Clinical Response to Hormone Therapy Correlated With Estrogen Receptor Analyses: Biochemical v Histo-chemical Methods

McCarty, Kenneth S.; Hiatt, K.B.; Budwit, Debra; Cox, Edwin B.; Leight, C.; Reintgen, Douglas S.; Ceorgiade, C.; Siegler, Hilliard F.

doi:10.1016/s0022-5347(17)50760-0

Cited by 4 publications

(4 citation statements)

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“…Immunohistochemical assay of hormone receptors has gained acceptance as a less time‐consuming and less expensive method compared to the biological method mentioned earlier 2,23 . This assay also provides more accurate results (as it is possible to confirm the presence of tumor heterogeneity) of non‐immunoreactive stromal elements and of immunoreactive normal breast elements, all of which would have inaccurate results with a biochemical assay 24 .…”

Section: Discussionmentioning

confidence: 99%

Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas

Hatanaka¹,

Hashizume²,

Nitta³

et al. 2003

Pathology International

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A cytometrical image analyzing method for nuclear protein was established using WinROOF, a commercially available, inexpensive software, to determine the status of both estrogen and progesterone receptors. Immunohistochemical evaluation of estrogen receptors (ER) and progesterone receptors (PR) was performed with the anti-ER (clone 1D5) and the anti-PR (clone PgR636), respectively, combined with dextran polymer reagent EnVision+, all of which are approved in vitro diagnostics in Japan. The immunostained results were captured as digital images in Windows, and then analyzed in WinROOF with macroinstructions for analyzing each captured area either immunolabeled with chromogen or counterstained with hematoxylin. This image analysis method graded the immunostained nuclei of carcinoma cells based on staining intensities, and calculated the labeling index (LI) for both ER and PR. Furthermore, the LI correlated highly with the results from a histology score (HSCORE) when 20 breast carcinomas were quantified. Regarding ER, when 20% in the LI was considered as the cut-off point for positive, the positivity of ER in computer-assisted analysis was 75% (15 of 20 cases), and was completely concordant with that of HSCORE-based analysis. These results indicate that the cytometrical image analysis-based quantification could be appropriately applied to the objective determination of the immunohistochemical status of both ER and PR.

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Section: Discussionmentioning

confidence: 99%

Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas

Hatanaka¹,

Hashizume²,

Nitta³

et al. 2003

Pathology International

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“…Reasons for discordance are not obvious but may relate to the ligand employed, criteria for positivity, and degree of expertise in both histopathology and fluorescence microscopy. There is preliminary evidence that histochemical estrogen-binding assays are of clinical value in the prediction of response to endocrine therapies in advanced breast cancer [4245], although this is controversial [46].…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of Steroid Binding Sites in Prostatic Carcinoma: Morphological Demonstration and Clinical Implications

et al. 1985

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Prostatic neoplasms were studied for estrogen binding using four methods. Two employed fluorescent estrogen histochemical ligands, one was a new immunocytochemical technique using specific monoclonal antibodies to human estrophilin, and the last procedure was conventional biochemical dextran-coated charcoal assay. Results indicated that the fluorescent ligands recognized closely associated but separate estrogen-binding sites (putative type II sites) which in turn differed from the binding site measured biochemically. Studies with the monoclonal antibodies were nearly always negative, suggesting that prostatic estrogen receptor might vary antigenically from that present in breast and endometrium. Histochemical and biochemical androgen-binding studies were also compared and showed a close association. In the prediction of hormonal response in advanced prostate cancer both showed high sensitivity and low specificity. The addition of estrogen-binding data did not improve the predictive value of the androgen-binding histochemical assay. However, combining results of the biochemical and histochemical androgen-binding assays resulted in significant improvement of the specificity without loss of sensitivity, suggesting that there is a degree of positive interaction between the binding sites assayed by the two methods.

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“…Each specimen was scored semiquantitatively using the H-score system [10], with the H-score being a product of the intensity of nuclear staining (range 0-3, with 0 indicating no staining and 3 indicating strong staining) and the percentage of tumor cells stained at each intensity across cellular tissue segments on each slide as follows: H-Score = (percentage of cells stained at intensity category 0) × 0 + (percentage of cells stained at intensity category 1) × 1 + (percentage of cells stained at intensity category at 2) × 2 + (percentage of cells stained at intensity category 3) × 3.…”

Section: Methodsmentioning

confidence: 99%

Clinical correlates of leiomyoma estrogen and progesterone receptors among Nigerian women

Awowole

Makinde

Badejoko

et al. 2016

Intl J Gynecology & Obste

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Clinical Response to Hormone Therapy Correlated With Estrogen Receptor Analyses: Biochemical v Histo-chemical Methods

Cited by 4 publications

References 0 publications

Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas

Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas

Heterogeneity of Steroid Binding Sites in Prostatic Carcinoma: Morphological Demonstration and Clinical Implications

Clinical correlates of leiomyoma estrogen and progesterone receptors among Nigerian women

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