Clinical Significance and Applications of D-Ribose in Cardiovascular Disease

Bayram, Melike; David, P; St, Cyr Ja; T, Abraham William

doi:10.23937/iacvd-2017/1710013

Cited by 1 publication

(1 citation statement)

References 25 publications

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“…By elongating load duration or changing load type, it is expected that the engineered collagenous tissue properties as demonstrated in this study can be further manipulated 17,19,42,45,71 . To stretch the boundaries of our current approach, additives like TGF β 79 , ribose 80,81 , copper 82 or beta-aminopropionitrile 83 might be added, to further stimulate collagen deposition, induce AGE related crosslinking and enhance or inhibit LOX catalyzed crosslinking respectively. Lastly, plaque and cap rupture cannot be attributed to solely suboptimal collagenous properties.…”

Section: Discussionmentioning

confidence: 99%

Tissue-engineered collagenous fibrous cap models to systematically elucidate atherosclerotic plaque rupture

Wissing

Heiden

Serra

et al. 2021

Preprint

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A significant amount of vascular thrombotic events is associated with rupture of the fibrous cap that overlie atherosclerotic plaques. Cap rupture is however difficult to predict due to the heterogenous composition of the plaque, unknown material properties, and the stochastic nature of the event. Here, we aim to create tissue engineered human fibrous cap models with a variable but controllable collagen composition, suitable for mechanical testing, to scrutinize the reciprocal relationships between composition and mechanical properties. Myofibroblasts were cultured in 1 x 1.5 cm-sized fibrin-based constrained gels for 21 days according to established (dynamic) culture protocols (i.e. static, intermittent or continuous loading) to vary collagen composition (e.g. amount, type and organization). At day 7, a soft 2 mm diameter fibrin inclusion was introduced in the centre of each tissue to mimic the soft lipid core, simulating the heterogeneity of a plaque. Results demonstrate reproducible collagenous tissues, that mimic the bulk mechanical properties of human caps and vary in collagen composition due to the presence of an successfully integrated soft inclusion and the culture protocol applied. The models can be deployed to assess tissue mechanics, evolution and failure of fibrous caps or complex heterogeneous tissues in general.

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