Clinical roles and applications of progesterone in reproductive medicine: an overview

Filicori, Marco

doi:10.1111/aogs.12791

Cited by 17 publications

(10 citation statements)

References 29 publications

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“…The model targets used to validate this hypothesis were the steroids estradiol, progesterone, and testosterone. Steroids belong to a family of hormones involved in many physiological processes and are used as biomarkers in many fields, including reproductive health, 19,20 cancer diagnostics 21 as well as in doping control, 22 and the detection of environmental pollutants. 23,24 Steroid-binding aptamers have already been reported, with the majority of these reports targeting estradiol, and their binding properties were evaluated in a previous work.…”

Section: Introductionmentioning

confidence: 99%

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

et al. 2019

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Aptamers are well-established biorecognition molecules used in a wide variety of applications for the detection of their respective targets. However, individual SELEX processes typically performed for the identification of aptamers for each target can be quite time-consuming, labor-intensive, and costly. An alternative strategy is proposed herein for the simultaneous identification of different aptamers binding distinct but structurally similar targets in one single selection. This one-pot SELEX approach, using the steroids estradiol, progesterone, and testosterone as model targets, was achieved by combining the benefits of counter-SELEX with the power of next-generation sequencing and bioinformatics analysis. The pools from the last stage of the selection were compared in order to discover sequences with preferential abundance in only one of the pools. This led to the identification of aptamer candidates with potential specificity to a single steroid target. Binding studies demonstrated the high affinity of each selected aptamer for its respective target, and low nanomolar range dissociation constants calculated were similar to those previously reported for steroid-binding aptamers selected using traditional SELEX approaches. Finally, the selected aptamers were exploited in microtiter plate assays, achieving nanomolar limits of detection, while the specificity of these aptamers was also demonstrated. Overall, the one-pot SELEX strategy led to the discovery of aptamers for three different steroid targets in one single selection without compromising their affinity or specificity, demonstrating the power of this approach of aptamer discovery for the simultaneous selection of aptamers against multiple targets.

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Section: Introductionmentioning

confidence: 99%

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

et al. 2019

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“…The current study aims to certify the crucial roles of BMAL1 and other downstream factors in promoting trophoblast migration and invasion in RSA, and to validate the underlying molecular mechanisms. Additionally, numerous clinical studies suggest a positive action of progesterone in the maintenance of pregnancy and prevention of miscarriage [ 28 ]. Here, we try to characterize the effect of progesterone on the associated pathway.…”

Section: Introductionmentioning

confidence: 99%

BMAL1 facilitates trophoblast migration and invasion via SP1-DNMT1/DAB2IP pathway in recurrent spontaneous abortion

Zhai

Liu

et al. 2017

Oncotarget

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The underlying mechanism about rhythms and epigenetics leading to aberrant trophoblast migration and invasion in recurrent spontaneous abortion (RSA) remains unknown. Brain and muscle ARNT-like protein 1 (BMAL1) is considered as a crucial role in fertility, and polymorphism of BMAL1 gene has been reported to be associated with risk of miscarriage. However, the functional role of BMAL1 in RSA is not fully understood. Previous study shows the descended expression of DNA 5′-cytosine-methyltransferases 1 (DNMT1) in the villous of early pregnancy loss. Thus, understanding of the regulation of DNMT1 expression may be of significance for the elucidation of the process of RSA. Using HTR-8/SVneo and JEG-3 cell lines, we certified the induction of specificity protein 1 (SP1) to DNMT1 and DAB2 interaction protein (DAB2IP), respectively, both of which further activated matrix metallo-proteinase 2/9 (MMP2/9), bringing out changes in trophoblast migration and invasion. Notably, BMAL1 functioned as a positive upstream factor of SP1 only in HTR-8/SVneo cells but not in JEG-3 cells, inducing SP1-DNMT1/DAB2IP pathway and facilitating migration and invasion of trophoblasts. In addition, progesterone might restore the down-regulation of BMAL1 and downstream pathway in a dose-dependent manner. Last but not least, the decreased abundance of BMAL1 was correlated positively with that of SP1, DNMT1, DAB2IP, MMP2 and MMP9 in human villous specimens of RSA. Our results demonstrate that the induction of BMAL1 to SP1 contributes to the expression of DNMT1 and DAB2IP, respectively, activating trophoblast migration and invasion. The deregulation of the BMAL1-mediated pathway in RSA can be rescued by progesterone.

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“…Women stimulated with clomiphene citrate to induce ovulation do not seem to benefit from this treatment. Progesterone administration can be initiated at the time of oocyte retrieval, or 1-2 days before embryo transfer, and continued until the first positive hCG test or until the onset of menstruation [75,76].…”

Section: Progesterone Treatment In Assisted Reproductionmentioning

confidence: 99%

“…Although progesterone supplementation beyond the first positive pregnancy test after IVF/ICSI might generally be unnecessary [77], most IVF centers have elected to continue progesterone administration until the 6th-10th week of pregnancy to minimize the risk of miscarriage [75].…”

Section: Progesterone Treatment In Assisted Reproductionmentioning

confidence: 99%

“…Progesterone administration maintains uterine quiescence in late pregnancy and delays delivery, particularly in patients with short uterine cervixes [75]. Recent metaanalysis proved that vaginal progesterone decreases the risk of preterm birth and improves perinatal outcomes in singleton gestations with a mid-trimester sonographic cervical length ≤25 mm [79].…”

Section: Maintenance Of Uterine Quiescence In Late Pregnancymentioning

confidence: 99%

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Key to Life: Physiological Role and Clinical Implications of Progesterone

Nagy

Szekeres-Barthó

Kovaćs

et al. 2021

IJMS

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The most recent studies of progesterone research provide remarkable insights into the physiological role and clinical importance of this hormone. Although the name progesterone itself means “promoting gestation”, this steroid hormone is far more than a gestational agent. Progesterone is recognized as a key physiological component of not only the menstrual cycle and pregnancy but also as an essential steroidogenic precursor of other gonadal and non-gonadal hormones such as aldosterone, cortisol, estradiol, and testosterone. Based on current findings, progesterone and novel progesterone-based drugs have many important functions, including contraception, treatment of dysfunctional uterine bleeding, immune response, and prevention of cancer. Considering the above, reproduction and life are not possible without progesterone; thus, a better understanding of this essential molecule could enable safe and effective use of this hormone in many clinical conditions.

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Clinical roles and applications of progesterone in reproductive medicine: an overview

Cited by 17 publications

References 29 publications

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

BMAL1 facilitates trophoblast migration and invasion via SP1-DNMT1/DAB2IP pathway in recurrent spontaneous abortion

Key to Life: Physiological Role and Clinical Implications of Progesterone

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