Clinical relevance of cagA and vacA gene polymorphisms in Helicobacter pylori isolates from Senegalese patients

Breurec, Sébastien; Michel, R.; Seck, Abdoulaye; Brisse, Sylvain; Côme, D.; Dieye, Fatou Bintou; Garin, Benoît; Huerre, M; Mbengue, M.; Fall, Cheikh; Sgouras, Dionyssios N.; Thiberge, Jean‐Michel; Dia, D.; Raymond, Josette

doi:10.1111/j.1469-0691.2011.03524.x

Cited by 29 publications

(35 citation statements)

References 28 publications

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“…In one study the prevalence of cagA gene was 48.7%, lower than other reports in African countries (17) and was present in 73.3% of isolates (6) . In patient positives for H. pylori in gastric biopsies, the cagA gene was detected in 43.3% of gastric biopsies, in 43.8% of saliva samples, and in 27.3% of dental plaque samples, noting that dental plaque and saliva can serve as temporary storage for the cagA variant H. pylori in individuals with gastric disease (34) .…”

Section: Discussioncontrasting

confidence: 54%

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Medina¹,

Medina

Merino

2017

Arq. Gastroenterol.

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-Background -The clinical outcome of Helicobacter pylori infection has been associated with virulence factors. The presence of these factors is useful as molecular markers in the identification of the high risk for developing severe gastric pathologies. Objective -To correlate the presence of virulence markers cagA and bab2A of H. pylori in oral and gastric biopsy samples. Methods -An observational, prospective, descriptive, and cross-sectional study was carried out between September 2011 and September 2012. Patients suffering dyspepsia with indication for upper gastrointestinal video endoscopy who attended the Gastroenterology Service of the Hospital Dr. Julio C. Perrando were included. Epidemiological investigation was completed. To detect the bacteria and their virulence genes, samples of saliva, dental plaque and gastric biopsy were taken and processed by PCR.Results -Sixty-one patients were selected for this study (30 women and 31 men). H. pylori was detected in 31 gastric biopsies and 31 oral samples.Significant difference between oral and gastric samples was found in cagA genotype. Agreement between oral and gastric genotypes was found in 38.7% of samples from the same patient. Conclusion -This study is the first in provide information about the genotypes of the Argentinean Northeast H. pylori strains. Despite the high prevalence of H. pylori infection, the most of patients had less virulent genotypes in oral cavity and gastric tissue. The cagA/babA2 combination was not frequent in the samples studied. There was not a statistical correlation between the virulence genes and gastroduodenal or oral diseases. Although in some patients the same genotype was found both in oral and gastric samples, it cannot be ensure that they corresponding to the same strain because a DNA sequencing was not performed.

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Section: Discussioncontrasting

confidence: 54%

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Medina¹,

Medina

Merino

2017

Arq. Gastroenterol.

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“…Recent data also demonstrates the influence of Helicobacter pylori virulence factors on clinical and endoscopic disease in an endemic region like sub-Saharan Africa [10]. Here, for the first time we present data on characterization of H. pylori cagA and vacA genotypes and their association with gastro-duodenal disease in Ghanaian patients.…”

Section: Introductionmentioning

confidence: 77%

Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients

et al. 2017

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Background Helicobacter pylori infection is prevalent in Ghana. The development of gastro-duodenal disease is dependent on virulence of the infecting strain, host susceptibility and environmental factors. Helicobacter pylori cagA and vacA strains induce more inflammation, ulceration and oncogenesis. Here, for the first time we present data on H. pylori cagA and vacA genes and their association with gastro-duodenal disease in Ghana. A total of 159 patients with dyspepsia at Korle-Bu Teaching Hospital, Accra, were investigated for H. pylori with urease-CLO, of which 113 (71.1%) were positive. Genomic DNA was extracted from antral biopsies using QIAGEN DNeasy kit. Detection of H. pylori vacA and cagA genes were determined by PCR as previously described.ResultsIn total, 110 (69.2%) vacAs1, 71 (44.7%) vacAm1, 35 (22.0%) vacAm2, 77 (48.4%) cagA-(hydrophilic region) and 109 (68.6%) cagA-(internal duplication region) were detected. In multivariate analysis, duodenal ulcer was more likely than other diagnoses to have detectable cagA-(hydrophilic region) (OR 3.1 CI 1.2–7.9) or vacAs1m1 (OR 6.5 CI 1.2–34.0).ConclusionsMajority of biopsies were colonized with H. pylori harboring both cagA and vacA. H. pylori cagA-(internal duplication region) was more prevalent than cagA-(hydrophilic region). Duodenal ulcer was more likely than other diagnoses to have detectable cagA-(hydrophilic region) or vacAs1m1.

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“…H. pylori was cultured and identified as previously described [19]. A single H. pylori colony from the antrum or fundus was chosen and subcultured from a primary growth.…”

Section: Methodsmentioning

confidence: 99%

Population Genetic Structure and Isolation by Distance of Helicobacter pylori in Senegal and Madagascar

et al. 2014

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Helicobacter pylori has probably infected the human stomach since our origins and subsequently diversified in parallel with their human hosts. The genetic population history of H. pylori can therefore be used as a marker for human migration. We analysed seven housekeeping gene sequences of H. pylori strains isolated from 78 Senegalese and 24 Malagasy patients and compared them with the sequences of strains from other geographical locations. H. pylori from Senegal and Madagascar can be placed in the previously described HpAfrica1 genetic population, subpopulations hspWAfrica and hspSAfrica, respectively. These 2 subpopulations correspond to the distribution of Niger-Congo speakers in West and most of subequatorial Africa (due to Bantu migrations), respectively. H. pylori appears as a single population in Senegal, indicating a long common history between ethnicities as well as frequent local admixtures. The lack of differentiation between these isolates and an increasing genetic differentiation with geographical distance between sampling locations in Africa was evidence for genetic isolation by distance. The Austronesian expansion that started from Taiwan 5000 years ago dispersed one of the 10 subgroups of the Austronesian language family via insular Southeast Asia into the Pacific and Madagascar, and hspMaori is a marker for the entire Austronesian expansion. Strain competition and replacement of hspMaori by hpAfrica1 strains from Bantu migrants are the probable reasons for the presence of hspSAfrica strains in Malagasy of Southeast Asian descent. hpAfrica1 strains appear to be generalist strains that have the necessary genetic diversity to efficiently colonise a wide host spectrum.

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Clinical relevance of cagA and vacA gene polymorphisms in Helicobacter pylori isolates from Senegalese patients

Cited by 29 publications

References 28 publications

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients

Population Genetic Structure and Isolation by Distance of Helicobacter pylori in Senegal and Madagascar

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