Clinical performance of a specific granulocyte colony stimulating factor ELISA to determine its concentration in follicular fluid as a predictor of implantation success during<i>in vitro</i>fertilization

Tournaye, Herman; D’Hooghe, Thomas; Verheyen, Greta; Devreker, K. F.; d'HAUTERIVE, Sophie Perrier; Nisolle, Michelle; Foidart, Jean‐Michel; Munaut, Carine; Noël, Laure

doi:10.1080/09513590.2019.1631283

Cited by 8 publications

(3 citation statements)

References 15 publications

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“…The latter statement is supported by studies that reveal a positive correlation of G‐CSF levels in FF with implantation outcome 25,26 . This is in accordance with recent findings that report an association of increased FF G‐CSF levels with high probabilities of implantation and live birth rates 27,28 …”

Section: Introductionsupporting

confidence: 94%

“…On top of that, a study conducted by Chimote and colleagues indicated that FF G‐CSF levels appear to be a biomarker of paramount importance in predicting blastocyst formation, with almost 91% sensitivity and 73% specificity 78 . Furthermore, various studies have demonstrated a positive association of FF G‐CSF levels with fertilization and pregnancy rates, 79 along with implantation rates 26–28 . Analyzing pregnancy outcome, our data show more pregnancies for normal responders, which had nine successful cases of CPs, in contrast with the poor responder group, which reported five.…”

Section: Discussionmentioning

confidence: 52%

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Investigating apoptotic, inflammatory, and growth markers in poor responders undergoing natural in vitro fertilization cycles: a pilot study

Sfakianoudis¹,

Tsioulou

Maziotis

et al. 2020

Annals of the New York Academy of Sciences

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This study investigates follicular fluid (FF) from patients with poor and normal ovarian response undergoing natural assisted reproductive technology cycles. We report about (1) cell‐free DNA (cfDNA), which reflects apoptosis; (2) corticotropin‐releasing hormone (CRH); (3) interleukin (IL)‐15, which reflects inflammation; (4) granulocyte colony–stimulating factor (G‐CSF); (5) vascular endothelial growth factor (VEGF); and (6) insulin‐like growth factor I (IGF‐I), which reflects follicular growth. Forty‐four poor responders and 44 normal responders—according to the Bologna criteria—were recruited. FF samples were prepared for cfDNA quantification employing Q‐PCR and for CRH, IL‐15, G‐CSF, VEGF, and IGF‐I quantification employing ELISA. Statistically nonsignificant different levels of FF cfDNA, CRH, IL‐15, VEGF, and IGF‐I were observed. Interestingly, statistically significant higher G‐CSF levels were observed in normal responders (302.48 ± 474.36 versus 200.10 ± 426.79 pg/mL, P = 0.003). Lower cfDNA integrity was observed in cycles resulting in clinical pregnancy for both groups (normal: 0.07 ± 0.04 versus 0.25 ± 0.17 ng/μL, P < 0.001; poor: 0.10 ± 0.06 versus 0.26 ± 0.12 ng/μL, P < 0.001). The results predominantly showcase similarities between normal and poor responders pertaining to inflammatory, apoptotic, and growth factors. This may be attributed to the employment of natural cycles in order to exclude controlled ovarian stimulation as a factor—indicating its detrimental effect. As G‐CSF levels presented significantly higher in normal responders, its vital role in understanding a compromised ovarian response is highlighted.

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Section: Introductionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 52%

Investigating apoptotic, inflammatory, and growth markers in poor responders undergoing natural in vitro fertilization cycles: a pilot study

Sfakianoudis¹,

Tsioulou

Maziotis

et al. 2020

Annals of the New York Academy of Sciences

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show abstract

“…G-CSF also has a recognized role in several aspects of human reproduction [13][14][15][16]. In a recent trial with an ultra-sensitive ELISA specifically designed for the quantification of follicular G-CSF, the probability of successful implantation was 3.3-fold higher for embryos derived from follicles with high G-CSF levels than for those derived from follicles with low G-CSF levels [17].…”

Section: Introductionmentioning

confidence: 99%

A paracrine interaction between granulosa cells and leukocytes in the preovulatory follicle causes the increase in follicular G-CSF levels

Noël

Fransolet

Jacobs

et al. 2020

J Assist Reprod Genet

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Objective Follicular granulocyte colony-stimulating factor (G-CSF) is a new biomarker of oocyte quality and embryo implantation in in vitro fertilization (IVF) cycles. Its role in reproduction is poorly understood. Our study aimed to investigate the mechanisms and cells responsible for G-CSF production in the preovulatory follicle. Design Laboratory research study. Setting Single-center study. Interventions Granulosa cells and leukocytes were isolated from the follicular fluids (FF) or the blood of women undergoing IVF and from the blood of a control group of women with spontaneous ovulatory cycles to perform cocultures. Main outcome measure G-CSF-secreted protein was quantified in the conditioned media of cocultures. Results G-CSF secretion was considerably increased in cocultures of granulosa cells and leukocytes. This effect was maximal when leukocytes were isolated from the blood of women in the late follicular phase of the menstrual cycle or from the FF of women undergoing IVF. The leukocyte population isolated from the FF samples of women undergoing IVF had a higher proportion of granulocytes than that isolated from the corresponding blood samples. Leukocytes induced the synthesis and secretion of G-CSF by granulosa cells. Among a range of other FF cytokines/chemokines, only growth-regulated oncogene alpha (GROα) was also increased. Conclusion The notable rise in G-CSF at the time of ovulation coincides with the accumulation of follicular granulocytes, which stimulate G-CSF production by granulosa cells via paracrine interactions. High follicular G-CSF concentrations may occur in follicles with optimal granulosa-leukocyte interactions, which could explain the increased implantation rate of embryos arising from these follicles.

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Granulocyte colony-stimulating factor in reproductive-related disease: Function, regulation and therapeutic effect

Ding

Wang

Cai

et al. 2022

Biomedicine & Pharmacotherapy

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Clinical performance of a specific granulocyte colony stimulating factor ELISA to determine its concentration in follicular fluid as a predictor of implantation success duringin vitrofertilization

Cited by 8 publications

References 15 publications

Investigating apoptotic, inflammatory, and growth markers in poor responders undergoing natural in vitro fertilization cycles: a pilot study

Investigating apoptotic, inflammatory, and growth markers in poor responders undergoing natural in vitro fertilization cycles: a pilot study

A paracrine interaction between granulosa cells and leukocytes in the preovulatory follicle causes the increase in follicular G-CSF levels

Granulocyte colony-stimulating factor in reproductive-related disease: Function, regulation and therapeutic effect

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