Clinical potential of microchip capillary electrophoresis systems

Colyer, Christa L.; Tang, Thompson; Chiem, Nghia; Harrison, David

doi:10.1002/elps.1150181006

Cited by 166 publications

(99 citation statements)

References 60 publications

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“…Analysis of physiological fluids and tissues using microfluidic devices presents a special challenge, both in terms of sensitivity and fouling of microchannels by matrix components. (Coyler et al, 1997) Microchips have been made with various configurations of channels, allowing for mixing, labeling, separation and detection all within the chip. Perhaps one of the most important factors in the successful resolution of any compound mixture is the design of the chip.…”

Section: Microchip Capillary Electrophoresismentioning

confidence: 99%

Application of Micro-Fluidic Devices for Biomarker Analysis in Human Biological Fluids

Kalish¹

2011

Biomedical Engineering, Trends, Research and Technologies

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Section: Microchip Capillary Electrophoresismentioning

confidence: 99%

Application of Micro-Fluidic Devices for Biomarker Analysis in Human Biological Fluids

Kalish¹

2011

Biomedical Engineering, Trends, Research and Technologies

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“…A variety of µ-TAS examples are included in Harrison and van den Berg (1998) and van den Berg et al (2000). For a review of microchip-based devices, see Cheng et al (1996a,b), Colyer et al (1997), Marshall and Hodgson (1997), Mastrangelo et al (1998), Figeys and Pinto (2000), and McDonald et al (2000).…”

Section: Mass Spectrometrymentioning

confidence: 99%

Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends

Meldrum¹

2000

Genome Res.

116

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Automation for genomics has enabled a 43-fold increase in the total finished human genomic sequence in the world in the past four years. This is the second half of a two-part, noncomprehensive review that presents an overview of different types of automation equipment used in genome sequencing. The first part of the review, published in the previous issue, focused on automated procedures used to prepare DNA for sequencing or analysis. This second part of the review presents a look at available DNA sequencers and array technology and concludes with a look at future technologies. Alternate sequencing technologies including mass spectrometry, biochips, and single molecule analysis are included in this review.Sequencing data, essential for many of the medical breakthroughs of today and tomorrow, is currently accumulating at an exponential rate, largely because of advances in sequencing methods and laboratory automation. Instruments are available that can automate nearly every step in the large-scale sequencing process.The first article in this two-part review (Meldrum 2000) presented a description of automation designed for isolating DNA, cloning or amplifying DNA, preparing enzymatic sequencing reactions, and purifying DNA. Part one of the review also showed that the majority of genome centers use both in-house automation and commercial automation (Collins et al. 1998;Wendl et al. 1998;Mullikin and McMurragy 1999;Spurr et al. 1999; see http://www.genome.org for a supplementary table providing a snapshot view of automation used at a number of different genome centers).In this, the second part of the review, the focus is on automation after DNA preparation and sequencing reactions-on obtaining the sequence and its analysis. A discussion of future technologies for DNA sequencing projects is also included. (See the web sites referenced for photos or further details on the instruments presented.)

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“…Over the last two decades, extensive progress has been made in both micro-and nano-technologies. In analytical chemistry, a micro-total analysis system (µ-TAS) 1,2 and a lab-on-a-chip concept, 3 which is based on a micro-fluidic channel chip, have been developed as new tools. Microchip devices with miniaturized electrophoresis for high-speed analysis were applied to the determination of DNA fragments, [4][5][6] amino acids, [7][8][9] proteins, [10][11][12][13] and so forth.…”

Section: Introductionmentioning

confidence: 99%

Fluorometric Determination of Sulfite and Nitrite in Aqueous Samples Using a Novel Detection Unit of a Microfluidic Device

et al. 2004

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On-chip fluorescence determination of sulfite and nitrite with N-(9-acridinyl)maleimide (NAM) and 2,3-diaminonaphthalene (DAN) has been developed using a novel fluorescence detection unit for microchip analysis. Usually, these fluorescence reagents are derivatized and detected separately in microchip analysis because different fluorescence wavelengths are emitted. The proposed fluorescence detection unit has optical fibers with no optical filter, and plural wavelengths of fluorescence were detected sensitively, even in the microchip. In this study, the simultaneous determination of sulfite and nitrite in environmental samples was performed with a polymer microchip analysis system. The calibration curves of sulfite and nitrite showed linear relations (R 2 = 0.998 (sulfite) and R 2 = 0.990 (nitrite)), and the relative standard deviations (RSD) for 4 runs were 2.1% (20 µM sulfite) and 1.3% (20 µM nitrite), respectively. The proposed method was applied to the recovery test of sulfite and nitrite in environmental samples.

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Clinical potential of microchip capillary electrophoresis systems

Cited by 166 publications

References 60 publications

Application of Micro-Fluidic Devices for Biomarker Analysis in Human Biological Fluids

Application of Micro-Fluidic Devices for Biomarker Analysis in Human Biological Fluids

Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends

Fluorometric Determination of Sulfite and Nitrite in Aqueous Samples Using a Novel Detection Unit of a Microfluidic Device

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