Clinical Relevance of a Serological Cross-Reaction Between Escherichia Coli 0157 and Brucella Abortus

Notenboom, Robert; Borczyk, A; Karmali, Mohamed A.; Duncan, Linda

doi:10.1016/s0140-6736(87)91106-8

Cited by 21 publications

(23 citation statements)

References 4 publications

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“…Detection of serum agglutinating antibodies was used with some success by Notenboom et al 17 In our study, 17 (60.7%) of 28 cases had titres of agglutinating antibody 2 160, whereas this was true of only nine (26.4 %) of 34 control patients ; this simple, rapid and economical test may be a useful adjunct to laboratory diagnosis.…”

Section: Discussionmentioning

confidence: 53%

Detection of serum and faecal antibodies in haemorrhagic colitis caused by Escherichia coli O157

Siddons¹,

Chapman²

1993

Journal of Medical Microbiology

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Summary. Cases of culture-confirmed clinically typical haemorrhagic colitis caused by verocytotoxin-producing (VT+) Escherichia coli 0 157 and age-and sex-matched control patients were examined for antibodies to E. coli 0157. Serum samples from 28 cases and 34 patients in control group 1 were examined for VT,-and VT,-neutralising antibodies, E. coli 0 157 agglutinating antibodies, and by an enzyme immunoassay (EIA) technique for IgG antibodies against smooth lipopolysaccharide purified from E. coli 0157 and for IgG antibodies against whole intact E. coli 0 1 57 cells. Differences between antibody titres were significant when compared by a Wilcoxon two-sample test for E. coli 0157 agglutinating antibodies (p < 0.05) and IgG antibodies against whole cells (p < 0.001). The whole-cell EIA was used further to examine faecal samples from 93 cases and 47 patients in control group 2 for IgA antibodies. Elevated levels of faecal IgA specific for E. coli 0157 were found in 59 (63.4 O/ O) of 93 cases but in only 10 (21.2 YO) of 47 control patients (p < 0.001); follow-up faecal samples from five cases all showed marked rises in levels of IgA that appeared to coincide with cessation of excretion of the organism. Detection of specific faecal IgA with a whole-cell EIA, although requiring further evaluation, may be a useful addition to tests currently available for the diagnosis of infection by VT+ E. coli 0157.

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Section: Discussionmentioning

confidence: 53%

Detection of serum and faecal antibodies in haemorrhagic colitis caused by Escherichia coli O157

Siddons¹,

Chapman²

1993

Journal of Medical Microbiology

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“…Patients with yersiniosis or brucellosis have been shown to raise antibodies which react with the LPS of E. coli 0157 [this study, 13] [10]. Where serology alone provides evidence of infection these cross-reactions should be taken into consideration.…”

Section: Elisamentioning

confidence: 99%

“…This similarity in LPS composition has been used to explain antigenic cross-reactions between Y. enterocolitica 09 and B. abortus, and E. coli 0157 and B. abortus, detected with hyperimmune rabbit sera [10][11][12]. Sera from patients with yersiniosis have been shown to contain antibodies which react with B. abortus [4] and, similarly, patients with brucellosis raise antibodies which crossreact with E. coli 0157 [13]. Studies from this laboratory have shown that patients with HUS, caused by E. coli 0157, raise antibodies that react with the LPS of B. abortus [5,6].…”

Section: Introductionmentioning

confidence: 99%

The serological relationship betweenYersinia enterocoliticaO9 andEscherichia coliO157 using sera from patients with yersiniosis and haemolytic uraemic syndrome

Chart¹,

Cheasty²,

Cope

et al. 1991

Epidemiol. Infect.

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SUMMARYSera from patients with yersiniosis, shown to contain antibodies to Yersinia enterocolitica 09; and sera from patients with haemolytic uraemic syndrome (HUS) caused by Escherichia coli 0157, were used to investigate serological crossreactions between Y. enterocolitica 09 and E. coli 0157. Lipopolysaceharide (LPS) was isolated from strains of Y. enterocolitica 09 and E. coli 0157 and reacted with sera by immunoblotting and ELISA. Sera from patients with HUS contained antibodies to the LPS of E. coli 0157 only; 80 % of sera from patients with yersiniosis contained antibodies to the LPS of Y. enterocolitica 09 and E. coli 0157. This one-way cross-reaction was also detected using hyperimmune rabbit antisera.

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“…The presence of antibodies in sera from patients with HUS against the lipopolysaccharide (LPS) of E. coli O157 was reported initially by Notenboom et al (23). Subsequent studies designed to characterize the immune responses of patients infected with E. coli O157 have reported the presence of immunoglobulin M (IgM) (8), IgG (16), and both IgM and IgG (1) antibodies to O157 LPS in patients with HUS.…”

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confidence: 96%

Antibody Responses to Escherichia coli O157 and Other Lipopolysaccharides in Healthy Children and Adults

Navarro¹,

Eslava²,

Hernández³

et al. 2003

Clin Vaccine Immunol

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In Mexico, diarrheal disease due to different serotypes of Escherichia coli is highly prevalent, with only sporadic isolation of O157 non-H7 strains. This could be due to exposure to the O157 or related E. coli lipopolysaccharide (LPS), such as O7 or O116, at an early age. By using enzyme-linked immunosorbent assay (ELISA) and Western blotting, the present study analyzed 605 serum samples from Mexican adults and infants without clinical symptoms of disease for the presence of antibodies to these three E. coli LPSs. The bactericidal activities of homologous and heterologous rabbit and human serum samples against O7, O116, and O157 E. coli LPSs were also determined. By using a cutoff point of 0.7, it was found by the ELISAs that 28 of 562 (5%) of the serum samples from adolescents and adults and 2 of 43 (5%) of the serum samples from infants less than 1 year of age reacted with the O157 LPS. By using cutoff points between 0.4 and 0.699, the proportion of serum samples from both age groups that reacted with the O157 LPS increased to 20%. Western blotting analysis of selected serum samples that showed an intermediate response against the O157 LPS by the ELISAs showed that 61 of 88 (69%) reacted with the same LPS. A similar result was observed for maternal milk samples. The bactericidal activities of rabbit serum samples against the O7, O116, and O157 LPSs showed that they were positive for both homologous and heterologous antigens. Similar results were observed with the human serum samples. O157 non-H7 strains were identified in only 10% of the E. coli strains isolated from 263 Mexican children with and without diarrhea over the past 15 years. This absence of O157:H7 strains in Mexico may be associated with the presence of antibodies against O157 or related E. coli LPSs.Enterohemorrhagic Escherichia coli O157:H7 is of great clinical and epidemiological importance as a cause of hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS) in developed countries (2, 21, 35). The incidence of these bacteria in Mexico and other developing countries is low, although it has been reported that these bacteria are present in Chile, Brazil, Argentina, and Colombia (12,17,19,20).The presence of antibodies in sera from patients with HUS against the lipopolysaccharide (LPS) of E. coli O157 was reported initially by Notenboom et al. (23). Subsequent studies designed to characterize the immune responses of patients infected with E. coli O157 have reported the presence of immunoglobulin M (IgM) (8), IgG (16), and both IgM and IgG (1) antibodies to O157 LPS in patients with HUS. IgA antibodies have also been reported in the serum and feces of patients with HUS (6, 30). A study carried out by Reymond et al. (28) showed the presence of antibodies to O157 LPS in 12% of the population living in rural areas where O157 strains are endemic, whereas they were found in only 4.7% of the population living in urban areas. Similar results were observed by Belongia et al. (3) in a rural Wisconsin population.Cross-reactivities between the LPS of...

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Clinical Relevance of a Serological Cross-Reaction Between Escherichia Coli 0157 and Brucella Abortus

Cited by 21 publications

References 4 publications

Detection of serum and faecal antibodies in haemorrhagic colitis caused by Escherichia coli O157

Detection of serum and faecal antibodies in haemorrhagic colitis caused by Escherichia coli O157

The serological relationship betweenYersinia enterocoliticaO9 andEscherichia coliO157 using sera from patients with yersiniosis and haemolytic uraemic syndrome

Antibody Responses to Escherichia coli O157 and Other Lipopolysaccharides in Healthy Children and Adults

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