Clinical relevance of the cagA and vacA s1m1 status and antibiotic resistance in Helicobacter pylori: a systematic review and meta-analysis

Karbalaei, Mohsen; Abadi, Amin Talebi Bezmin; Keikha, Masoud

doi:10.1186/s12879-022-07546-5

Cited by 20 publications

(10 citation statements)

References 91 publications

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“…Our results showed that 72.7% of gastric adenocarcinoma samples were negative for p27 expression, which means that the lack of p27 expression can be an essential change during gastric carcinogenesis. More recent research indicates differential resistance of anti-microbial therapeutics worthy of further investigation between cagA positive and vacA positive H. pylori [62].…”

Section: Discussionmentioning

confidence: 99%

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

Al-Sheboul

Mohammad

Shboul

et al. 2023

J Epidemiol Glob Health

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Stomach (gastric) cancer is one of the most prevalent and deadly cancers worldwide and most gastric cancers are adenocarcinomas. Based on prior research, there is an association between Helicobacter pylori (H. pylori) infection together with the frequency of duodenal ulcer, distal gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and antral gastritis. Helicobacter pylori virulence and toxicity factors have been identified before that significantly influence the clinical outcomes of H. pylori infection and gastric adenocarcinoma. However, it remains unclear exactly how different strains of H. pylori affect gastric adenocarcinoma. Current research suggests this involves tumor suppressor genes, like p27 but also H. pylori toxic virulence proteins. Therefore, we quantified known H. pylori genotypes within adenocarcinoma patients to establish the prevalence of known toxins that include cytotoxin-associated gene A (cagA) as well as vacuolating cytotoxin A (vacA) within patients of variable adenocarcinoma diagnosis. This analysis used gastrectomy samples validated for DNA viability. The incidence of H. pylori in adenocarcinoma patients in Jordan was established to be 54.5% positive (ureA gene positive) with cagA genotype occurrence at 57.1%, but also in this population study vacA gene ratios found to be 24.7%:22.1%:14.3%:14.3%. (vacAs1:vacAs2:vacAm1:vacAm2). Using immunohistochemistry (IHC), we confirmed with statistical significance that p27 was dysregulated and suppressed, within nearly all H. pylori vacA genotypes. In addition, within 24.6% of H. pylori samples analyzed was a different bacterial genotype, and curiously that p27 protein expression was retained in 12% of tested adenocarcinoma H. pylori samples. This is suggestive that p27 could be used as a prognostic indicator but also that an unknown genotype could be contributing to the regulatory effects of p27 protein within this bacterial and cellular environment that may include other virulence factors and unknown immune system regulatory changes.

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Section: Discussionmentioning

confidence: 99%

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

Al-Sheboul

Mohammad

Shboul

et al. 2023

J Epidemiol Glob Health

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Section: Discussionmentioning

confidence: 99%

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

Brown

Mohammad

Shboul

et al. 2022

Preprint

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Gastric cancer is one of the most prevalent malignant cancers worldwide and specifically, adenocarcinoma. Based on prior research, there is an association between Helicobacter pylori (H. pylori) infection and the frequency of duodenal ulcer, distal gastric adenocarcinoma, mucosa-associated lymphoid tissue lymphoma, and antral gastritis. H. pylori virulence and toxicity factors have been identified that significantly influence the clinical outcomes of H. pylori infection and gastric adenocarcinoma. However, it is unclear exactly how different strains of H. pylori infection affect gastric adenocarcinoma. Current research suggests this involves tumor suppressor genes, like p27, but also H. Pylori toxic proteins. Therefore, we quantified known H. Pylori genotypes within adenocarcinoma patients to establish the prevalence of known toxins that include cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) within patients of variable diagnosis. This analysis used gastrectomy samples which were validated for DNA viability. The incidence of H. Pylori in adenocarcinoma patients in Jordan was established to be 54.5% positive (ureA gene) with cagA genotype occurrence at 57.1% but also vacA gene ratios 24.7%:22.1%: 14.3%:14.3%. (vacAs1:vacAs2: vacAm1:vacAm2). It is statistically significant that p27 was dysregulated and suppressed within nearly all H. Pylori vacA genotypes but also that according to our analysis that 24.6% of H. Pylori samples analyzed had an unknown novel genotype and curiously that p27 protein expression was retained in only 12% of tested adenocarcinoma H. Pylori samples which is suggestive that p27 could be used as a prognostic indicator but also that an un-known yet genotype as yet not characterized could be contributing to the regulation of p27 protein within this cellular environment.

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“…Провідне місце в лікуванні виразкової хвороби відводиться антибактеріальному впливу на мікроорганізм H.pylori. Антибактеріальна терапія -найбільш жорстко регламентований у всьому світі розділ фармакотерапії [6]. Це продиктовано міркуваннями ефективності та безпеки використання медичної технології.…”

Section: резюмеunclassified

Effectiveness of the modified scheme for treatment of Н.pylori-associated peptic ulcer of the duodenum in children

Sorokman

Moldovan

2022

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Background. None of the known regimens for the treatment of H.pylori-associated peptic ulcer of the duodenum (PUD) is 100 % effective. The purpose was to evaluate the effectiveness of the modified comprehensive therapy for Н.pylori-associated peptic ulcer of the duodenum in children. Materials and methods. The study included 66 patients aged 7 to 18 years with Н.pylori-associated PUD in the acute stage. Serum vitamin D levels were measured using the electrochemiluminescence method (Roche Diagnostics GmbH, Mannheim, Germany). The classical method of stool culture was used. The children were divided into three groups: the first — 20 patients who received protocol eradication therapy, the second — 23 children who received modified comprehensive therapy with the inclusion of vitamin D3, the third — 23 patients who received modified comprehensive therapy with the inclusion of vitamin D3 and a synbiotic. Results. The average length of stay in the hospital for patients who received modified therapy with the inclusion of vitamin D3 was reduced by 18.8 %, and in those who received synbiotic additionally — by 23.8 % compared to the first group. During the endoscopic examination conducted after 1 month, the presence of an elongated red scar at the site of the ulcer defect was observed significantly more often in group I (65.5 %) than in groups II (47.8 %) and III (39.1 %), χ2 = 3.87, p < 0.05; after 1 year, 33.3 % of children in group I, 17.3 % in group II, and 8.7 % in group III (p < 0.01) had a recurrence of the disease. The determination of the main indicators for the effectiveness of PUD treatment using a modified scheme showed that the relative risk of disease recurrence will decrease by 0.42 times (χ2 = 3.96, p < 0.05) when vitamin D3 preparation is included in the treatment scheme and by 0.52 times (χ2 = 4.06, p < 0.01) when using vitamin D3 and a synbiotic. Conclusions. 1. Children with Н.pylori-associated peptic ulcer of the duodenum have vitamin D3 deficiency and various disturbances of the intestinal microbiota, which can complicate the course, increase the chronicity of the process, and reduce the effectiveness of anti-Helicobacter therapy. 2. The use of a modified comprehensive treatment for duodenal ulcer associated with H.pylori leads to a reduction in the duration of the main clinical manifestations of the disease, the duration of inpatient treatment of sick children and a decrease in the frequency of recurrence of the disease.

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Clinical relevance of the cagA and vacA s1m1 status and antibiotic resistance in Helicobacter pylori: a systematic review and meta-analysis

Cited by 20 publications

References 91 publications

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

Effectiveness of the modified scheme for treatment of Н.pylori-associated peptic ulcer of the duodenum in children

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