Clinical significance and expression of microRNA in diabetic patients with erectile dysfunction

Abstract

Abstract. The aim of the present study was to investigate the expression of microRNA (miR)-93, miR-320 and miR-16 and to assess their diagnostic value in diabetic patients with erectile dysfunction (ED). A total of 120 individuals were divided into three groups, which included the diabetics with ED group (ED group), the diabetics without ED group (NED group) and the healthy volunteers group (control group). Each group included 40 individuals. Serum samples were collected and reverse transcription quantitative polymerase chain reaction detection of the three types of miRNA was performed and the sensitivity of ED was analyzed by receiver operating characteristic curves. A negative correlation was identified between the incidence of ED in patients with diabetes and serum total testosterone levels (r=0.302, P<0.05); however, a positive correlation was observed between the incidence of ED in diabetics and the HbA1c level (r=0.231, P<0.05). Additionally, the relative expression levels of the three types of miRNA were higher in the ED group when compared with the NED and control groups (P<0.05). When compared with the control group, the area under the curve (AUC) values for miR-93, miR-320 and miR-16 were 0.793, 0.818 and 0.810, respectively, in the ED group and 0.576, 0.532 and 0.542 in the NED group, respectively. Furthermore, when compared with the NED group, the AUC value for miR-93, miR-320 and miR-16 was 0.707, 0.810 and 0.833, respectively, in the ED group. Therefore, the expression levels of miR-93, miR-320 and miR-16 may be useful for the early diagnosis of ED in patients with diabetes.

Decreased expression of microRNA‑30b promotes the development of pulpitis by upregulating the expression of interleukin‑6 receptor

“…MicroRNAs (miRNAs or miRs) are a type of non-coding, small RNA molecule made up of 18-22 nucleotides, which regulate the expression of proteins at mRNA level in eukaryotes (11)(12)(13). The pathogenesis of pulpitis is accompanied by changes in the expression of a variety of miRNA molecules and proteins, suggesting that miRNA may serve important roles in the regulation of proteins associated with the disease (14)(15)(16).…”

MicroRNA‑21 and its impact on signaling pathways in cervical cancer (Review)

“…MicroRNAs (miRs/miRNAs) are small non-coding RNAs that regulate protein at the mRNA level; they are 18 to 22 nucleotides in length, and are ubiquitous in eukaryotes (4)(5)(6). A number of studies have reported the abnormal expression of miRNAs in cervical cancer, including increased expression of miR-21 and miR-218, and reduced expression of miR-34 and miR-145 (7)(8)(9)(10).…”

MicroRNA-17 downregulates expression of the PTEN gene to promote the occurrence and development of adenomyosis

“…They are small RNA molecules of 18-22 nucleotides in length that specifically bind to the 3'-untranslated region (UTR) of mRNA to inhibit protein translation (9). Previous studies have determined that miRs serve important roles in tumors and various diseases, including cardiovascular disease and endocrine disease (10,11). Furthermore, miR-17 has been reported to serve important roles in the processes of tumor proliferation, invasion,…”