Clinical Significance and Inflammatory Landscape of aNovel Recurrence-Associated Immune Signature in Stage II/III Colorectal Cancer

Abstract

BackgroundA considerable number of patients with stage II/III colorectal cancer (CRC) will relapse within 5 years after surgery, which is a leading cause of death in early-stage CRC. The current TNM stage system is limited due to the heterogeneous clinical outcomes displayed in patients of same stage. Therefore, searching for a novel tool to identify patients at high recurrence-risk for improving post-operative individual management is an urgent need.MethodsUsing four independent public cohorts and qRT-PCR data from 66 tissues, we developed and validated a recurrence-associated immune signature (RAIS) based on global immune genes. The clinical and molecular features, tumor immune microenvironment landscape, and immune checkpoints profiles of RAIS were also investigated.ResultsIn five independent cohorts, this novel scoring system was proven to be an independent recurrent factor and displayed excellent discrimination and calibration in predicting the recurrence-risk at 1~5 years. Further analysis revealed that the high-risk group displayed high mutation rate of TP53, while the low-risk group had more abundance of activated CD4+/CD8+ T cells and high expression of PD-1/PD-L1.ConclusionsThe RAIS model is highly predictive of recurrence in patients with stage II/III CRC, which might serve as a powerful tool to further optimize decision-making in adjuvant chemotherapy and immunotherapy, as well as tailor surveillance protocol for individual patients.

Prognostic Value of Tumor Mutational Burden Related to Immune Infiltration in Cervical Squamous Cell Carcinoma

“…The R package “maftools” was used to visualize mutation data. Moreover, the list of IRGs was obtained from the IMMPORT database ( https://www.immport.org/ ), which is extensively utilized in immune-related studies ( 42 , 43 ).…”

Computational Recognition and Clinical Verification of TGF-β-Derived miRNA Signature With Potential Implications in Prognosis and Immunotherapy of Intrahepatic Cholangiocarcinoma

“…We focused on their translational value in facilitating clinical management and found TGF-β-derived miRNAs demonstrated profound impacts on the prognosis of patients with ICC. In the present study, we were concerned that a single cohort with small size sample might identify many false-positive prognostic-related miRNAs, thus the screening criteria have been elevated as previous studies ( 35 , 36 ). In total, nine miRNAs that pronouncedly associated with OS and aberrantly expressed in ICC were further determined for signature discovery.…”

A Novel Lactate Metabolism-Related Gene Signature for Predicting Clinical Outcome and Tumor Microenvironment in Hepatocellular Carcinoma

“…The differentially expressed LMRGs were subjected to univariate Cox regression analysis to determine the LMRGs with prognostic value. To avoid overfitting, we further performed the LASSO Cox regression (iteration = 1000) using the “glmnet” package ( Friedman et al, 2010 ; Liu et al, 2021a ; Liu et al, 2021b ). After screening by LASSO regression, the selected LMRGs were applied to establish the LMRGS through the multivariate Cox regression analysis.…”