Clinical significance and impact of gastric non‐<i>Helicobacter pylori Helicobacter</i> species in gastric disease

Taillieu, Emily; Witte, Chloë De; Schepper, Heiko U. De; Moerkercke, Wouter Van; Rutten, Sophie; Michiels, Stijn; Arnst, Yuna; Bruyckere, Sofie De; Francque, Sven; Aert, Frauke van; George, Christophe; Callewaert, Emma; Callewaert, Tiene; Vanneste, Glenn; Vanderstraeten, Erik; Heddegem, Nina Van; Vansteelant, Margaux; Chiers, Koen; Haesebrouck, Freddy; Steenkiste, Christophe Van

doi:10.1111/apt.17488

Cited by 6 publications

(6 citation statements)

References 76 publications

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“…Moreover, some findings suggest a pathophysiological involvement of non- Hp Helicobacters in gastric diseases. 25 Therefore, more studies are needed to further explore these aspects in the future.…”

Section: Discussionmentioning

confidence: 99%

Eradicate Helicobacter pylori first, or treat peptic ulcer disease?

He,

Li,

2023

SAGE Open Medicine

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Background: Helicobacter pylori infection is very common worldwide, and about 10%–16% of these patients develop peptic ulcer disease. However, there is limited research on the impact of H. pylori eradication and peptic ulcer disease treatment sequencing. Methods: We conducted a retrospective study of adult patients diagnosed with H. pylori infection and peptic ulcer disease between October 2020 and April 2021 at our center. Data on primary treatment outcomes, including H. pylori eradication and peptic ulcer disease healing, were collected, and factors that may influence treatment outcomes were analyzed. Results: A total of 306 patients were included in this study. The sequence of H. pylori eradication and peptic ulcer disease treatment did not significantly affect the outcomes of H. pylori eradication and peptic ulcer disease healing. In addition, patient age, peptic ulcer disease type, clinic type and treatment regimen (including choice of proton pump inhibitor) had no significant impact on H. pylori eradication. However, patient gender and the choice of antibiotic combination proved to be key factors, as eradication rates were lower in female patients compared to males, and the combination of levofloxacin and clarithromycin was the least effective in eradicating H. pylori. Regarding peptic ulcer disease healing, the peptic ulcer disease type was an important influencing factor, since gastric ulcers being more likely to get cured completely compared to duodenal ulcers. Conclusions: The sequence of H. pylori eradication and peptic ulcer disease treatment does not significantly affect the primary outcomes. Patient gender and the choice of antibiotic combination are important factors in H. pylori eradication, whereas peptic ulcer disease type plays a key role in ulcer healing.

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Section: Discussionmentioning

confidence: 99%

Eradicate Helicobacter pylori first, or treat peptic ulcer disease?

He,

Li,

2023

SAGE Open Medicine

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“…A Helicobacter genus-specific PCR assay was performed as previously described [ 20 ]. Details on the primer sequences and thermocycling conditions can be found in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Two different Helicobacter -specific PCR assays were performed, one based on the ureAB gene and another based on the glmM gene [ 20 ]. Details on the primer sequences and thermocycling conditions can be found in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

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Presence of potentially novel Helicobacter pylori-like organisms in gastric samples from cats and dogs

Taillieu,

De Bruyckere,

Van Steenkiste

et al. 2023

Vet Res

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While seven gastric non-Helicobacter pylori Helicobacter (NHPH) species are known to commonly colonize the stomach of cats and dogs, the potential of H. pylori and H. pylori-like organisms to infect animals remains controversial and was investigated in this study using gastric samples of 20 cats and 27 dogs. A Helicobacter genus-specific 16 S rRNA PCR assay, H. pylori-specific ureAB and glmM PCR assays and a nested PCR detecting 23 S rRNA in a Helicobacter genus-specific manner in a first round of PCR and a H. pylori-specific manner in a second round, were performed in combination with sequencing. Histopathological and anti-Helicobacter immunohistochemical evaluations were also performed. Based on 16 S rRNA sequence analysis, 39/47 animals (83%) appeared infected with canine/feline gastric NHPHs in the corpus and/or antrum. H. pylori-specific ureAB amplicons were obtained in samples of 22 stomachs (47%). One canine antrum sample positive in the ureAB assay was also positive in the H. pylori-specific glmM assay. While 36/47 (77%) animals had a positive sample in the first round of the nested 23 S rRNA PCR assay, all samples were negative in the second round. Sequence analysis of obtained amplicons and immunohistochemistry point towards the presence of unidentified H. pylori-like organisms in cats and dogs. Histopathological examination suggests a low pathogenic significance of the gastric Helicobacter spp. present in these animals. In conclusion, cats and dogs may be (co-)infected with gastric Helicobacter organisms other than the known gastric NHPHs. Culture and isolation should be performed to confirm this hypothesis.

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“…Who doesn't like a good whodunit story? The study by Taillieu et al 1 is a ‘whodunit’ where, instead of a grisly murder, the crime scene is an inflamed stomach. The protagonists are inquisitive pathologists who, on the steps of their compatriot Hercule Poirot, scour gastric biopsies for an unseen offender.…”

mentioning

confidence: 99%