Clinical Research Assessment By Flow Cytometry of Biomarkers for Infectious Stratification in An Emergency Department

Bourgoin, P; Soliveres, Thomas; Ahriz, Dalia; Arnoux, Isabelle; Meisel, Christian; Unterwalder, Nadine; Morange, Pierre‐Emmanuel; Michelet, Pierre; Malergue, Fabrice; Markarian, Thibaut

doi:10.2217/bmm-2019-0214

Cited by 20 publications

(15 citation statements)

References 44 publications

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“…Alternatively, overexpression of mCD169 (siglec‐1, one of ISG) by whole blood flow cytometry has been recently proposed as a sensitive and specific surrogate marker of viral infection within the context of early COVID‐19 6 . This marker was previously described as a tool, in conjunction with nCD64 expression, to discriminate bacterial from viral infections at emergency room 5,15 . Specific kinetics of mCD169 expression in response to IFN‐I has been described in vitro 4 …”

Section: Discussionmentioning

confidence: 99%

“…Therefore, increased mCD169 has been proposed, by itself, as a potential host marker of viral infections (e.g., HIV, influenza). Moreover, in conjunction with concomitant assessment of neutrophil CD64 (nCD64) expression, mCD169 was used to differentiate viral from bacterial infections 5 . Recently, along with increased plasma IFN‐I concentration, mCD169 overexpression was observed in COVID‐19 patients 6 .…”

Section: Introductionmentioning

confidence: 99%

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Monocyte CD169 expression in COVID‐19 patients upon intensive care unit admission

et al. 2021

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During the second surge of COVID‐19 in France (fall 2020), we assessed the expression of monocyte CD169 (i.e., Siglec‐1, one of the numerous IFN‐stimulated genes) upon admission to intensive care units of 45 patients with RT‐PCR‐confirmed SARS‐CoV2 pulmonary infection. Overall, CD169 expression was strongly induced on circulating monocytes of COVID‐19 patients compared with healthy donors and patients with bacterial sepsis. Beyond its contribution at the emergency department, CD169 testing may be also helpful for patients' triage at the ICU to rapidly reinforce suspicion of COVID‐19 etiology in patients with acute respiratory failure awaiting for PCR results for definitive diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monocyte CD169 expression in COVID‐19 patients upon intensive care unit admission

et al. 2021

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“…Thus, the combinatorial detection of CD64 and CD169, respectively, on the surface of neutrophils and monocytes, could be a specific measure for the distinction between the different causes of infections 13 . The main goal of the study was to understand the link that exists between both biomarkers and IFNs produced in response to infections.…”

Section: Introductionmentioning

confidence: 99%

“…11,12 Thus, the combinatorial detection of CD64 and CD169, respectively, on the surface of neutrophils and monocytes, could be a specific measure for the distinction between the different causes of infections. 13 The main goal of the study was to understand the link that exists between both biomarkers and IFNs produced in response to infections. The main hypothesis was that the understanding of this functional link could help demonstrate the relevance of using these biomarkers for discriminating between bacterial and viral infections.…”

mentioning

confidence: 99%

Role of the interferons in CD64 and CD169 expressions in whole blood: Relevance in the balance between viral‐ or bacterial‐oriented immune responses

Bourgoin

Biéchelé

Belkacem

et al. 2020

Immunity Inflam & Disease

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Introduction: CD64 expression increases on neutrophils during bacterial infections. Recently an increase in CD169 expression has been discovered on monocytes during viral infections. Generally, interferons α (IFNsα) andIFNsγ are key drivers of the infectious host immune response. The purpose of this study was to explore if a link exists between these IFNs and both biomarkers. Methods: Whole blood samples from healthy volunteers were stimulated with either IFNs, interleukins, or infectious extracts, to mimic an infectious state. Expressions of CD64 and CD169 were assessed in these samples by multiple flow cytometry methods, over precise kinetics.Results: The expression of CD64 was statistically higher in samples stimulated with IFNγ, and CD169 in those stimulated with IFNα (and all other type I IFNs).Surface expressions are directly induced by their respective IFNs via Janus kinase/ signal transducer and activator of transduction pathways within 6 to 8 hours of incubation. Mixing both types of IFNs seemed to indicate that they partially inhibit each other. Conclusions: The induction of CD169 on monocytes and CD164 on neutrophils by type I and type II IFNs confirms the relevance of these markers for assessing between a viral-vs bacterial-oriented immune response.

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“…Indeed, leukocytes detect and rapidly respond to infection with secreted and surface activation molecules. We and others have previously reported that acute viral infections induce the appearance of CD169 (Siglec-1, sialoadhesin) at the surface of blood monocytes (6,7). Monocyte CD169 expression is upregulated by type 1 interferons (8), produced by locally attacked tissues, and is found in all circulating blood monocytes, allowing its detection in minimal blood volumes such as a drop of blood at the fingertip.…”

Section: Introductionmentioning

confidence: 99%

An ultra-sensitive, ultra-fast whole blood monocyte CD169 assay for COVID-19 screening

Michel

Malergue²,

Belkacem³

et al. 2020

Preprint

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CoVID-19 is an unprecedented epidemic, globally challenging health systems, societies, and economy. Its diagnosis relies on molecular methods, with drawbacks revealed by current use as mass screening. Monocyte CD169 upregulation has been reported as a marker of viral infections, we evaluated a flow cytometry three-color rapid assay of whole blood monocyte CD169 for CoVID-19 screening. Outpatients (n=177) with confirmed CoVID-19 infection, comprising 80 early-stage (≤14 days after symptom onset), 71 late-stage (≥15 days), and 26 asymptomatic patients received whole blood CD169 testing in parallel with SARS-CoV-2 RT-PCR. Upregulation of monocyte CD169 without polymorphonuclear neutrophil CD64 changes was the primary endpoint. Sensitivity was 98% and 100% in early-stage and asymptomatic patients respectively, specificity was 50% and 84%. Rapid whole blood monocyte CD169 evaluation was highly sensitive when compared with RT-PCR, especially in early-stage, asymptomatic patients whose RT-PCR tests were not yet positive. Diagnostic accuracy, easy finger prick sampling and minimal time-to-result (15-30 minutes) rank whole blood monocyte CD169 upregulation as a potential screening and diagnostic support for CoVID-19. Secondary endpoints were neutrophil CD64 upregulation as a marker of bacterial infections and monocyte HLA-DR downregulation as a surrogate of immune fitness, both assisting with adequate and rapid management of non-CoVID cases.

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Clinical Research Assessment By Flow Cytometry of Biomarkers for Infectious Stratification in An Emergency Department

Cited by 20 publications

References 44 publications

Monocyte CD169 expression in COVID‐19 patients upon intensive care unit admission

Monocyte CD169 expression in COVID‐19 patients upon intensive care unit admission

Role of the interferons in CD64 and CD169 expressions in whole blood: Relevance in the balance between viral‐ or bacterial‐oriented immune responses

An ultra-sensitive, ultra-fast whole blood monocyte CD169 assay for COVID-19 screening

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