymatic assay (7), radioimmunoassay (RIA) (8), '~uo~im~try (9), and high-performance liquid chromatography (HPLC) with electrochemical detection (10).…”

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confidence: 99%

Two Methods for Intraplatelet Serotonin Evaluation: Their Use in Thrombocytosis

Zerbinati

Randi

Rossi

et al. 1997

Clin Appl Thromb Hemost

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The content of platelet dense bodies is considered to be decreased in thrombocytosis associated myeloproliferative disorders and normal in reactive thrombocytosis. Intraplatelet serotonin i.e., 5-hydroxytryptamine (5HT), content is usually considered a particularly good marker. We report our experience of platelet 5HT levels evaluated by radioimmunoassay (RIA) and fluorimetric assays in patients with high platelet counts due to essential thrombocythemia (48 patients) or reactive thrombocytosis (34 patients). The diagnosis of essential thrombocythemia was made according to Polycythemia Vera Study Group criteria. A significant statistical difference was observed in serotonin platelet content between essential thrombocythemia and secondary thrombocytosis patients both with fluorimetric and RIA assays. On the contrary, no difference was evident between reactive thrombocytosis patients and normal control subjects.However, a significant difference was observed between 5HT in serum and platelet pellets when evaluated by RIA.Statistical analysis with the X 2 test showed that the sensitivity of the fluorimetric assay is slightly higher than that of the RIA assay; however, specificity is about the same with the two methods. It is possible that the two assays measure different molecules. At any rate, the fluorimetric assay seems to be better in evaluating the platelet content of 5HT at least in patients with high platelet counts. Key Words: Thrombocytosis—Serotonin.Serotonin 5-hydroxytryptamine, (5HT) is a biogenic amine found in many organs and tissues. Human platelets contain a high concentration of 5HT, stored in the dense bodies. 5HT is released together with ADP, catecholamines, calcium, and platelet factor 4 during the first phase of the release reaction (1,2). Serotonin is not synthesized by platelets, but is collected during their life spans (3): 5HT is carried through the platelet membrane to dense bodies where it complexes with adenine nucleotides (4). It is well known that primary thrombocytosis (PT) is associated with defective platelet function, while platelets in reactive or secondary thrombocytosis (ST) are usually functionary normal (5). Alterations in dense body content have been described in PT but not in ST. In particular, 5HT is considered a possible marker for PT (6). Currently, methods for measuring 5HT include radio enzymatic assay (7), radioimmunoassay (RIA) (8), '~uo~im~try (9), and high-performance liquid chromatography (HPLC) with electrochemical detection (10). We report here some observations about platelet 5HT content measured with radioimmunoassay (RIA) and fluorimetry in patients with high platelet count. MATERIAL AND METHODSEighty-two patients with platelet counts of >500 x 10~/L were included in this study. In 48 patients (13 men and 35 women, mean age, 55.25 ± 15.2 years), essential thrombocythemia (ET) was diagnosed in accordance with Polycythemia Vera Study Group Criteria (11,12). In 34 patients (11 men and 23 women, mean age, 46.5 -7.5 years), ST, resulting as reaction to neopl...

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Two Methods for Intraplatelet Serotonin Evaluation: Their Use in Thrombocytosis

Zerbinati

Randi

Rossi

et al. 1997

Clin Appl Thromb Hemost

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“…The results showed a lower incidence of thromboembolic acci dents in older ET patients as compared with the other groups and a lower percentage of hemorrhages in ET patients older than 70 years in comparison to younger ET subjects. However, platelet number and platelet function tests were similar in all the groups and did not differ from those of primary forms of thrombocy tosis [2,3,8,10].…”

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confidence: 85%

“…In agreement with this statement is also the observation that in 2 unrelated patients aged 65 and 64 years, re spectively, with residual thrombocytosis after treat ment of PV no thrombotic phenomena occurred over a 10-year observation period. From the laboratory data, no definitive predictive index on the incidence of thromboembolic complication can be obtained [2,3]. In fact, platelet serotonin was approximatly the same in every group studied.…”

Section: Discussionmentioning

confidence: 90%

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The Significance of Thrombocytosis in Old Age

Randi¹,

Casonato²,

Fabris³

et al. 1987

Acta Haematol

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We performed a retrospective study on 21 patients, aged 70 years or more, affected by poly-cythemia vera (PV) or essential thrombocythemia (ET). As controls, we evaluated 10 younger ET patients. The results indicate that in older ET subjects there was a lower incidence of hemorrhagic and thrombotic complications than in younger patients and PV patients. However, platelet number and platelet function tests were similar in all the patients studied. We suppose that an increase in hematocrit as seen in PV is much more dangerous as compared to an isolated increase in platelet count, and that thrombocytosis alone in old age can be an isolated expression of a natural involution of blood marrow similar to myelofibrosis

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“…The platelet aggregation was expressed as normal, increased or decreased in comparison to 100 controls comparable for sex and age. Platelet serotonin content (5-HT) was evaluated by a modification of the method proposed by Udenfriend as previously described [3] (normal value: 1.5-3 nAi/109 platelets).…”

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